Coupling-control state: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=''CCS'' | |abbr=''CCS'' | ||
|description='''Coupling-control states''' are defined in [[mitochondrial preparations]] (isolated mitochondria, permeabilized cells, permeabilized tissues, homogenates) as [[LEAK]], [[OXPHOS]], and [[ET pathway |ET]] states, with corresponding respiration rates (''L, P, E'') in any [[electron-transfer-pathway state]] which is competent for electron transfer. These coupling states are induced by titration of ADP and uncouplers, and application of specific inhibitors of the [[phosphorylation pathway]]. In [[living cells]], the coupling-control states are [[LEAK]], [[ROUTINE]], and [[ET pathway |ET]] states of respiration with corresponding rates ''L, R, E'', using membrane-permeable inhibitors of the [[phosphorylation system]] (e.g. [[oligomycin]]) and [[uncoupler]]s (e.g. [[CCCP]]). [[Coupling-control protocol]]s induce these coupling control states sequentially at a constant [[electron-transfer-pathway state]]. | |description='''Coupling-control states''' are defined in [[mitochondrial preparations]] (isolated mitochondria, permeabilized cells, permeabilized tissues, homogenates) as [[LEAK]], [[OXPHOS]], and [[ET-pathway |ET]] states, with corresponding respiration rates (''L, P, E'') in any [[electron-transfer-pathway state]] which is competent for electron transfer. These coupling states are induced by titration of ADP and uncouplers, and application of specific inhibitors of the [[phosphorylation pathway]]. In [[living cells]], the coupling-control states are [[LEAK]], [[ROUTINE]], and [[ET-pathway |ET]] states of respiration with corresponding rates ''L, R, E'', using membrane-permeable inhibitors of the [[phosphorylation system]] (e.g. [[oligomycin]]) and [[uncoupler]]s (e.g. [[CCCP]]). [[Coupling-control protocol]]s induce these coupling control states sequentially at a constant [[electron-transfer-pathway state]]. | ||
|info=[[BEC 2020.1]], [[Gnaiger 2009 Int J Biochem Cell Biol]] | |info=[[BEC 2020.1]], [[Gnaiger 2009 Int J Biochem Cell Biol]] | ||
}} | }} | ||
[[File:EPL-free and excess.jpg|right|300px|thumb|[[Gnaiger 2014 MitoPathways |The Blue Book 2014: Fig. 2.4.]]]] | [[File:EPL-free and excess.jpg|right|300px|thumb|[[Gnaiger 2014 MitoPathways |The Blue Book 2014: Fig. 2.4.]]]] | ||
'''Coupling-control states:''' [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link=ET capacity|ET capacity]] [[Image:L.jpg|link=LEAK respiration|LEAK]] - [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]] | '''Coupling-control states:''' [[Image:P.jpg|link=OXPHOS-capacity|OXPHOS-capacity]] [[Image:R.jpg|link=ROUTINE-respiration|ROUTINE-respiration]] [[Image:E.jpg|link=ET-capacity|ET-capacity]] [[Image:L.jpg|link=LEAK-respiration|LEAK-respiration]] - [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]] | ||
__TOC__ | __TOC__ | ||
ย Communicated by [[Gnaiger E]] 2011-02-25, edited 2019-03-08. | ย Communicated by [[Gnaiger E]] 2011-02-25, edited 2019-03-08. |
Revision as of 22:26, 10 November 2020
Description
Coupling-control states are defined in mitochondrial preparations (isolated mitochondria, permeabilized cells, permeabilized tissues, homogenates) as LEAK, OXPHOS, and ET states, with corresponding respiration rates (L, P, E) in any electron-transfer-pathway state which is competent for electron transfer. These coupling states are induced by titration of ADP and uncouplers, and application of specific inhibitors of the phosphorylation pathway. In living cells, the coupling-control states are LEAK, ROUTINE, and ET states of respiration with corresponding rates L, R, E, using membrane-permeable inhibitors of the phosphorylation system (e.g. oligomycin) and uncouplers (e.g. CCCP). Coupling-control protocols induce these coupling control states sequentially at a constant electron-transfer-pathway state.
Abbreviation: CCS
Reference: BEC 2020.1, Gnaiger 2009 Int J Biochem Cell Biol
Communicated by Gnaiger E 2011-02-25, edited 2019-03-08.
SUITbrowser question: Coupling control
- Coupling-control protocols, besides other SUIT protocols, allow to analyze coupling control in living cells or mitochondrial preparations.
- Use the SUITbrowser to find the best protocol to answer this and other research questions.
References
- Bioblast links: Uncoupling - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Specific
- ยป Artefacts by single dose uncoupling
- ยป ATP synthase
- ยป CCCP
- ยป Coupling-control protocol
- ยป DNP
- ยป Dyscoupled respiration
- ยป FCCP
- ยป Is respiration uncoupled - noncoupled - dyscoupled?
- ยป Noncoupled respiration: Discussion
- ยป Uncoupler
- ยป Uncoupled respiration - see ยป Noncoupled respiration
- ยป Uncoupling proteins
- ยป Uncoupling protein 1
- ยป Uncoupler titrations - Optimum uncoupler concentration
- Specific
- Respiratory states and control ratios
- ยป Biochemical coupling efficiency
- ยป Coupling-control state
- ยป Electron-transfer-pathway state
- ยป Electron-transfer pathway
- ET capacity
- ยป E-L coupling efficiency
- ยป Flux control efficiency
- ยป Flux control ratio
- ยป LEAK-control ratio
- ยป LEAK respiration
- ยป Noncoupled respiration
- ยป OXPHOS
- ยป OXPHOS capacity; ยป State 3
- ยป OXPHOS-control ratio, P/E ratio
- ยป Respiratory acceptor control ratio
- ยป ROUTINE-control ratio
- ยป ROUTINE respiration
- ยป ROUTINE state
- ยป State 3u
- ยป State 4
- ยป Uncoupling-control ratio UCR
- Respiratory states and control ratios
- Gnaiger E et al โ MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1
- Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002
- General (alphabetical order)
- Other keyword lists
MitoPedia concepts:
MiP concept,
Respiratory state,
SUIT concept,
SUIT state,
Recommended
MitoPedia methods:
Respirometry
MitoPedia topics:
EAGLE