MiPNet19.15 IOC97: Difference between revisions

Latest revision as of 15:13, 8 June 2020

MiPNet19.15 IOC97

Lausanne CH, 2014 Sep. 25-26 Oroboros O2k-Workshop on HRR and O2k-Fluorometry, IOC97.

»

Oroboros (2014-09-25) Mitochondr Physiol Network

Abstract:

97th Oroboros O2k-Workshop on high-resolution respirometry and O2k-Fluorometry

• O2k-Network Lab: CH Lausanne Sandi C, AT Innsbruck Oroboros

Labels:

HRR: Oxygraph-2k

ORO, IOC, 2014, O2k-Network

» MitoPedia: Respiratory states

Lecturer

Gnaiger Erich, CEO, Oroboros Instruments

Programme

Programme MiPNet19.15.pdf

Thursday, September 25

09:00 – 10:30 A new Power-O2k Lab of Prof. Carmen Sandi: 4 O2k.

Instrumental setup, chamber assembly, OroboPOS – sensor service, quality control (group Carmen Sandi).

10:30 Break – Registration of external participants

11:00 – 11:15 Erich Gnaiger:

Welcome to external participants. Oroboros Instruments and perspectives of mitochondrial physiology.

11:15 – 11:30 Introduction of participants: who is who?

11:30 – 12:30 Basic and new features of DatLab 6.

Oxygen calibration.
Oxygen flux and instrumental tests.

12:30 Lunch

13:30 – 15:30 Experimental design.

Cell ergometry: from intact cells to mt-preparations.
A challenge for simultaneous measurements of respiration and mt-membrane potential: solving a puzzle.

15:30 Break

16:00 – 17:30 Titration-Injection microPump TIP2k:

From automatic instrumental background tests to steady-state control in respiratory experiments.

17:30 – 18:00 Q&A session on HRR and OXPHOS analysis

Design of experimental protocol - day 2.

Friday, September 26

09:00 – 10:30 Experiment

HRR and O2k-Fluorometry – respiration and H2O2 production.

10:30 Break

11:00 – 12:30 Experiment continued.

12:30 Lunch

13:15 – 15:30 DatLab analysis.

15:30 Break

16:00 – 16:30 Trouble shooting.

16:30 – 17:30 Normalization of mt-respiration: flux control states and flux control steps.

17:30 - 18:00 Feedback – conclusions – stay connected as a MiPNet Lab.

Venue

Laboratory of Behavioral Genetics

Brain Mind Institute

Ecole Polytechnique Federale de Lausanne

Auditorium/Meeting room

CH-1015 Lausanne

Contact: Prof. Carmen Sandi, Director. Email: [email protected]

Registration and general information

[email protected]

Oroboros Instruments

high-resolution respirometry

Schöpfstrasse 18

A-6020 Innsbruck, Austria

Tel: +43 512 566796

Fax: +43 512 566796 20

Participants

Name	Institution
Canto_AC (Thursday, Friday)	CH Lausanne Canto C: Nestlé Institute of Health Sciences SA, EPFL
Eggimann Sandra	CH Bern Nuoffer JM: Universitätsinstitut für klinische Chemie, Inselspital Bern
Grosse Jocelyn (Thursday)	CH Lausanne Sandi C: Brain Mind Institute, EPFL
Hahn Dagmar	CH Bern Nuoffer JM: Universitätsinstitut für klinische Chemie, Inselspital Bern
Hollis Fiona (Thursday afternoon and Friday)	CH Lausanne Sandi C: Brain Mind Institute, EPFL
Huzard Damien (Thursday morning and Friday morning)	CH Lausanne Sandi C: Brain Mind Institute, EPFL
Meng Kévin (Thursday afternoon and Friday morning)	CH Lausanne Sandi C: Brain Mind Institute, EPFL
Kulkarni Sameer	CH Lausanne Canto C: Nestlé Institute of Health Sciences SA, EPFL
Papilloud Aurélie (Thursday and Friday)	CH Lausanne Sandi C: Brain Mind Institute, EPFL
Ratajczak Joanna (Thursday afternoon and Friday)	CH Lausanne Canto C: Nestlé Institute of Health Sciences SA, EPFL
Riccio Orbicia (Thursday afternoon and Friday)	CH Lausanne Sandi C: Brain Mind Institute, EPFL
Sandi Carmen	CH Lausanne Sandi C: Brain Mind Institute, EPFL
Walker Sophie (Thursday)	CH Lausanne Sandi C: Brain Mind Institute, EPFL
Zanoletti Olivia (Thursday and Friday)	CH Lausanne Sandi C: Brain Mind Institute, EPFL

