SUIT-009 O2 ce-pce D016: Difference between revisions

Revision as of 15:29, 21 January 2019

high-resolution terminology - matching measurements at high-resolution

SUIT-009 O2 ce-pce D016

Abbreviation: NS(P)

Reference: A: SUIT-009 short protocol for mt-respiration in pce.

SUIT-category: NS(P)

SUIT protocol pattern: orthogonal 1S;2D;3P;4Rot;5Ama

For H₂O₂-Application, choose SUIT-009 AmR pce D019; for O2 and H₂O₂ measurements (AmR), choose: SUIT-009 AmR pce D019.
Cytochrome c test can be performed in the S-OXPHOS state.
Reasonable duration of the experiment.

- In some cell lines e.g. HEK293T, P does not have any effect because of the downregulation of the N-linked pathway.

MitoPedia concepts: SUIT protocol, SUIT A, Find

MitoPedia methods: Respirometry

@@ Line 8: / Line 8: @@
 __TOC__
+== SUIT protocols ==
+::::* [[SUIT-009 AmR mt D021]]
+::::* [[SUIT-009 AmR pce D019]]
+::::* [[SUIT-009 O2 mt D015]]
+::::* [[SUIT-009 O2 pce D016]]
 == Strengths and limitations ==
-:::*For H2O2-Application, choose [[SUIT-009 AmR pce D019]]; for O2 and H<sub>2</sub>O<sub>2</sub> measurements (AmR), choose: [[SUIT-009 AmR pce D019]].
+:::*For H<sub>2</sub>O<sub>2</sub>-Application, choose [[SUIT-009 AmR pce D019]]; for O2 and H<sub>2</sub>O<sub>2</sub> measurements (AmR), choose: [[SUIT-009 AmR pce D019]].
-:::* Cytochrome C test can be performed in the S-OXPHOS state.
+:::* Cytochrome c test can be performed in the S-OXPHOS state.
 :::* Reasonable duration of the experiment.
 :::- In some cell lines e.g. HEK293T, P does not have any effect because of the downregulation of the N-linked pathway.