MiPNet12.24 IOC44

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MiPNet12.24 IOC44

Publications in the MiPMap
Oroboros Instruments

Schroecken AT, 2007 Dec 12-16. Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC44.

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Oroboros (2007-12-12) Mitochondr Physiol Network

Abstract: O2k-International course on high-resolution respirometry. Schroecken, Voralberg, Austria; 2007 December 12-16.

>> O2k-Workshop: Current dates
>> Product: Oroboros O2k, O2k-Catalogue

O2k-Network Lab: AT_Innsbruck_Oroboros


ORO, IOC, 2007 

Team and guest tutors

Erich Gnaiger Gnaiger Erich, CEO, Oroboros Instruments and Medical University of Innsbruck, D. Swarovski Research Laboratory

Fasching Mario Fasching Mario, O2k-Network Lab: Oroboros Instruments

Köfler Simone, O2k-Network Lab: Oroboros Instruments

Helene Lemieux Lemieux Hélène, O2k-Network Lab: CA Rimouski Blier PU

Scandurra Francesca M, O2k-Network Lab: Oroboros Instruments
Subarsky Patrick, O2k-Network Lab: University of Toronto
Anderson Ethan J, O2k-Network Lab: US NC Greenville Anderson EJ


Participant Institution
Gallardo Pérez Esther M SP Universidad Autonoma de Madrid (SP)
Gusdon Aaron M US FL Gainesville Mathews CE: The University of Florida College of Medicine (US)
Johnson Leif SE Lund University (SE)
Kane DA.jpg Kane Daniel A CA Antigonish Kane DA: St. Francis Xavier University (CA)
Lanza Ian R US MN Rochester Nair KS: Mayo Clinic School of Medicine (US)
Medina Miguel Angel ES Malaga Medina MA: University of Málaga (ES)
Nowak Barbara PL Krakow Dembinska-Kiec A (PL)
Pajuelo-Reguera David CZ Prague Jezek P: Academy of Sciences of the Czech Republic (CZ)
Ruiz-Perez Maria Victoria ES Malaga Medina MA: University of Málaga (ES)
Scatena Roberto IT Rome Scatena R: Policlinico A. Gemelli - Università Cattolica (IT)
Kane Constance L CA Antigonish Kane DA: St. Francis Xavier University (CA)
Wunderlich Kirsten SE Lund University (SE)
Zadeh Zahra Ezzat CA Montreal Hepple RT: Royal Victoria Hospital (CA)


As an innovation for our O2k-Courses, Mario prepared a 'Feedback Form'. We thank the participants for taking the time to fill in these forms and answer our questions. A short summary is provided below.

Rate from 1 (low level) to 5 (high level):

  • 1.a. Were your interests covered in the course? Average: 4.5 (SD 0.5)
  • 3a. Presentation of lectures in terms of content: Average: 4.4 (SD 0.5)
  • 3b. Presentation of lectures in terms of language: Average: 4.8 (SD 0.5)
  • 5. Quality of tutoring: Average: 4.9 (SD 0.4)
  • 9. Overall raging of the O2k-Course: Average: 4.9 (SD 0.4)

Balance between 1 (not enough) to 5 (too much):

  • 2. Balance between practical “hands-on” work and “theoretical” explanations: ? Average: 2.5 (SD 0.7)
  • 4. Level of tutoring during the “hands-on” work: Average: 3.5 (SD 0.8)
  • 6. Intensity of the O2k-Course: Average: 2.3 (SD 0.9)

Questions and answers

1.b. Which topics should be covered more intensively?

  • 'Specific protocols and procedures for cells and isolated mito.'
  • 'The protocols and the specifications.'
  • 'Actual protocols/experiments done in labs and what it means.'
  • 'Perhaps more of the introductory theoretical topics. It seemed like some of the participants were less familiar with this.'

7. Please list some points of the O2k-Course that may be improved:

  • 'Just and experiment with physiological level of O2 (cells or isolated mitochondria).'
  • 'Protocols / more technical things / more practical things about DatLab.'

'More about protocols, more presentations.'

  • 'I personally liked the order of the material presented, but maybe putting electrode service/chamber assembly before the experimentation.

8. Do you have any suggestions how we could improve the course?

  • 'It is difficult to have a course with both beginners and experienced O2k users. I felt that there were too many basics covered that were appropriately described in the manual. Anyone can read it and work through procedures such as cleaning and assembling the chamber, but the theory and finer points of the technique cannot be read in any manual. I suggest having distinct groups: novice vs. somewhat experienced.'
  • 'It had beed easier for me as a total O2k and mitochondria beginner to explain first, what happens with which substances. Since most people know about that a handout of the complexes and the basic protocols in the beginning (like 1 slide of Hélène's talk had helped a lot.'
  • 'Maybe one group with the sampe people during all the course who do everything together step by step.'
  • 'Maybe more presentations from some of the participants. They could be very short presentations.'
  • 'More hands-on work.'

10. DatLab Software: Features you want to see / Problems you observed:

  • 'Its great.'
  • 'None.'
  • 'Very user friendly. I don't see any problems yet.'
  • 'It is quite good and has a lot of smart features.'
  • 'More templates for various protocols.'

11. The O2k System: Features you want to see / Problems you observed:

  • 'None.'
  • 'No problem for the moment.'
  • 'Would love to do ROS measurements with it.'


  • 'Very positive to me, that I could ask the most stupid questions without feeling stupid. Great, patient team. Good, that groups and team change all the time, everybody stresses other things. Perfect size of the course. Very good how you managed to combine all different levels. I am looking forward to use the O2k and discuss results.'

Feedback from the correspondence

  • 'Thank you for great days in Schröcken - the very pleasant and fruitful introduction to the Oxygraph, to snow-shoes and to local culture, and the brain-storming about career, good and bad choices, and career-choices ..' - Ruiz-Perez Maria Victoria, O2k-Network-Lab: ES Malaga Medina MA
  • 'I have to say that the O2K course was extremely worthwhile for me, and especially for Dan. We will have much useful information and technical aids to improve our O2K measurements. You and your people were incredibly helpful and gracious hosts, and I wanted to express my appreciation for that. We have nothing but great memories to last from this experience.' - Anderson Ethan J, O2k-Network Lab: US NC Greenville Anderson EJ
  • 'Many thanks to you and your staff for the hospitality and collegiality given to Ethan and Dan. They have returned with nothing but high praise for the course and for Austria. As they are learning, one of the most rewarding aspects of a career in research is getting to meet and spend time with like-minded people from all over the world. Life is definitely enriched with every new friend you make, especially when you can do it drinking a beer you’ve never tried before! I hope to make it to the course sometime soon, perhaps next winter.' - Neufer P Darrell, O2k-Network Lab: US NC Greenville Neufer PD

Impressions from the IOC44

O2k-Workshops are listed as MitoGlobal Events.
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