O2k-Virtual Support self-study material: Difference between revisions
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:::* [[Sample]] Β | :::* [[Sample]] Β | ||
:::* [[Mitochondrial preparations]] Β | :::* [[Mitochondrial preparations]] Β | ||
:::* [[MiPNet17.03 Shredder vs Fibres]] Β | :::* [[MiPNet17.03 Shredder vs Fibres|Shredder ''vs'' Fibers]] Β | ||
:::* [https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be O2k-Videosupport: Shredder tissue homogenate] Β | :::* [https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be O2k-Videosupport: Shredder tissue homogenate] Β | ||
:::* [https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be O2k-Videosupport: Fiber permeabilization] Β | :::* [https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be O2k-Videosupport: Fiber permeabilization] Β | ||
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=== Part 2.2: Biological experiment and data analysis === Β | === Part 2.2: Biological experiment and data analysis === Β | ||
:::* [[MiPNet19. | :::* [[MiPNet19.14 SOP Hamilton_microsyringes|SOP: Hamilton microsyringes]] | ||
:::* [[MiPNet09. | :::* [[MiPNet09.12 O2k-Titrations|O2k-Titrations]] | ||
:::* [https://www.youtube.com/watch?v=9j4ZtHmHissΒ O2k-Videosupport:Β How to perform an experiment with a SUIT DL-Protocol (DLP)] Β | :::* [https://www.youtube.com/watch?v=9j4ZtHmHissΒ O2k-Videosupport:Β How to perform an experiment with a SUIT DL-Protocol (DLP)] Β | ||
:::* [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4| | :::* [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4|Oxygen flux analysis]] Β | ||
:::* [https://www.youtube.com/watch?v=HRAGvmaK01I O2k-Videosupport: O<sub>2</sub> flux analysis with DatLab 7.4] Β | :::* [https://www.youtube.com/watch?v=HRAGvmaK01I O2k-Videosupport: O<sub>2</sub> flux analysis with DatLab 7.4] Β | ||
Revision as of 15:24, 3 November 2021
O2k-Virtual Support self-study material |
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
General information
- To benefit optimally from the tutored training sessions of the Virtual O2k-Workshop, please make yourself familiar with Materials for self-study in advance.
O2k-Basic
Part 1.1: OroboPOS service and O2k instrumental
- O2k-Manual: O2k-FluoRespirometer
- O2k-Manual: POS Service
- Step 1: O2k-Videosupport: OroboPOS
- Step 2: O2k-Videosupport: Cathode cleaning
- Step 3: O2k-Videosupport: Anode cleaning
- Step 4: O2k-Videosupport: Membrane mounting
- Step 5: O2k-Videosupport: Start DatLab 7
- Step 6: O2k-Videosupport: Insert OroboPOS
- Step 7: O2k-Videosupport: Insert O2k Chamber
- Step 8: O2k-Videosupport: O2k Chamber volume calibration
Part 1.2: DatLab
Part 1.3: O2 calibration and instrumental background
Part 2.1: Biological samples and experimental design
Pathway control and coupling control of mitochondrial respiration:
Sample types,mitochondrial preparations and sample addition:
SUIT protocols, SUITbrowser, DL-Protocols:
Part 2.2: Biological experiment and data analysis
O2k-Advanced
O2k-Applications: Simultaneous determination of O2 and H2O2 fluxes
NextGen-O2k KOL Training
(R) These materials will be sent upon registration
- PB-Module Manual (R)
- O2k-Videosupport: SUIT-030_PB_ce_D069 demo experiment (R)
- DL-Protocol: SUIT-030_PB_ce_D069 (R)
- O2k-Videosupport: SUIT-030_PB_ce_D070 demo experiment (R)
- DL-Protocol: SUIT-030_PB_ce_D070 (R)
- Q-Module
- Q-Module manual: MiPNet24.12 NextGen-O2k: Q-Module
- CV software manual: MiPNet24.16 DatLab8.0: CV-Module
- Videos: Q-Module