Description
Malonate (malonic acid) is a competitive inhibitor of Succinate dehydrogenase (Complex II).
Abbreviation: Mna
Reference: MiPNet09.12
MitoPedia topics:
Inhibitor
Labels:
Enzyme: Complex II; Succinate Dehydrogenase"Complex II; Succinate Dehydrogenase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property.
HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
Application in HRR
Mna: Malonate (Malonic acid, CH2(COOH)2); Sigma M 1296, 5 g, store at R.T.: FW = 800
Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Preparation of 2 M stock solution (dissolved in H20): Recommended to prepare fresh every day
- 1) weigh 20.8 mg of Malonic acid directly into a Eppendorf tube of appropriate volume (final volume = 100 Β΅l)
- 2) dissolve in 20 Β΅l H2O
- 3) add 70 Β΅l 5 N KOH and check pH
- 4) titrate small amounts (2 Β΅l) of 5 N KOH until you reach a pH of 6.0 (approx. 4 titration steps). A pH of 7.0 would be ideal, however the pH jumps from pH 6.0 to pH 10.0 with only very little amounts of excess KOH.
- 5) add H2O to a final volume of 100 Β΅l
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 5 Β΅l using a 10 Β΅l syringe
- Final conc. in 2 ml O2k-chamber: 5 mM