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Bioblast Agenda - 2024 week 48
2024 Week 48
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MiR05 or MiR06?
MiR06 is MiR05 plus catalase. Including catalase in the medium allows for reoxygenation with H2O2 without needing to open the chamber, and it is recommended when measuring CIV activity (with ascorbate/TMPD).
- »Catalase«; »MiR05«; »MiR06«
- communicated by »Baglivo Eleonora« and »Leo Elettra«
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From MitoFit Preprint to journal publication:
"Although FAO [fatty acid oxidation] is not a dominant pathway in brain energy metabolism, it plays important roles, such as avoiding the accumulation of long-chain acylcarnitines, and deserves specific attention in studies of brain metabolism."
- Cardoso LHD, Cecatto C, Ozola M, Korzh S, Zvejniece L, Gukalova B, Doerrier C, Dambrova M, Makrecka-Kuka M, Gnaiger E, Liepinsh E (2025) Fatty acid β-oxidation in brain mitochondria: Insights from high-resolution respirometry in mouse, rat and Drosophila brain, ischemia and aging models. »Bioblast link«
- from the O2k-Network »AT Innsbruck Oroboros« and »LV Riga Liepins E«
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Next Week
2024 Week 49
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"[...] high O2 regime (400-250 μM) and a buffer composition that do not interfere with the measurements, such as [...] MiR05, are the optimal conditions to assess mitochondrial respirometry in human skeletal muscle pfi [permeabilized fibers]"
- Doerrier C, Gama-Perez P, Pesta D, Distefano G, Soendergaard SD, Maise Chroeis K, Gonzalez-Franquesa A, Goodpaster BH, Prats C, Sales-Pardo M, Guimera R, Coen PM, Gnaiger E, Larsen S, Garcia-Roves PM (2024) Harmonization of experimental procedures to assess mitochondrial respiration in human permeabilized skeletal muscle fibers. »Bioblast link«
- from the O2k-Network »ES Barcelona Garcia-Roves PM«, »DK Copenhagen Larsen S«, »US FL Orlando Goodpaster BH«, »AT Innsbruck Oroboros«
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In focus
Stay alert
Oroboros of the month
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- In the artwork "First Fruits of the Earth Offered to Saturn" at the Palazzo Vecchio, Saturn (Cronus in Greek mythology) is holding the Ouroboros in the hand, symbolizing the eternal cycle of renewal and the interconnectedness of beginning and end.
- » Oroboros pictures of the month
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