MiPNet19.15 IOC97
 Lausanne CH, 2014 Sep. 25-26 Oroboros O2k-Workshop on HRR and O2k-Fluorometry, IOC97. |
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Oroboros (2014-09-25) Mitochondr Physiol Network
Abstract:
97th Oroboros O2k-Workshop on high-resolution respirometry and O2k-Fluorometry
âą O2k-Network Lab: CH Lausanne Sandi C, AT Innsbruck Oroboros
Labels:
HRR: Oxygraph-2k
ORO, IOC, 2014, O2k-Network
» MitoPedia: Respiratory states
Lecturer
Gnaiger Erich, CEO, Oroboros Instruments
Programme
Programme MiPNet19.15.pdf
Thursday, September 25
09:00 â 10:30 A new Power-O2k Lab of Prof. Carmen Sandi: 4 O2k.
- Instrumental setup, chamber assembly, OroboPOS â sensor service, quality control (group Carmen Sandi).
10:30 Break â Registration of external participants
11:00 â 11:15 Erich Gnaiger:
- Welcome to external participants. Oroboros Instruments and perspectives of mitochondrial physiology.
11:15 â 11:30 Introduction of participants: who is who?
11:30 â 12:30 Basic and new features of DatLab 6.
- Oxygen calibration.
- Oxygen flux and instrumental tests.
12:30 Lunch
13:30 â 15:30 Experimental design.
- Cell ergometry: from intact cells to mt-preparations.
- A challenge for simultaneous measurements of respiration and mt-membrane potential: solving a puzzle.
15:30 Break
16:00 â 17:30 Titration-Injection microPump TIP2k:
- From automatic instrumental background tests to steady-state control in respiratory experiments.
17:30 â 18:00 Q&A session on HRR and OXPHOS analysis
- Design of experimental protocol - day 2.
Friday, September 26
09:00 â 10:30 Experiment
- HRR and O2k-Fluorometry â respiration and H2O2 production.
10:30 Break
11:00 â 12:30 Experiment continued.
12:30 Lunch
13:15 â 15:30 DatLab analysis.
15:30 Break
16:00 â 16:30 Trouble shooting.
16:30 â 17:30 Normalization of mt-respiration: flux control states and flux control steps.
17:30 - 18:00 Feedback â conclusions â stay connected as a MiPNet Lab.
Venue
Laboratory of Behavioral Genetics
Brain Mind Institute
Ecole Polytechnique Federale de Lausanne
Auditorium/Meeting room
CH-1015 Lausanne
Contact: Prof. Carmen Sandi, Director. Email: [email protected]
Registration and general information
Oroboros Instruments
high-resolution respirometry
Schöpfstrasse 18
A-6020 Innsbruck, Austria
Tel: +43 512 566796
Fax: +43 512 566796 20
Participants
| Name | Institution |
|---|---|
| Canto_AC (Thursday, Friday) | CH Lausanne Canto C: Nestlé Institute of Health Sciences SA, EPFL |
| Eggimann Sandra | CH Bern Nuoffer JM: UniversitĂ€tsinstitut fĂŒr klinische Chemie, Inselspital Bern |
| Grosse Jocelyn (Thursday) | CH Lausanne Sandi C: Brain Mind Institute, EPFL |
| Hahn Dagmar | CH Bern Nuoffer JM: UniversitĂ€tsinstitut fĂŒr klinische Chemie, Inselspital Bern |
| Hollis Fiona (Thursday afternoon and Friday) | CH Lausanne Sandi C: Brain Mind Institute, EPFL |
| Huzard Damien (Thursday morning and Friday morning) | CH Lausanne Sandi C: Brain Mind Institute, EPFL |
| Meng KĂ©vin (Thursday afternoon and Friday morning) | CH Lausanne Sandi C: Brain Mind Institute, EPFL |
| Kulkarni Sameer | CH Lausanne Canto C: Nestlé Institute of Health Sciences SA, EPFL |
| Papilloud Aurélie (Thursday and Friday) | CH Lausanne Sandi C: Brain Mind Institute, EPFL |
| Ratajczak Joanna (Thursday afternoon and Friday) | CH Lausanne Canto C: Nestlé Institute of Health Sciences SA, EPFL |
| Riccio Orbicia (Thursday afternoon and Friday) | CH Lausanne Sandi C: Brain Mind Institute, EPFL |
| Sandi Carmen | CH Lausanne Sandi C: Brain Mind Institute, EPFL |
| Walker Sophie (Thursday) | CH Lausanne Sandi C: Brain Mind Institute, EPFL |
| Zanoletti Olivia (Thursday and Friday) | CH Lausanne Sandi C: Brain Mind Institute, EPFL |
Recommended reading
Key references
- Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. »Bioblast Access
- Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58. »Bioblast Access
Introduction and O2k-Core Manual
HRR and O2k-Fluorometry
- Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. »Pubmed 24862266
Mitochondrial pathways
- Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck: 80 pp. »Open Access
O2k-Workshops are listed as MitoGlobal Events.
