Substrate-uncoupler-inhibitor titration: Difference between revisions
No edit summary |
No edit summary |
||
Line 21: | Line 21: | ||
* [[Gnaiger_2007_MitoPathways]] | * [[Gnaiger_2007_MitoPathways]] | ||
* [[Boushel_2007_Diabetologia]] | * [[Boushel_2007_Diabetologia]] | ||
* [[ | * [[Gnaiger 2009 Int J Biochem Cell Biol]] | ||
* [[Sjoevall_2010_CritCare]] | * [[Sjoevall_2010_CritCare]] | ||
* [[Pesta_2012_Methods Mol | * [[Pesta_2012_Methods Mol Biol]] | ||
* [[Pesta_2011_AJP]] | * [[Pesta_2011_AJP]] | ||
* [[Lemieux_2011_Int | * [[Lemieux_2011_Int J Biochem Cell Biol]] |
Revision as of 19:11, 17 February 2012
- high-resolution terminology - matching measurements at high-resolution
Substrate-uncoupler-inhibitor titration
Description
Mitochondrial SUIT protocols are used with mt-preparations to study respiratory control in a sequence of coupling and substrates states induced by multiple titrations.
Abbreviation: SUIT
Reference: MiPNet12.12, MiPNet09.12, Gnaiger_2007_MitoPathways
MitoPedia methods:
Respirometry
MitoPedia topics: "Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
Respiratory state"Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
SUIT protocols
SUIT protocols have been developed for the first time on the basis of high-resolution respirometry, since their application depends critically on long-term stability of the polarographic oxygen sensor (experiments may be extended over 1 to 2 hours), highly diluted sample to avoid fast oxygen depletion in the closed chamber, and the possibility for re-oxygenations to extend measurement of oxygen flux. The mitochondrial preparation must be stable over such experimental periods of time, hence specific mitochondrial respiration media (MiR05: Gnaiger_2000_MitoInTheCold; MiR06) have been developed to maintain respiratory capacity during application of mitochondrial SUIT protocols at physiological temperature.