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- ...etting started with the [[Oroboros O2k]] to guarantee a standard [[chamber volume]] of 2.0 mL.380 bytes (49 words) - 11:57, 25 March 2022
- #REDIRECT [[O2k chamber volume calibration]]44 bytes (6 words) - 10:05, 14 August 2016
Page text matches
- ...]]'''Volume-Calibration Ring''' (A or B) PVDF for O2k-Stoppers (2-mL [[O2k-chamber]]), white; attached to the [[Stopper\black PEEK\conical Shaft\central Port |info=[https://www.oroboros.at/index.php/product/volume-calibration-ring/ '''Product details and purchase information''']1 KB (171 words) - 11:53, 11 November 2020
- ...ge:Volume calibration ring A+B PVDF for Stopper.jpg|right|180px]]'''Volume-Calibration Ring sV''' (A or B), white PVDF, attached to [[Stopper sV\black PEEK\conica |info=[https://www.oroboros.at/index.php/product/volume-calibration-ring-sv/ '''Product details and purchase information''']1 KB (184 words) - 11:25, 11 November 2020
- ...etting started with the [[Oroboros O2k]] to guarantee a standard [[chamber volume]] of 2.0 mL.380 bytes (49 words) - 11:57, 25 March 2022
- :::* Version 08 (2020-10-17) Section '''5.4. O2k-chamber volume calibration''' ::::# Instructions are added for the 0.5-mL O2k-chamber volume calibration ([[O2k-sV-Module]]).2 KB (198 words) - 11:43, 27 July 2023
- ...iological sample, and all the components necessary for a smaller operation volume ''V'' of 0.5 mL. The current DatLab version is included in the delivery of == Titration to the 0.5-mL O2k-chamber ==3 KB (426 words) - 10:05, 14 September 2023
- ...1.3 mm diameter; 50.6 mm length), [[Volume-Calibration Ring]] (A or B) for volume adjustment 1.5 to 3.2 ml; 2 mounted O-rings, with 8 spare O-rings ([[O-ring ...O2k-FluoRespirometer manual]] for calibration of the 2-mL [[O2k-chamber]] volume, which is identical for PEEK and PVDF stoppers.1 KB (177 words) - 10:43, 11 November 2020
- ...tential causes for '''different O<sub>2</sub> fluxes in the left and right chamber'''? ...experiments we get consistently higher fluxes in chamber A as compared to chamber B.2 KB (341 words) - 18:56, 6 November 2019
- ...mm diameter; 48.9 mm length), [[Volume-Calibration Ring sV]] (A or B) for volume adjustment 0.5 mL; 2 mounted O-rings ([[O-ring sV\Viton\9.5x1 mm]]). {{Template:Keywords: Chamber volume}}776 bytes (95 words) - 10:43, 18 November 2020
- ...1ePMu8&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3 '''A2''' Insert O2k Chamber] ...be/zZI7O5zAruw]] [https://youtu.be/zZI7O5zAruw '''A4''' O2k-chamber volume calibration]9 KB (1,435 words) - 12:42, 14 November 2023
- ...tube.com/watch?v=1MSsI1ePMu8&feature=youtu.be O2k-Videosupport: Insert O2k Chamber] .../watch?v=DuaEJc7FBjk&feature=youtu.be O2k-Videosupport: O2k Chamber volume calibration]2 KB (316 words) - 10:04, 29 March 2024
- ...gas phase above the aqueous phase in the 2-mL [[O2k-chamber]], during air calibration or for injection of nitrogen, argon or oxygen into the gas phase; Stopper-S {{Template:Keywords: Chamber volume}}865 bytes (114 words) - 10:33, 11 November 2020
- ...lary and conical basis; including [[Volume-Calibration Ring]] (A or B) for volume adjustment (1.5 to 3.2 mL); 2 mounted O-rings ([[O-ring\Viton\12x1 mm]]).809 bytes (111 words) - 16:27, 14 April 2021
- ...ollowing entries are entered separately for the left (A) and right (B) O2k-chamber. ...ion about sample used in each chamber. No sample is added in O<sub>2</sub> calibration experiments or upon cleaning.3 KB (481 words) - 13:35, 8 May 2024
- ...de and comments, are entered separately for the left (A) and right (B) O2k-chamber: ...d accordingly. Chamber B can be set to a different sample preparation than chamber A by selecting another option in the corresponding drop-down menu. The foll5 KB (713 words) - 15:58, 8 May 2024
- ...s plots are shown for the left chamber in Graph 1 (top), and for the right chamber in Graph 2 (below). During data acquisition, a 30 min time range is frequen === 01 Calibration show Temp ===11 KB (1,780 words) - 13:28, 21 March 2022
- ...pressure of oxygen [kPa], the aqueous solution is stirred rigorously in a chamber enclosing air at constant temperature. The concentration of oxygen, ''c''<s |info=[[MiPNet06.03 POS-calibration-SOP]], [[Oxygen calibration - DatLab]], [[Oxygen sensor test]]2 KB (263 words) - 11:01, 5 October 2020
- ...e/1MSsI1ePMu8]] [https://youtu.be/1MSsI1ePMu8 O2k-Videosupport: Insert O2k Chamber] ...zAruw]] [https://youtu.be/zZI7O5zAruw O2k-Videosupport: O2k Chamber volume calibration]5 KB (754 words) - 12:50, 7 November 2023
