SUIT-016

high-resolution terminology - matching measurements at high-resolution

SUIT-016

Description

File:FNS 1OctM 2D 3G 4S 5Rot 6Omy 7U 8c 9Ama.jpg

SUIT-catg: FNS

Abbreviation: FNS_1OctM 2D 3G 4S 5Rot 6Omy 7U 8c 9Ama

Reference: Gnaiger 2015 Scand J Med Sci Sports

MitoPedia concepts: SUIT protocol 

Subcatg: F+FN+FNS+S

SUIT protocol pattern: diametral

Mark names in DatLab: Gnaiger_2015_Scand_J_Med_Sci_Sports

DatLab-Excel template: in prep.

SUIT states: 1-2OctM(LP) 3GMOct 4GMSOct 5-8S(PLEc) 9ROX

Step	Respiratory state	Pathway control	Pathway to Q	Comment
1OctM	OctM(L)	F	CETF
2D	OctM(P)	F	CETF	OXPHOS coupling efficiency in state F.
3G	GMOct(P)	FN	FAO&CI	F/N ratio or (N-F)/N flux control factor of OXPHOS capacity.
4S	GMSOct(P)	FNS	FAO&CI&II	Additive effect of the NS pathway combination, compared to the sum of N+S pathway fluxes measured separately, in the OXPHOS state and the presence of F.
5Rot	S(P)	S	CII	The P/E ratio is not obtained in the FNS state, with preference given to the next steps for OXPHOS coupling efficiency. A succinate concentration of >10 mM may be required for saturating SP capacity.
6Omy	S(L)	S	CII	OXPHOS coupling efficiency in state S, comparable to state F.
7U	S(E)	S	CII	The complete ETS capacity in state S may not be obtained after oligomycin. If apparent P/E>1.0, this is corrected to 1.0.
8c	S(E)	S	CII	If cytochrome c control factors = 0.0, the cytochrome c test conducted at this very late ETS state provides an additional indication of stability of the mt-preparation during the experimental assay. Application of the cytochrome c test earlier in the protocol (SUIT_NS_GM_01: 1GM 2D 3c 4S ..) is preferable, if cytochrome c control factors > 0.0 may be observed occasionally or regulary. If such cytochrome c effects are not exclusion criteria, then OXPHOS states with all substrate combinations can be compared in the presence of cytochrome c.
9Ama		ROX	ROX	ROX may be lower in substrate states earlier in the SUIT protocol. Therefore, this ROX measurement is frequently taken as a methodological control rather than as the basis of ROX correction of mitochondrial respiration (mt).