Difference between revisions of "SUIT protocol library"
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* Respiratory states: ETF(L) - ETF(P) - CI(P) - CI+II(P) - CII(P) - CII(L) - CII(E) - ROX | * Respiratory states: ETF(L) - ETF(P) - CI(P) - CI+II(P) - CII(P) - CII(L) - CII(E) - ROX | ||
* [[Cytochrome c|Cyt c]]: added after F. The absence of a significant c-effect shows the integrity of the outer mitochondrial membrane after fibre preparation and also shows the preservation of integrity over a 90-100 min incubation in the O2k ([[MiR06]]). | * [[Cytochrome c|Cyt c]]: added after F. The absence of a significant c-effect shows the integrity of the outer mitochondrial membrane after fibre preparation and also shows the preservation of integrity over a 90-100 min incubation in the O2k ([[MiR06]]). | ||
* Oxygen: oxygen limitation of flux was preventd by maintaining oxygen levels in the respirometer above air saturation ([[ | * Oxygen: oxygen limitation of flux was preventd by maintaining oxygen levels in the respirometer above air saturation ([[Pesta 2012 Methods Mol. Biol.]]) | ||
* [[ROX]]: Mna+Ama | * [[ROX]]: Mna+Ama | ||
* ([[Gnaiger_2007_MitoPathways]]) | * ([[Gnaiger_2007_MitoPathways]]) | ||
Revision as of 16:31, 17 November 2011
CI
CII
CI+CII
CI - CI+CII - CII
GM+D+S+F+Rot
- Innsbruck-Copenhagen protocol: GM+D+c+S+F+Rot:
- GM+D+S+c+F+Rot:
- Cyt c: added after ADP (Boushel 2007 Diabetologia); cyt c added after S
- ADP conc:
- ROX: Ama (Boushel 2007 Diabetologia)
GM+D+P+S+F+Rot
- Innsbruck protocol 1: GM+D+c+P+S+F+Rot:
- Respiratory states: CI(L) - CI(P) - CI+II(P) - CI+II(E) - CII(E) - ROX
- Cyt c: early addition of c ensures comparability of all states in case of any c effect
- (Gnaiger_2007_MitoPathways)
PM+D+G+S+F+Rot
- Innsbruck protocol 2: PM+D+c+G+S+F+Rot
- Respiratory states: CI(L) - CI(P) - CI+II(P) - CI+II(E) - CII(E) - ROX
- (Gnaiger_2007_MitoPathways)
PMG+D+S+F+Rot
- PMG+D+c+S+F+Rot
- Respiratory states: CI(L) - CI(P) - CI+II(P) - CI+II(E) - CII(E)
- ADP conc: in mouse brain homogenate a final conc. of 2 mM ADP in the OXPHOS state (CI+CII) is saturating (Sumbalova_2011_Abstract)
- ROX: Mna+Ama; Myx (Gnaiger_2009_IJBCB)
ETF - CI
ETF - CI+CII
ETF - CI - CI+CII - CII
- Greenland-Monte Rosa protocol: OctM+D+G+S+Rot+Omy+F
- Respiratory states: ETF(L) - ETF(P) - CI(P) - CI+II(P) - CII(P) - CII(L) - CII(E) - ROX
- Cyt c: added after F. The absence of a significant c-effect shows the integrity of the outer mitochondrial membrane after fibre preparation and also shows the preservation of integrity over a 90-100 min incubation in the O2k (MiR06).
- Oxygen: oxygen limitation of flux was preventd by maintaining oxygen levels in the respirometer above air saturation (Pesta 2012 Methods Mol. Biol.)
- ROX: Mna+Ama
- (Gnaiger_2007_MitoPathways)