- ...n able to avoid any sample oxidative stress. During this procedure the O2k-chamber remains closed, decreasing disturbance of the system and allowing for quick ...can escape into the small bubble and then be extruded by fully closing the chamber. During the experiment re-oxygenations must be progressively performed in o5 KB (828 words) - 17:10, 31 January 2024
- ::# Insert O2k Chamber ::# Chamber volume calibration3 KB (435 words) - 09:58, 27 September 2023
- ...>O<sub>2</sub></sub> in the absence of sample. Finally, zero O<sub>2</sub> calibration completes the sensor test. ...ly intervals. These results confirm the instrumental limit of detection of volume-specific O<sub>2</sub> flux at ±1 pmol∙s<sup>−1</sup>∙mL<sup>−1</s4 KB (503 words) - 13:10, 4 August 2022
- |description=[[File:O2k-Chamber.jpg|right|180px]] ...with standard operation volumes (''V'') of 2.0 mL or 0.5 mL (small chamber volume in the [[O2k-sV-Module]], 12 mm inner diameter). The optical properties of12 KB (1,709 words) - 13:03, 5 February 2024
- ...www.youtube.com/watch?v=vFmHBevN8f4&feature=youtu.be NG O2k-Chamber volume calibration with Q-Sensor]<br> ....com/watch?v=GX_SvnVGXgg Addition of biological sample to the respirometer chamber]5 KB (760 words) - 13:23, 3 April 2022
- ...>O<sub>2</sub></sub> in the absence of sample. Finally, zero O<sub>2</sub> calibration completes the sensor test. ...ly intervals. These results confirm the instrumental limit of detection of volume-specific O<sub>2</sub> flux at ±1 pmol∙s<sup>−1</sup>∙mL<sup>−1</s4 KB (588 words) - 04:54, 9 March 2023
- ...L aliquot 5 mM storage solution with 2400 µL ethanol to reach 2.5 mL final volume; ::* Titration volume: in 2-µL steps using a 10 µL syringe (2 mL O2k-Chamber).5 KB (698 words) - 15:50, 16 March 2021
- ...>O<sub>2</sub></sub> in the absence of sample. Finally, zero O<sub>2</sub> calibration completes the sensor test. ...ly intervals. These results confirm the instrumental limit of detection of volume-specific O<sub>2</sub> flux at ±1 pmol∙s<sup>−1</sup>∙mL<sup>−1</s5 KB (653 words) - 09:12, 27 March 2024
- ...3.508 mg of Safranin and dissolve in distilled water to reach 10 mL final volume; ::::* Titration volume: in 1µL steps using a 10 µL syringe (2 mL O2k-chamber).13 KB (1,842 words) - 17:09, 17 January 2024
- ...ignal can be changed, Flux/Slope can be chosen to be displayed as Flux per volume or as normalized specific flux/flow, the background correction can be switc ...two sections are for O2k chamber A, the two section on the bottom for O2k chamber B, in each case the first row is for the signal, the second for Flux/Slope.5 KB (769 words) - 15:28, 11 April 2018
- ::1. O2k-chamber assembly ::4. Volume calibration6 KB (752 words) - 12:53, 7 November 2023
- ...gh-resolution respirometry with the calibration routine using instrumental calibration DL-Protocols in [[DatLab]]. |info=[[MiPNet06.03 POS-calibration-SOP]], [[MiPNet12.20_O2k-calibration_tutorial]]26 KB (3,995 words) - 10:15, 7 October 2021
- ...>/dt to determine oxygen consumption by the POS and backdiffusion into the chamber [1]. We evaluated instrumental tests of 48 Oroboros O2k chambers obtained f Stability of oxygen calibration signals at air saturation and zero oxygen was monitored up to 8 months. The3 KB (419 words) - 15:24, 30 May 2022
- * Total volume of [[solution]] = 1 litre. ::7) Add H<sub>2</sub>0 to a final volume of 1000 mL.8 KB (1,272 words) - 10:25, 16 March 2022
- |info=[[MiPNet06.03 POS-calibration-SOP]] ...implemented in the oxygen calibration algorithms in HRR ([[MiPNet06.03 POS-calibration-SOP]]).12 KB (1,907 words) - 10:24, 27 October 2022
- ...r [[Oxygen calibration - DatLab |calibration of oxygen sensors]] at [[Zero calibration | zero oxygen concentration]], or for stepwise reduction of oxygen [[concen |info=[[MiPNet06.03 POS-calibration-SOP]], [[MiPNet14.06 Instrumental O2 background]]9 KB (1,336 words) - 07:33, 13 October 2023
- ...|right|link=Tube Racks|thumb|'''Figure 1.''' Stoppers removed from the O2k-chamber are placed into dry Falcon tubes on the Tube Rack preventing contamination. ...nical details of the procedures used when adding cell suspensions into the chamber with stopper off and removed ('''Figure 1'''), as summarized in the followi35 KB (5,607 words) - 16:01, 21 February 2024
- ...aliquot of the 5 mM storage solution with 800 µL DMSO to reach 1 mL final volume; ...aliquot of the 5 mM storage solution with 960 µL DMSO to reach 1 mL final volume;17 KB (2,405 words) - 07:29, 5 April 2024
