Template:Keywords: Coupling control

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  The Blue Book 2020
  

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  OXPHOS-, ROUTINE-, ET-, and LEAK states; respiratory capacities (P, R, E, L) corrected for residual oxygen consumption Rox.

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  4-compartmental OXPHOS model. (1) ET capacity E of the noncoupled electron transfer system ETS. OXPHOS capacity P is partitioned into (2) the dissipative LEAK component L, and (3) ADP-stimulated P-L net OXPHOS capacity. (4) If P-L is kinetically limited by a low capacity of the phosphorylation system to utilize the protonmotive force pmF, then the apparent E-P excess capacity is available to drive coupled processes other than phosphorylation P» (ADP to ATP) without competing with P».

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  (P-L)/P is the OXPHOS P-L control efficiency. The biochemical coupling efficiency is independent of kinetic control by the phosphorylation system when expressed as the E-L coupling efficiency, (E-L)/E. (E-P)/E is the kinetic E-P control efficiency.

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Mitochondrial and cellular respiratory rates in coupling-control states

» Baseline state

Respiratory rate	Definition	Icon
OXPHOS-capacity	P = P´-Rox
ROUTINE-respiration	R = R´-Rox
ET-capacity	E = E´-Rox		» Level flow
			» Noncoupled respiration - Uncoupler
LEAK-respiration	L = L´-Rox		» Static head
			» LEAK-state with ATP
			» LEAK-state with oligomycin
			» LEAK-state without adenylates
Residual oxygen consumption Rox	L = L´-Rox

• Chance and Williams nomenclature: respiratory states

» State 1 —» State 2 —» State 3 —» State 4 —» State 5

Flux-control ratios related to coupling in mt-preparations and living cells

» Flux-control ratio

» Coupling-control ratio

» Coupling-control protocol

FCR	Definition	Icon
L/P coupling-control ratio	L/P		» Respiratory acceptor control ratio, RCR = P/L
L/R coupling-control ratio	L/R
L/E coupling-control ratio	L/E		» Uncoupling-control ratio, UCR = E/L
P/E control ratio	P/E
R/E control ratio	R/E
net P/E control ratio	(P-L)/E
net R/E control ratio	(R-L)/E

Net, excess, and reserve capacities of respiration

Respiratory net rate	Definition	Icon
Net OXPHOS-capacity P-L	P-L
Net ROUTINE-activity R-L
Net ET-capacity E-L	E-L
ET-excess capacity E-P	E-P
ET-reserve capacity E-R	E-R

Flux-control efficiencies related to coupling-control ratios

» Flux-control efficiency j_Z-Y

» Background state

» Reference state

» Metabolic control variable

Coupling-control efficiency		Definition		Icon
OXPHOS-coupling efficiency P-L	jP-L	= (P-L)/P	= 1-L/P		» Biochemical coupling efficiency
ROUTINE-coupling efficiency R-L	jR-L	= (R-L)/R	= 1-L/R
ET-coupling efficiency E-L	jE-L	= (E-L)/E	= 1-L/E		» Biochemical coupling efficiency
ET-excess control efficiency E-P	jE-P	= (E-P)/E	= 1-P/E
ET-reserve control efficiency E-R	jE-R	= (E-R)/E	= 1-R/E

General

» Basal respiration

» Dyscoupled respiration

» Dyscoupling

» Electron leak

» Electron-transfer-pathway state

» Oxidative phosphorylation

» Oxygen flow

» Oxygen flux

» Permeabilized cells

» Phosphorylation system

» Proton leak

» Proton slip

» Respiratory state

» Uncoupling