Template Select fluorescence sensor: Difference between revisions
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:::: Switching between different excitation wavelengths and filters is achieved by simply exchanging the Fluo-Sensors. Two types of optical sensors are supplied with different LEDs for fluorescence excitation, and the effective spectra of the LEDs are modified by filters. | :::: Switching between different excitation wavelengths and filters is achieved by simply exchanging the Fluo-Sensors. Two types of optical sensors are supplied with different LEDs for fluorescence excitation, and the effective spectra of the LEDs are modified by filters. | ||
:::: Select the Fluo-Sensor and filter set from the table on [https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module#Fluorophores application-specific settings] (here we should link to the respective section which is also a template). Each Fluo-Sensor is delivered with a mounted filter set: | :::: Select the Fluo-Sensor and filter set from the table on [https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module#Fluorophores application-specific settings] (here we should link to the respective section which is also a template). Each Fluo-Sensor is delivered with a mounted filter set: | ||
::::# Fluo-Sensor Green (links to product pages)[[Smart Fluo-Sensor Green]]: 525 nm (dominant wavelength). The installed Filter-Cap is ready for H<sub>2</sub>O<sub>2</sub> measurement with [[Amplex red|Amplex® UltraRed]] or for measurements of mt-membrane potential with [[TMRM]]. | ::::# Fluo-Sensor Green (links to product pages?)[[Smart Fluo-Sensor Green]]: 525 nm (dominant wavelength). The installed Filter-Cap is ready for H<sub>2</sub>O<sub>2</sub> measurement with [[Amplex red|Amplex® UltraRed]] or for measurements of mt-membrane potential with [[TMRM]]. | ||
::::# Fluo-Sensor Blue (links to product pages? [[Smart Fluo-Sensor Blue]]: 465 nm (dominant wavelength). The installed Filter-Cap is ready for measurements of mt-membrane potential with [[safranin]] or [[Rhodamine 123]]. A different filter is used for measurement with [[Magnesium green|Magnesium Green™]] or Calcium Green™. | ::::# Fluo-Sensor Blue (links to product pages?) [[Smart Fluo-Sensor Blue]]: 465 nm (dominant wavelength). The installed Filter-Cap is ready for measurements of mt-membrane potential with [[safranin]] or [[Rhodamine 123]]. A different filter is used for measurement with [[Magnesium green|Magnesium Green™]] or Calcium Green™. | ||
Revision as of 17:13, 18 November 2021
Select the Fluo-Sensors
- Switching between different excitation wavelengths and filters is achieved by simply exchanging the Fluo-Sensors. Two types of optical sensors are supplied with different LEDs for fluorescence excitation, and the effective spectra of the LEDs are modified by filters.
- Select the Fluo-Sensor and filter set from the table on application-specific settings (here we should link to the respective section which is also a template). Each Fluo-Sensor is delivered with a mounted filter set:
- Fluo-Sensor Green (links to product pages?)Smart Fluo-Sensor Green: 525 nm (dominant wavelength). The installed Filter-Cap is ready for H2O2 measurement with Amplex® UltraRed or for measurements of mt-membrane potential with TMRM.
- Fluo-Sensor Blue (links to product pages?) Smart Fluo-Sensor Blue: 465 nm (dominant wavelength). The installed Filter-Cap is ready for measurements of mt-membrane potential with safranin or Rhodamine 123. A different filter is used for measurement with Magnesium Green™ or Calcium Green™.