- ...on, enter O<sub>2</sub> solubility factor; use DatLab-Excel template '''O2-calibration.xlsx'''. ::::# O2k quality control 2: evaluate O2k chamber test; DatLab analysis of a file obtained in an instrumental O<sub>2</sub> b6 KB (767 words) - 16:54, 29 August 2022
- Yes, oxygen signals obtained after proper calibration are independent of the gain setting, and can therefore be grouped together ...ex UltraRed]] assay, when should the background H<sub>2</sub>O<sub>2</sub> calibration be performed?27 KB (4,026 words) - 11:53, 29 March 2021
- ...when receiving support on instrumental setup, including O2k chamber-volume calibration and service of the OroboPOS.3 KB (388 words) - 08:30, 21 October 2020
- ...is the name of the pull-down menu for (1) normalization of flux (chamber volume-specific flux, sample-specific flux or flow, or flux control ratios), (2) [ ...be sure to use the standard Layout 04a (Flux per volume) or 04b (Flux per volume overlay). When starting with the instrumental standard Layouts 1-3, which d17 KB (2,751 words) - 09:55, 17 November 2023
- ...POS]], and oxygen diffusion into or out of the aqueous medium in the [[O2k-chamber]]. It is a property of the instrumental system, measured in the range of ex == O<sub>2</sub> slope in the closed and open O2k-chamber ==22 KB (3,238 words) - 15:29, 2 May 2024
- ...6) via the Layout drop-down menu. Changing the normalization of flux from volume-specific flux to sample-specific flux or flow, or flux control ratios in th ...on contained in the [[O2_calibration_-_DatLab#Air_calibration_in_DatLab|O2 calibration]] window and on parameters used for [[Instrumental_background_oxygen_flux|I6 KB (916 words) - 20:54, 11 June 2023
- ==== Calibration and background source ==== ...ysis are linked to DL-Protocols. A specific DL-Protocol can be assigned to Chamber A or B, or both.10 KB (1,339 words) - 09:33, 6 May 2021
- :::: » O2k-SOP: [[MiPNet08.16 pH calibration]] ...tive) time derivative of oxygen concentration measured in a closed system, volume-specific [[proton flux]] is the time derivative of proton concentration, ex37 KB (5,713 words) - 12:13, 28 February 2023
- ...ope is calculated for the current data point. 40 is used for O<sub>2</sub> calibration and instrumental O<sub>2</sub> background tests. 20 is recommended for data ...sample are not stable, (''2'') reversible oxygen limitation in the closed chamber, (''3'') an overshoot directly after re-oxygenation, or (''4'') a slow resp4 KB (640 words) - 11:46, 25 March 2022
- :::: Titration into 2 mL O2k-chamber: '''2 µL of 10 mM AmR stock solution''', final concentration of '''10 µM ...ment (check by initial and final H<sub>2</sub>O<sub>2</sub> titrations for calibration). The ''final'' concentration of AmR becomes diminished during an experimen35 KB (5,265 words) - 15:10, 25 April 2024
- ...g solution 1''' (dissolved in 100 mM KCl solution) for use in '''2-mL O2k-chamber''': ...g solution 2''' (dissolved in 100 mM KCl solution) for use in '''2-mL O2k-chamber''':37 KB (5,710 words) - 15:30, 16 May 2023
- ...lux is a size-independent quantity where the rate is expressed per mass or volume of sample. <br> ...k-sV-Module provides all the components necessary for a smaller operation volume (0.5 mL) whereby the sample amount used for measuring oxygen flux can be re21 KB (2,889 words) - 13:31, 19 January 2021
- ...sub>2</sub> calibrations throughout the measurement. After the initial AmR calibration, ensure that the sensitivity is within the recommended range.'''<br> ...orm a test to evaluate whether this is related to an improperly positioned chamber or a faulty connection between the OroboPOS and the OroboPOS-Connector. <br29 KB (4,269 words) - 09:03, 13 May 2024
- Yes, oxygen signals obtained after proper calibration are independent of the gain setting, and can therefore be grouped together |''' Background H<sub>2</sub>O<sub>2</sub> calibration in the Amplex® UltraRed (AmR) assay:'''<br>126 KB (17,785 words) - 08:55, 13 August 2021
- ...nd if linear, the absolute mtMP can be calculated. TPP<sup>+</sup> enables calibration for measurement of mtMP.<br> Every time you exchange the chambers, a volume calibration and instrumental O2 background test should be performed. <br>85 KB (11,819 words) - 14:18, 10 January 2022
- |'''How often should I re-assemble the O2k-chamber?'''<br> ...tein contamination, medium leakage from the chamber, an oxygen leak of the chamber, or an unstable or unexpectedly high or low oxygen signal.80 KB (11,165 words) - 09:37, 8 January 2024
