Difference between revisions of "SUIT-002 O2 ce-pce D007"
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# | {{MitoPedia | ||
|application=O2 | |||
|abbr=FNSGp(PGM) | |||
|description=[[File:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot-.png|400px]] | |||
|SUIT number=D07a_ce1;1Dig;1D;2M;3Oct;3c;4M;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd | |||
|info='''A: SUIT 2 is the RP2''' - [[MiPNet21.06 SUIT RP]] | |||
}} | |||
{{MitoPedia concepts | |||
|mitopedia concept=SUIT protocol, SUIT A | |||
}} | |||
::: [[MitoPedia: SUIT]] - '''[[SUIT reference protocol]] RP2''' | |||
::: '''[[Categories of SUIT protocols|SUIT-category]]:''' FNSGp(PGM) | |||
::: '''[[SUIT protocol pattern]]:''' diametral | |||
__TOC__ | |||
== Harmonized SUIT protocols == | |||
=== RP1 and RP2 === | |||
'''SUIT RP1''' [[File:1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd.png|400px|SUIT-RP1]] | |||
'''SUIT RP2''' [[File:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd.png|500px|SUIT-RP2]] | |||
:::: [[1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-|SUIT RP1]]: 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;'''8Gp''';9Ama;'''10AsTm''';11Azd | |||
:::: '''SUIT RP2''': 1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;'''10Rot''';11Ama;'''12AsTm''';13Azd | |||
[[File:RP1&RP2.png|400px]] '''Harmonization between RP1 and RP2''' | |||
=== RP1 and RP2 in cells === | |||
RP2 can be assessed in cells after the selective plasma membrane permeabilization. Digitonin is a mild detergent that permeabilizes plasma membranes. The optimum effective digitonin concentration for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol (see [[Digitonin]]). The use of intact cells allows the possibility to evaluate respiration in two different modules: intact cells and permeabilized cells. ROUTINE respiration (ce1) is obtained before digitonin is employed, whereas after the use of digitonin we can address differentet [[Electron transfer-pathway state|Electron transfer-pathway statea]]. | |||
:::: [[1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-#Harmonized_SUIT_protocols|SUIT RP1 in cells]]: '''ce1''';1Dig;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;'''8Gp''';9Ama;'''10Tm''';11Azd | |||
:::: '''SUIT RP2 in cells''': '''ce1''';1Dig;1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;'''10Rot''';11Ama;'''12AsTm''';13Azdd | |||
[[File:RP1&RP2_pce(Dig).png|400px]] '''Harmonization between RP1 and RP2 in cells''' | |||
=== RP1 and RP2 in PBMCs and PTLs === | |||
In PBMCs and PLTs the reference protocol RP1 is performed in the absense of Oct: [[1PM;2D;3U;4G;5S;6Rot;7Gp-|SUIT 1 for PBMCs and PTLs]] | |||
:::: [[1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-#Harmonized_SUIT_protocols|SUIT RP1 in PBMCs and PTLs]]'''SUIT RP1 for PBMCs and PLTs''':1PM;2D;2c;3U;4G;5S;6Rot;'''7Gp''';8Ama;'''9AsTm''';10Azd | |||
:::: '''SUIT RP2 PBMCs and PLTs''': 1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;'''10Rot''';11Ama;'''12AsTm''';13Azd | |||
[[File:Reference protocol RP1&RP2_pce_PBMCs_PTLs.png|400px]] '''Harmonization between RP1 and RP2 in PBMC and PTL cells''' | |||
== SUIT O2 DLP for SUIT 2 (RP2) (DatLab 7.3) == | |||
''Updating this page (2018-06-13) for DatLab 7.3 DLP files'' | |||
== References == | |||
{{#ask:[[Category:Publications]] [[Additional label::1D;2OctM;3P;4G;5S;6Gp;7U;8Rot-]] | |||
|?Was published in year=Year | |||
|?Has title=Reference | |||
|?Mammal and model=Organism | |||
|?Tissue and cell=Tissue;cell | |||
|format=broadtable | |||
|limit=5000 | |||
|offset=0 | |||
|sort=Was published in year | |||
|order=descending | |||
}} |
Revision as of 15:10, 27 June 2018
Description
Abbreviation: FNSGp(PGM)
Reference: A: SUIT 2 is the RP2 - MiPNet21.06 SUIT RP
SUIT number: D07a_ce1;1Dig;1D;2M;3Oct;3c;4M;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd
O2k-Application: O2
MitoPedia concepts:
SUIT protocol,
SUIT A
- MitoPedia: SUIT - SUIT reference protocol RP2
- SUIT-category: FNSGp(PGM)
- SUIT protocol pattern: diametral
Harmonized SUIT protocols
RP1 and RP2
- SUIT RP1: 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd
- SUIT RP2: 1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd
Harmonization between RP1 and RP2
RP1 and RP2 in cells
RP2 can be assessed in cells after the selective plasma membrane permeabilization. Digitonin is a mild detergent that permeabilizes plasma membranes. The optimum effective digitonin concentration for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol (see Digitonin). The use of intact cells allows the possibility to evaluate respiration in two different modules: intact cells and permeabilized cells. ROUTINE respiration (ce1) is obtained before digitonin is employed, whereas after the use of digitonin we can address differentet Electron transfer-pathway statea.
- SUIT RP1 in cells: ce1;1Dig;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd
- SUIT RP2 in cells: ce1;1Dig;1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azdd
Harmonization between RP1 and RP2 in cells
RP1 and RP2 in PBMCs and PTLs
In PBMCs and PLTs the reference protocol RP1 is performed in the absense of Oct: SUIT 1 for PBMCs and PTLs
- SUIT RP1 in PBMCs and PTLsSUIT RP1 for PBMCs and PLTs:1PM;2D;2c;3U;4G;5S;6Rot;7Gp;8Ama;9AsTm;10Azd
- SUIT RP2 PBMCs and PLTs: 1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd
Harmonization between RP1 and RP2 in PBMC and PTL cells
SUIT O2 DLP for SUIT 2 (RP2) (DatLab 7.3)
Updating this page (2018-06-13) for DatLab 7.3 DLP files