Recommended reading

Key references

Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. »Bioblast Access

Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58. »Bioblast Access

Introduction and O2k-Core Manual

»O2k-Core Manual.pdf

HRR and O2k-Fluorometry

Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. »Pubmed 24862266

Mitochondrial pathways

Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck: 80 pp. »Open Access

O2k-Workshops are listed as MitoGlobal Events.

@@ Line 1: / Line 1: @@
-{{Mainpage OROBOROS}}
+{{OROBOROS header page name}}
 {{Publication
-|title=[[Image:O2k-WorldWide.jpg|right|60px|link=http://www.bioblast.at/index.php/MiPNet_Reference_Laboratories|MiPNet Reference Laboratories]]
+|title=[[Image:O2k-Network.png|right|60px|link=O2k-Network|O2k-Network]]
-'''Lausanne CH''', 2014 Sep. 24 OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, '''IOC97.'''
+'''Lausanne CH''', 2014 Sep. 25-26 Oroboros O2k-Workshop on HRR and O2k-Fluorometry, '''IOC97.'''
-|info=[[Media:MiPNet19.15 |'''MiPNet19.15.pdf''']]
+|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/1/1d/MiPNet19.15_IOC97_2014-09_Lausanne.pdf |Bioblast pdf]]
-|authors=OROBOROS
+|authors=Oroboros
-|year=2014-09-24
+|year=2014-09-25
 |journal=Mitochondr Physiol Network
-|abstract=[[Image:O2k-Workshops.png|left|130px|link=http://www.oroboros.at/?O2k-Workshops]]
+|abstract=[[Image:O2k-Workshops.png|left|130px|link=O2k-Workshops|O2k-Workshops]]
-'''97th OROBOROS O2k-Workshop on high-resolution respirometry and O2k-Fluorometry'''
+'''97th Oroboros O2k-Workshop on high-resolution respirometry and O2k-Fluorometry'''
-|mipnetlab=CH Lausanne Sandi C, AT Innsbruck OROBOROS
+|mipnetlab=CH Lausanne Sandi C, AT Innsbruck Oroboros
 }}
-Please note: '''The exact dates of this workshop are not set'''
 {{Labeling
-|area=Respiration
+|instruments=Oxygraph-2k
-|topics=
+|additional=ORO, IOC, 2014, O2k-Network
-|couplingstates=
-|instruments=Oxygraph-2k, Fluorometry
-|additional=ORO, IOC, 2014, MiPNet, Next
 }}
-'''»''' [[Bioblast Glossary: Respiratory states]]
+'''»''' [[MitoPedia: Respiratory states]]
-[[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:E.jpg|link=ETS capacity|ETS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:L.jpg|link=LEAK respiration|LEAK]] [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]
+[[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link=ET capacity|ETS]] [[Image:L.jpg|link=LEAK respiration|LEAK]] [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]
 __TOC__
 == Lecturer ==
-[[File:Gnaiger Erich.jpg|left|80px|Erich Gnaiger]]
+[[File:Gnaiger Erich.jpg|80px|Erich Gnaiger]]
-[[Gnaiger Erich]], CEO, OROBOROS INSTRUMENTS
+[[Gnaiger Erich]], CEO, Oroboros Instruments
+== Programme ==
+ [[Media:MiPNet19.15_IOC97_2014-09_Lausanne.pdf |'''Programme MiPNet19.15.pdf''']]
+=== Thursday, September 25 ===
+:00 – 10:30 A new Power-O2k Lab of Prof. Carmen Sandi: 4 O2k.
+::::* Instrumental setup, chamber assembly, OroboPOS – sensor service, quality control (group Carmen Sandi).
