OctPM pathway control state: Difference between revisions
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== PMOct(L) == | |||
== PMOct(P) == | |||
# [[SUIT-RP2]]_'''4P''': 1D 2Oct 3Mtit 3c (3NADH) '''<u>4P</u>''' 5G 6S 7U 8Gp 9Rot 10Ama 11Tm 12Azd''' | |||
== PMOct(E) == | |||
# [[SUIT-RP1]]_'''4Oct''': 1PM 2D 2c (2NADH) 3U '''<u>4Oct</u>''' 5G 6S 7Rot 8Gp 9Ama 10Tm 11Azd | |||
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[[File:SUIT-Reference-Protocol 1.jpg|700px |SUIT RP1]]<br /> | |||
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[[File:SUIT-Reference-Protocol 2.jpg|700px |SUIT RP2]]<br /> | |||
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Revision as of 15:16, 24 January 2016
Description
PMOct: Pyruvate & Malate & Octanoylcarnitine.
MitoPathway control: CI&FAO
SUIT protocol: SUIT-RP1, SUIT-RP1
This substrate combination supports CI-linked flux which is typically higher than FAO capacity. In SUIT-RP1, PMOct is induced after PM(E), to evaluate any additive effect of adding Oct. In SUIT-RP2, FAO OXPHOS capacity is measured first, testing for the effect of increasing malate concentration (compare Malate alone, and pyruvate is added to compare FAO as the background state with CI&FAO as the reference state.
Abbreviation: PMOct
Reference: MiPNet21.06 SUIT reference assay
MitoPedia topics: "Respiratory substrate-coupling state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. Respiratory substrate-coupling state"Respiratory substrate-coupling state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
PMOct(L)
PMOct(P)
- SUIT-RP2_4P: 1D 2Oct 3Mtit 3c (3NADH) 4P 5G 6S 7U 8Gp 9Rot 10Ama 11Tm 12Azd
PMOct(E)
- SUIT-RP1_4Oct: 1PM 2D 2c (2NADH) 3U 4Oct 5G 6S 7Rot 8Gp 9Ama 10Tm 11Azd