Template:SUIT-001
Step | Respiratory state | Pathway control | ET-Complex entry into Q-junction | Comment |
---|---|---|---|---|
1PM | PML | N | CI | PML or PM_L: Pyruvate & Malate, N-LEAK respiration, NL
NADH-linked substrates (type N-pathway to Q). Template:SUIT L n |
2D | PMP | N | CI | PMP or PM_P: N-OXPHOS capacity, NP
OXPHOS capacity, P (with saturating [ADP]), with NADH-linked substrates. |
2c | PMcP | N | CI | PMcP or PMc_P: Cytochrome c test for quality control
Addition of cytochrome c yields a test for integrity of the mtOM. Stimulation by added cytochrome c would indicate an injury of the mtOM and limitation of respiration in state NP due to loss of cytochrome c (P, OXPHOS capacity with type N substrates). Cytochrome c is added immediately after the earliest ADP-activation step. |
3U | PME | N | CI | PME or PM_E: N-ET capacity, NE
Uncoupler titration (avoiding inhibition by high uncoupler concentrations) to obtain electron transfer (ET) capacity (noncoupled ET-state), as a test for limitation of OXPHOS-capacity by the phosphorylation system (ANT, ATP synthase, phosphate transporter) relative to ET-capacity, E. |
4G | PGM(E) | N | CI | |
5S | PGMSE | NS | CI&II | GMSP or GMS_P: NS-OXPHOS capacity, NSP
Respiratory stimulation by further addition of succinate, S, to type N substrates, with convergent electron flow in the NS-pathway (CI&II-linked pathway to the Q-junction) for reconstitution of TCA cycle function, in the coupled state as an estimate of OXPHOS-capacity, P. |
6Oct | OctPGMS(E) | FNS | FAO&CI&II | |
7Rot | SE | S | CII | SE or S_E: S-ET capacity
S-pathway ET-capacity after blocking CI with rotenone, inhibiting the N- and F-pathways. |
8Gp | SGp(E) | SGp | CII&GpDH | |
9Ama | ROX | ROX: residual oxygen consumption
Rox is due to oxidative side reactions, estimated after addition of Antimycin A (inhibitor of CIII). Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt). |
Step | Respiratory state | Pathway control | ET-Complex | Comment |
---|---|---|---|---|
## AsTm | AsTmE | CIV | CIV | |
## Azd | CHB |