Labieniec 2011 Int J Mol Sci: Difference between revisions
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{{Publication | {{Publication | ||
|title=Labieniec M, Siewiera K, Jozwiak Z (2011) Resorcylidene aminoguanidine (RAG) improves cardiac mitochondrial bioenergetics impaired by hyperglycaemia in a model of experimental diabetes. Int J Mol Sci 12: 8013-8026. | |title=Labieniec M, Siewiera K, Jozwiak Z (2011) Resorcylidene aminoguanidine (RAG) improves cardiac mitochondrial bioenergetics impaired by hyperglycaemia in a model of experimental diabetes. Int J Mol Sci 12: 8013-8026. | ||
|info= | |info=[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3233453/?tool=pubmed PMID: 22174647 Open Access] | ||
|authors=Labieniec M, Siewiera K, Jozwiak Z | |authors=Labieniec M, Siewiera K, Jozwiak Z | ||
|year=2011 | |year=2011 | ||
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|tissues=Cardiac muscle | |tissues=Cardiac muscle | ||
|preparations=Isolated Mitochondria | |preparations=Isolated Mitochondria | ||
|topics=Respiration; OXPHOS; ETS | |topics=Respiration; OXPHOS; ETS Capacity | ||
}} | }} |
Revision as of 11:07, 3 August 2012
Labieniec M, Siewiera K, Jozwiak Z (2011) Resorcylidene aminoguanidine (RAG) improves cardiac mitochondrial bioenergetics impaired by hyperglycaemia in a model of experimental diabetes. Int J Mol Sci 12: 8013-8026. |
Labieniec M, Siewiera K, Jozwiak Z (2011) Int J Mol Sci
Abstract: Diabetes is associated with a mitochondrial dysfunction. Hyperglycaemia is also clearly recognized as the primary culprit in the pathogenesis of cardiac complications. In response to glycation and oxidative stress, cardiac mitochondria undergo cumulative alterations, often leading to heart deterioration. There is a continuous search for innovative treatment strategies for protecting the heart mitochondria from the destructive impact of diabetes. Aminoguanidine derivatives have been successfully used in animal model studies on the treatment of experimental diabetes, as well as the diabetes-driven dysfunctions of peripheral tissues and cells. Considerable attention has been paid particularly to ฮฒ-resorcylidene aminoguanidine (RAG), often shown as the efficient anti-glycation and anti-oxidant agent in both animal studies and in vitro experiments. The aim of the present study was to test the hypothesis that RAG improves oxidative phosphorylation and electron transport capacity in mitochondria impaired by hyperglycaemia. Diabetes mellitus was induced in Wistar rats by a single intraperitoneal injection of streptozotocin (70 mg/kg body weight). Heart mitochondria were isolated from healthy rats and rats with streptozotocin-diabetes. Mitochondrial respiratory capacity was measured by high resolution respirometry with the OROBOROS Oxygraph-2k according to experimental protocol including respiratory substrates and inhibitors. The results revealed that RAG protects the heart against diabetes-associated injury by improving the mitochondrial bioenergetics, thus suggesting a possible novel pharmacological strategy for cardioprotection. โข Keywords: RAG (ฮฒ-resorcylidene aminoguanidine), mitochondrial respiratory, streptozotocin diabetes, oxygraph Oroboros
โข O2k-Network Lab: PL_Lodz_Watala C
Labels:
Organism: Rat
Tissue;cell: Cardiac muscle"Cardiac muscle" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.
Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Oxygraph-2k