+:30 ''Break – Registration of external participants''
+:00 – 11:15	Erich Gnaiger:
+:::: Welcome to external participants. Oroboros Instruments and perspectives of mitochondrial physiology.
+:15 – 11:30	Introduction of participants: who is who?
+:30 – 12:30 Basic and new features of DatLab 6.
+::::* Oxygen calibration.
+::::* Oxygen flux and instrumental tests.
+:30	Lunch
+:30 – 15:30 Experimental design.
+::::* Cell ergometry: from intact cells to mt-preparations.
+::::* A challenge for simultaneous measurements of respiration and mt-membrane potential: solving a puzzle.
+:30 	Break
+:00 – 17:30 Titration-Injection microPump TIP2k:
+:::: From automatic instrumental background tests to steady-state control in respiratory experiments.
+:30 – 18:00 Q&A session on HRR and OXPHOS analysis
+:::: Design of experimental protocol - day 2.
+=== Friday, September 26 ===
+:00 – 10:30 Experiment
+:::: HRR and O2k-Fluorometry – respiration and H2O2 production.
+:30	Break
+:00 – 12:30 Experiment continued.
+:30	Lunch
+:15 – 15:30 DatLab analysis.
+:30 	Break
+:00 – 16:30 Trouble shooting.
-== Programme ==
+:30 – 17:30 Normalization of mt-respiration: flux control states and flux control steps.
+:30 - 18:00 Feedback – conclusions – stay connected as a MiPNet Lab.
 === Venue ===
+Laboratory of Behavioral Genetics
+Brain Mind Institute
+Ecole Polytechnique Federale de Lausanne
+Auditorium/Meeting room
+CH-1015 Lausanne
+Contact: Prof. Carmen Sandi, Director. Email: [email protected]
@@ Line 48: / Line 98: @@
-OROBOROS INSTRUMENTS
+Oroboros Instruments
 high-resolution respirometry
@@ Line 59: / Line 109: @@
 Fax: +43 512 566796 20
+== Participants ==
+{| class="wikitable"
+|-
+! Name
+! Institution
+|-
+|[[Canto_AC]] (Thursday, Friday)
+|'''[[CH Lausanne Canto C]]:''' Nestlé Institute of Health Sciences SA, EPFL
+|-
+|[[Eggimann Sandra]]
+|'''[[CH Bern Nuoffer JM]]:''' Universitätsinstitut für klinische Chemie, Inselspital Bern
+|-
+|'''Grosse Jocelyn''' (Thursday)
+|'''[[CH Lausanne Sandi C]]:''' Brain Mind Institute, EPFL
+|-
+|[[Hahn Dagmar]]
+|'''[[CH Bern Nuoffer JM]]:''' Universitätsinstitut für klinische Chemie, Inselspital Bern
+|-
+|'''[[Hollis Fiona]]''' (Thursday afternoon and Friday)
+|'''[[CH Lausanne Sandi C]]:''' Brain Mind Institute, EPFL
+|-
+|'''Huzard Damien''' (Thursday morning and Friday morning)
+|'''[[CH Lausanne Sandi C]]:''' Brain Mind Institute, EPFL
+|-
+|'''Meng Kévin''' (Thursday afternoon and Friday morning)
+|'''[[CH Lausanne Sandi C]]:''' Brain Mind Institute, EPFL
+|-
+|'''Kulkarni Sameer'''
+|'''[[CH Lausanne Canto C]]:''' Nestlé Institute of Health Sciences SA, EPFL
+|-
+|'''Papilloud Aurélie''' (Thursday and Friday)
+|'''[[CH Lausanne Sandi C]]:''' Brain Mind Institute, EPFL
+|-
+|'''Ratajczak Joanna'''  (Thursday afternoon and Friday)
+|'''[[CH Lausanne Canto C]]:''' Nestlé Institute of Health Sciences SA, EPFL
+|-
+|'''Riccio Orbicia''' (Thursday afternoon and Friday)
+|'''[[CH Lausanne Sandi C]]:''' Brain Mind Institute, EPFL
