Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "O2k-FluoRespirometer"

From Bioblast
Line 67: Line 67:


::::* O2k '''net weight is reduced''' to 13.5 kg in comparison to 21 kg in Series D.
::::* O2k '''net weight is reduced''' to 13.5 kg in comparison to 21 kg in Series D.
== The '''O2k-FluoRespirometer''' ''consists of''==
{{#ask: [[Category:Products]] [[Product type::O2k-FluoRespirometer]]
| ?description
| ?product id
| ?product image
|sort=product id
|order=ascending
|mainlabel=Title
}}
== Electric and lab space requirements ==
::::* Max. power input: 120 W
::::*Current at 100-120 V: max. 1.0 A
::::*Current at 220-240 V: max. 0.5 A
::::*Power consumption during standard operation (when block temperature is stable at 37 Β°C and ambient temperature of 22 Β°C): approx. 24 W
:::: The O2k requires a minimum of lab space (0.5 m bench space) next to an external PC or laptop, with sufficient space for the ISS, Titration Set and handling.
:::: For maximum signal stability and optimal temperature control, the O2k should be placed in a position in which it is not exposed to direct sunlight and sudden changes of room temperature. Keep the space on the table below the O2k bottom clear to prevent any interference of ventilation beneath the O2k-Main Unit.
== Further information and references ==
::::Β» For offers please contact: [mailto:[email protected]| [email protected]]
::::Β» [[Oroboros Events]]
::::Β» [[O2k-Demo_experiments |O2k-Demo experiments]]
::::Β» [[MitoPedia: Fluorometry]]
[[Image:O2k-Publications.jpg|left|116px|link=O2k-Publications: Topics|O2k-Publications in the MiPMap]]
'''Β»[[O2k-Publications: Topics]]'''
'''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''.
Default sorting: chronological. Empty fields appear first in ascending order.
{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Instrument and method::O2k-Fluorometer]]
|?Was published in year
|?Has title
|?Mammal and model=Organism
|?Tissue and cell=Tissue;cell
|format=broadtable
|limit=5000
|offset=0
|sort=Was published in year
|order=descending
}}
[[Image:O2k-Publications.png|left|115px|link=O2k-Publications: Topics|O2k-Publications in the MiPMap]]
<big>'''O2k-Publications: O2k-FluoRespirometry - Abstracts'''</big>
'''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''.
{{#ask:[[Category:Abstracts]] [[Instrument and method::Oxygraph-2k]] [[Instrument and method::O2k-Fluorometer]]
|?Was submitted in year
|?Has title
|?Mammal and model=Organism
|?Tissue and cell=Tissue;cell
|format=broadtable
|limit=5000
|offset=0
|sort=Was submitted in year
|order=descending
}}
{{MitoPedia methods
|mitopedia method=Respirometry, Fluorometry
}}
{{MitoPedia O2k and high-resolution respirometry
|mitopedia O2k and high-resolution respirometry=O2k hardware
}}
{{Labeling
|instruments=O2k-Fluorometer
}}

Revision as of 14:34, 17 June 2019

                



O2k-FluoRespirometer

O2k-Catalogue

Description The Oroboros O2k-FluoRespirometer (O2k-Series H) - the experimental system complete for high-resolution respirometry (HRR), including fluorometry and the TIP2k, allowing simultaneous monitoring of oxygen consumption together with either ROS production (AmR), mt-membrane potential (TMRM, Safranin and Rhodamine 123), Ca2+ (CaG) or ATP production (MgG).

The O2k-FluoRespirometer supports all add-on O2k-Modules: O2k-TPP+ ISE-Module, O2k-pH ISE-Module, O2k-NO Amp-Module, enabling measurement of mt-membrane potential with ion sensitive electrode (ISE for TPP+ or TPMP+ ) or pH.


Parameters:

  • oxygen consumption
  • ROS production
  • ATP production
  • Ca2+
  • pH
  • mitochondrial membrane potential


Features of O2k-Series H:

  • Fluo-Control Unit integrated into O2k-Main Unit.
  • Smart Fluo-Sensors with pre-calibrated light intensities for direct input into DatLab 7
  • Added: spare OroboPOS
  • DL-Protocols as real-time guides for titrations in DatLab 7, context-sensitive help. Further details Β» MitoPedia: DatLab.
  • MiR05-Kit


The O2k is a sole source apparatus with no other instruments meeting its specifications.

Product ID 10002-02
Type O2k
Link Oroboros O2k, O2k-FluoRespirometer manual
Image
O2k-FluoRespirometer
O2k-FluoRespirometer
The O2k-FluoRespirometer[1] does not include, but fully supports add-on O2k-Modules: O2k-TPP+ ISE-Module, O2k-pH ISE-Module, O2k-NO Amp-Module.
  1. ↑ A PC is required (not included), connecting DatLab to the O2k via a USB port. For PC requirements, see here.

O2k-Series H: innovations

Publications in the MiPMap
About Oroboros
O2k-Series H: innovations


Oroboros (2017-07-11) Mitochondr Physiol Network

Abstract: MΓ©szΓ‘ros A, Krumschnabel G, Gradl P, Schwaninger H, Gnaiger E (2017) O2k-Series H: innovations. Mitochondr Physiol Network 22.09

O2k-Series H is the latest version of the Oroboros O2k for high-resolution respirometry and O2k-MultiSensor applications. The O2k-Series H sets a new standard in merging high-end technology and corporate social responsibility.


β€’ O2k-Network Lab: AT_Innsbruck_Oroboros


Features: integrated FluoRespirometry

  • The Fluorescence-control unit is integrated into the O2k-Main Unit. The new O2k-Window Frame is optimized for fixation of the Fluorescence-Sensors. The previous Window Tool, Front Fixation and Sensor-Guide are not required.
  • Smart Fluo-Sensors are pre-calibrated with sensor-specific memory and direct input into DatLab 7. O2k-Series A to G do not support the Smart Fluo-Sensors.


New: DatLab 7

Electronic innovations

  • High-resolution A-D converter: The new 24 bit analogue-digital converters provide a higher precision in measurement of O2, Amp-signals (amperometric fluorometry and nitric oxide measurements), and pX-signals (potentiometric measurements).
  • Core Relative Humidity and Core Relative Temperature: In addition to the built-in ambient temperature sensor, two new measurement channels are implemented to continuously monitor conditions within the electronic Main-Unit. Such data are especially important for quality control of experiments performed at high humidity and varying external temperature.
  • A 16 GB, industry-rated flash drive is integrated for storage of calibration files and other instrument-specific data. (This function will be available upon release of a later version of DatLab).
  • USB 2.0 Hi-Speed hub (4 external ports) for additional USB devices.
  • Three internal USB ports allow further upgrades and upwards compatibility with new developments.
  • Increased energy efficiency based on the new O2k-electronics design: power consumption is 24 W at 37 Β°C (80% reduction compared to Series D). Running costs are substantially lower as a result of our general concept for sustainable technology. The maximal power input is 120 W.
  • O2k net weight is reduced to 13.5 kg in comparison to 21 kg in Series D.