Three-electrode system: Difference between revisions
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|description= A '''three-electrode system''' is the setup used in the [[Q-Sensor]], which is an integral part of the [[Q-Module]]. This system is used in voltammetry (including [[cyclic voltammetry]]) to study the current as a function of the applied potential using three different electrodes:ย ย ย 1) the working electrode 2) the reference electrode, and 3) the counter electrode. | |description= A '''three-electrode system''' is the setup used in the [[Q-Sensor]], which is an integral part of the [[Q-Module]]. This system is used in voltammetry (including [[cyclic voltammetry]]) to study the current as a function of the applied potential using three different electrodes:ย ย ย 1) the working electrode 2) the reference electrode, and 3) the counter electrode. In the [[Q-Sensor]], the working or detecting electrode is a glassy carbon (GC) electrode that is set to a given potential and makes contact with the analyte. The potential of the working electrode is controlled by the constant potential of the a silver/silver chloride (Ag/AgCl) reference electrode, which does not pass any current.ย The applied potential on the surface of the GC should be sufficient to either oxidize reduced analyte (in this case [[Coenzyme Q]]) or to reduce oxidized analyte. Thus, the counter electrode is a platinum electrode (Pt) that passes a current to counter these redox events by completing the circuit that is rate-limited by electron transfer on the GC. To determine the Q redox ratio the GC electrode is set at the oxidation peak potential, which can be determined with [[cyclic voltammetry]]. | ||
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Revision as of 13:35, 16 March 2021
Description
A three-electrode system is the setup used in the Q-Sensor, which is an integral part of the Q-Module. This system is used in voltammetry (including cyclic voltammetry) to study the current as a function of the applied potential using three different electrodes: 1) the working electrode 2) the reference electrode, and 3) the counter electrode. In the Q-Sensor, the working or detecting electrode is a glassy carbon (GC) electrode that is set to a given potential and makes contact with the analyte. The potential of the working electrode is controlled by the constant potential of the a silver/silver chloride (Ag/AgCl) reference electrode, which does not pass any current. The applied potential on the surface of the GC should be sufficient to either oxidize reduced analyte (in this case Coenzyme Q) or to reduce oxidized analyte. Thus, the counter electrode is a platinum electrode (Pt) that passes a current to counter these redox events by completing the circuit that is rate-limited by electron transfer on the GC. To determine the Q redox ratio the GC electrode is set at the oxidation peak potential, which can be determined with cyclic voltammetry.
The Q-Sensor is an integral part of the Q-Module and NextGen-O2k project
- The Q-Module allows for monitoring of the redox state of electron transfer-reactive coenzyme Q at the Q-junction using the specific Q-Stoppers with the integrated three-electrode system and the modified electronics of the NextGen-O2k. Cyclic voltammetry is used for quality control and for defining the polarization voltage applied during Q-redox measurements.
- Reference:
- Komlรณdi T, Cardoso LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. Bioenerg Commun 2021.3. https://doi.org/10.26124/bec:2021-0003
- Reference:
Communicated by Komlodi T, Cardoso LHD 2020-07-28
- Bioblast links: Q - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coenzyme Q
- ยป Coenzyme Q
- ยป Quinone, Ubiquinone Q; oxidized
- ยป Quinol, Ubiquinol QH2; reduced
- ยป Semiquinone
- ยป Coenzyme Q2
- ยป Q-redox state
- ยป Q-pools
- Coenzyme Q
- Mitochondrial pathways, respiratory Complexes, and Q
- ยป Q-cycle
- ยป Q-junction
- ยป Convergent electron flow
- ยป NS-pathway
- ยป FNS
- ยป FNSGp
- Mitochondrial pathways, respiratory Complexes, and Q
- ยป N-pathway
- ยป Reverse electron flow from CII to CI
- ยป CI
- ยป Rotenone
- ยป Amytal
- ยป Piericidin
- NextGen-O2k and Q-Module
- ยป NextGen-O2k
- ยป Q-Module
- ยป Q-Sensor
- ยป Cyclic voltammetry
- ยป Three-electrode system
- NextGen-O2k and Q-Module
- General