+|-
+|'''[[Sandi Carmen]]'''
+|'''[[CH Lausanne Sandi C]]:''' Brain Mind Institute, EPFL
+|-
+|'''Walker Sophie''' (Thursday)
+|'''[[CH Lausanne Sandi C]]:''' Brain Mind Institute, EPFL
+|-
+|'''Zanoletti Olivia''' (Thursday and Friday)
+|'''[[CH Lausanne Sandi C]]:''' Brain Mind Institute, EPFL
+|}
@@ Line 64: / Line 165: @@
 <big>'''Key references'''</big>
-[[Image:O2k-Publications.jpg|left|50px|link=http://www.bioblast.at/index.php/O2k-Publications: Topics|O2k-Publications in the MiPMap]]
+[[Image:O2k-Publications.jpg|left|50px|link=O2k-Publications: Topics|O2k-Publications in the MiPMap]]
-:* Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542: 163-181. [[Krumschnabel 2014 Methods Enzymol |»Pubmed 24862266]]
+: Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. [[Gnaiger_2008_POS |»Bioblast Access]]
-:* Perry CG, Kane DA, Lanza IR, Neufer PD (2013) Methods for assessing mitochondrial function in diabetes. Diabetes 62: 1041-1053. [[Perry 2013 Diabetes |»PMID: 23520284]]
+:* Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58. [[Pesta 2012 Methods Mol Biol |»Bioblast Access]]
-:* Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58. [[Pesta 2012 Methods Mol Biol |»Bioblast Access]]
 <big>'''Introduction and O2k-Core Manual'''</big>
-[[Image:O2k-Manual.jpg|left|30px|link=http://oroboros.at/?O2k-Manual|O2k-Manual]]
+[[Image:O2k-Manual.jpg|left|30px|link=O2k-Manual|O2k-Manual]]
-* Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352. [[Gnaiger_2008_POS |»Bioblast Access]]
+: [[Media:MiPNet19.01 O2k-Core Manual.pdf|'''»O2k-Core Manual.pdf''']]
-* [[Media:MiPNet19.01 O2k-Core Manual.pdf|'''»O2k-Core Manual.pdf''']]
 <big>'''HRR and O2k-Fluorometry'''</big>
-* »[[MiPNet17.05 O2k-Fluorescence LED2-Module |Manual: O2k-Fluorescence LED2-Module]]
+[[Image:O2k-Fluorometer.JPG|60px|left|link=O2k-Fluorometer|O2k-Fluorometer]]
-* Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01): 1-6. [[MiPNet18.05 Amplex-Mouse-heart |»Open Access]]
+: Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. [[Krumschnabel 2014 Methods Enzymol |»Pubmed 24862266]]
-* »[[O2k-Publications: O2k-Fluorometry|O2k-Fluorometry]]'''
+:* »[[MiPNet17.05 O2k-Fluo LED2-Module |Manual: O2k-Fluo LED2-Module]]
+:* »[[O2k-Publications: O2k-Fluorometry|O2k-Fluorometry]]'''
 <big>'''Mitochondrial pathways'''</big>
 [[Image:O2k-Protocols.jpg|left|40px|link=http://oroboros.at/?O2k-Protocols|O2k-Protocols]]
-Gnaiger E (2012) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 3rd ed. Mitochondr Physiol Network 17.18. OROBOROS MiPNet Publications, Innsbruck: 64 pp. [[Gnaiger 2012 MitoPathways |»Open Access]] - '''''handout to O2k-Workshop participants'''''.
+: Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck: 80 pp. [[Gnaiger 2014 MitoPathways |»Open Access]]
-[[Image:MitoGlobal.jpg|right|80px|link=http://www.bioblast.at/index.php/MitoGlobal|MitoGlobal]]
+[[Image:MitoGlobal.jpg|right|80px|link=MitoGlobal|MitoGlobal]]
   O2k-Workshops are listed as [[MitoGlobal Events]].
 [[Category:O2k-Workshops]]