Difference between revisions of "User:Spitzer Gudrun/instructions updated"
From Bioblast
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* [[Pyruvate]]: deleted the line that says that pH has to be adjusted and added comment why this is not necessary | * [[Pyruvate]]: deleted the line that says that pH has to be adjusted and added comment why this is not necessary | ||
* [[Antimycin_A]]: clearified hygroscopicity and light sensitivity | * [[Antimycin_A]]: clearified hygroscopicity and light sensitivity | ||
* [[KCN]]: corrected vendor, because product number belongs to Sigma, not Fluka | * [[KCN]]: corrected vendor, because product number belongs to Sigma, not Fluka. | ||
== ToDo == | == ToDo == | ||
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* [[Ascorbate]] 0.8M - 1M (could be volume adjusted but is not indicated in Bioblast) | * [[Ascorbate]] 0.8M - 1M (could be volume adjusted but is not indicated in Bioblast) | ||
* [[Carnitine]] infos for sV-chamber are missing | * [[Carnitine]] infos for sV-chamber are missing | ||
* [[Malonate]] volume of the solute is not taken into account | |||
Revision as of 12:25, 16 March 2021
On following pages the instructions were updated during preparation of the SUIT chemical paper 2021-01 and 2021-02
Done
- Carbonyl_cyanide_m-chlorophenyl_hydrazone. It is not recommended to store the whole stock solution in one vial. Therefore this instruction was deleted.
- ADP: adapted instructions to what we really do: Take pre-weighted ADP and prepare with MgCl2 and not without it. Refined instructions a bit: moved comments from the instructions-paragraph to the comments-paragraph
- Salicylhydroxamic_acid: Maria asked me to prepare in higher concentration. The instructions were adapted accordingly.
- CCCP: clearify the chatty discussion about DMSO as a solvent
- Pyruvate: deleted the line that says that pH has to be adjusted and added comment why this is not necessary
- Antimycin_A: clearified hygroscopicity and light sensitivity
- KCN: corrected vendor, because product number belongs to Sigma, not Fluka.
ToDo
- FCCP delete the statement that the whole stock solution can be stored in one vial. sV-chamber-instructions need update
- CCCP clear enough now?
- TMPD describe reaction to light / oxygen
- Octanoylcarnitine correct the vendors
- Ascorbate 0.8M - 1M (could be volume adjusted but is not indicated in Bioblast)
- Carnitine infos for sV-chamber are missing
- Malonate volume of the solute is not taken into account
