SUIT protocol library: Difference between revisions
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=== GM+D+S+F+Rot === | === GM+D+S+F+Rot === | ||
# GM+D+c+S+F+Rot: | '''# GM+D+c+S+F+Rot:''' | ||
# GM+D+S+c+F+Rot: | '''# GM+D+S+c+F+Rot:''' | ||
* Cyt c: added after ADP ([[Boushel 2007 Diabetologia]]); cyt c added after S | |||
* ADP conc: | * ADP conc: | ||
* ROX: Ama ([[Boushel 2007 Diabetologia]]) | * ROX: Ama ([[Boushel 2007 Diabetologia]]) | ||
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=== GM+D+P+S+F+Rot === | === GM+D+P+S+F+Rot === | ||
# ''Innsbruck protocol 1'': GM+D+c+P+S+F+Rot: | # '''''Innsbruck protocol 1'': GM+D+c+P+S+F+Rot:''' | ||
* Respiratory states: CI(L) - CI(P) - CI+II(P) - CI+II(E) - CII(E) - ROX | * Respiratory states: CI(L) - CI(P) - CI+II(P) - CI+II(E) - CII(E) - ROX | ||
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=== PM+D+G+S+F+Rot === | === PM+D+G+S+F+Rot === | ||
# ''Innsbruck protocol 2'': PM+D+c+G+S+F+Rot | # '''''Innsbruck protocol 2'': PM+D+c+G+S+F+Rot ''' | ||
* Respiratory states: CI(L) - CI(P) - CI+II(P) - CI+II(E) - CII(E) - ROX | * Respiratory states: CI(L) - CI(P) - CI+II(P) - CI+II(E) - CII(E) - ROX | ||
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=== PMG+D+S+F+Rot === | === PMG+D+S+F+Rot === | ||
# PMG+D+c+S+F+Rot | # '''PMG+D+c+S+F+Rot''' | ||
* Respiratory states: CI(L) - CI(P) - CI+II(P) - CI+II(E) - CII(E) | * Respiratory states: CI(L) - CI(P) - CI+II(P) - CI+II(E) - CII(E) | ||
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== ETF - CI - CI+CII - CII== | == ETF - CI - CI+CII - CII== | ||
#'' Greenland-Monte Rosa protocol'': OctM+D+G+S+Rot+Omy+F | #''''' Greenland-Monte Rosa protocol'': OctM+D+G+S+Rot+Omy+F''' | ||
* Respiratory states: ETF(L) - ETF(P) - CI(P) - CI+II(P) - CII(P) - CII(L) - CII(E) - ROX | * Respiratory states: ETF(L) - ETF(P) - CI(P) - CI+II(P) - CII(P) - CII(L) - CII(E) - ROX | ||
Revision as of 14:11, 29 September 2011
CI
CII
CI+CII
CI - CI+CII - CII
GM+D+S+F+Rot
# GM+D+c+S+F+Rot: # GM+D+S+c+F+Rot:
- Cyt c: added after ADP (Boushel 2007 Diabetologia); cyt c added after S
- ADP conc:
- ROX: Ama (Boushel 2007 Diabetologia)
GM+D+P+S+F+Rot
- Innsbruck protocol 1: GM+D+c+P+S+F+Rot:
- Respiratory states: CI(L) - CI(P) - CI+II(P) - CI+II(E) - CII(E) - ROX
- Cyt c: early addition of c ensures comparability of all states in case of any c effect
- (Gnaiger_2007_MitoPathways)
PM+D+G+S+F+Rot
- Innsbruck protocol 2: PM+D+c+G+S+F+Rot
- Respiratory states: CI(L) - CI(P) - CI+II(P) - CI+II(E) - CII(E) - ROX
- (Gnaiger_2007_MitoPathways)
PMG+D+S+F+Rot
- PMG+D+c+S+F+Rot
- Respiratory states: CI(L) - CI(P) - CI+II(P) - CI+II(E) - CII(E)
- ADP conc: in mouse brain homogenate a final conc. of 2 mM ADP in the OXPHOS state (CI+CII) is saturating (Sumbalova_2011_Abstract)
- ROX: Mna+Ama; Myx (Gnaiger_2009_IJBCB)
ETF - CI
ETF - CI+CII
ETF - CI - CI+CII - CII
- Greenland-Monte Rosa protocol: OctM+D+G+S+Rot+Omy+F
- Respiratory states: ETF(L) - ETF(P) - CI(P) - CI+II(P) - CII(P) - CII(L) - CII(E) - ROX
- Cyt c: added after F. The absence of a significant c-effect shows the integrity of the outer mitochondrial membrane after fibre preparation and also shows the preservation of integrity over a 90-100 min incubation in the O2k (MiR06).
- Oxygen: oxygen limitation of flux was preventd by maintaining oxygen levels in the respirometer above air saturation (Pesta_2011_Protocols)
- ROX: Mna+Ama
- (Gnaiger_2007_MitoPathways)
