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Difference between revisions of "User:Spitzer Gudrun/instructions updated"

From Bioblast
Line 13: Line 13:
* [[Octanoylcarnitine]] correct the vendors
* [[Octanoylcarnitine]] correct the vendors
* [[Ascorbate]] 0.8M - 1M (could be volume adjusted but is not indicated in Bioblast)
* [[Ascorbate]] 0.8M - 1M (could be volume adjusted but is not indicated in Bioblast)
* [[Carnitine]] infos for sV-chamber are missing

Revision as of 09:45, 16 March 2021

On following pages the instructions were updated during preparation of the SUIT chemical paper 2021-01 and 2021-02

Done

  • Carbonyl_cyanide_m-chlorophenyl_hydrazone. It is not recommended to store the whole stock solution in one vial. Therefore this instruction was deleted.
  • ADP: adapted instructions to what we really do: Take pre-weighted ADP and prepare with MgCl2 and not without it. Refined instructions a bit: moved comments from the instructions-paragraph to the comments-paragraph
  • Salicylhydroxamic_acid: Maria asked me to prepare in higher concentration. The instructions were adapted accordingly.
  • CCCP: clearify the chatty discussion about DMSO as a solvent
  • Pyruvate: deleted the line that says that pH has to be adjusted and added comment why this is not necessary

ToDo

  • FCCP delete the statement that the whole stock solution can be stored in one vial. sV-chamber-instructions need update
  • TMPD describe reaction to light / oxygen
  • Octanoylcarnitine correct the vendors
  • Ascorbate 0.8M - 1M (could be volume adjusted but is not indicated in Bioblast)