Difference between revisions of "MiPNet28.03 IOC157 Los Angeles US"

Latest revision as of 18:48, 1 March 2023

MiPNet28.03 IOC157 Los Angeles US

Los Angeles, CA US, 2023 Feb 14-16. Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC157.

Oroboros (2023-02-14) Mitochondr Physiol Network

Abstract:

Oroboros O2k-Workshop on high-resolution respirometry (HRR) in cooperation with Shirihai Orian. Los Angeles, US, 2023 February 14-16.

• O2k-Network Lab: US CA Los Angeles_Shirihai O, AT_Innsbruck_Oroboros

General information

Oroboros Instruments (Innsbruck, Austria) - 157^th O2k-Workshop, UCLA

Cutting-edge innovations of high-resolution respirometry (HRR) are presented by Erich Gnaiger, introducing the Oroboros NextGen-O2k [1] in parallel to hands-on training by Sabine Schmitt.

HRR targets reproducibility and flexibility of substrate-uncoupler-inhibitor titration protocols [2] for investigating mitochondrial pathway and coupling control [3]. Assays for mt-membrane potential, ROS production, ATP production, and calcium uptake are integrated in each chamber simultaneously with HRR. Modules for monitoring Q- and NADH-redox states and PhotoBiology with the NextGen-O2k open new windows for bioenergetic investigations.

NextGen-O2k all-in-one
SUIT protocols - The SUITbrowser helps you find the best SUIT protocol for your specific research questions.
Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002

Venue and organizers

UCLA Campus

650 Charles E Young Drive South Suite

Center for Health Sciences, Suite 34-115

Los Angeles, CA 90095, USA

Map

Prof. Andrea Hevener, University of California, Los Angeles, US
Prof. Orian Shirihai, University of California, Los Angeles, US
Erich Gnaiger, PhD, Oroboros Instruments - CEO, Innovation Alchemist
Sabine Schmitt, PhD, Oroboros Instruments - Mitochondrial Detective

Program

Tuesday, February 14^th CHS 27-200c - downlodad pdf

10:00-10:50am Principles of high-resolution respirometry HRR

11:00-11:50am The NextGen-O2k for Q- and NADH- redox biology

1:00-1:50pm Application of fluorescence probes with HRR for mt-phenotyping

4:00-5:00pm SuperMito Seminar

Erich Gnaiger: mitObesity - body mass excess and decline of mitochondrial fitness: from muscle to brain

Obesity is defined as accumulation of excess fat tissue mass. Compromised mitochondrial fitness across metabolically active organs provides a mechanistic connection between obesity and comorbidities: diabetes, cardiovascular and neurodegenerative diseases, and various types of cancer bound to redox imbalance, inflammation, oxidative stress, and insulin resistance.

Participants

	Participant	Institution
	Souvenir Rhonda	US CA Los Angeles_Shirihai O, UCLA, US
	Stiles Linsey	US CA Los Angeles_Shirihai O, UCLA, US
	Tran Peter	US CA Los Angeles_Shirihai O, UCLA, US
	Zhang Yuan	US CA Los Angeles_Shirihai O, UCLA, US
	Zhou Zhenqi	US CA Los Angeles_Shirihai O, UCLA, US

IOC recommended reading

» IOC recommended reading

O2k-Workshops are listed as MitoGlobal Events.'

Labels:

HRR: Oxygraph-2k

ORO, IOC, O2k-Network, 2023, MitoGlobal

@@ Line 8: / Line 8: @@
 |journal=Mitochondr Physiol Network
 |abstract=[[Image:O2k-Workshops.png|left|130px|link=O2k-Workshops]]
-'''Oroboros O2k-Workshop on high-resolution respirometry (HRR)''' in cooperation with [[Shirihai Orian]]. Los Angeles, US, 2023 February 14-16.
+'''Oroboros O2k-Workshop on high-resolution respirometry (HRR)''' in cooperation with [[US CA Los Angeles_Shirihai O |Shirihai Orian]]. Los Angeles, US, 2023 February 14-16.
-|mipnetlab=AT_Innsbruck_Oroboros,
+|mipnetlab=US CA Los Angeles_Shirihai O, AT_Innsbruck_Oroboros
 }}
 [[Image:O2k-Workshops.png|right|150px|link=http://wiki.oroboros.at/index.php/Oroboros_Events#Next_O2k-Workshops|O2k-Workshops]]
 __TOC__
+== General information ==
+[[File:IOC157 Los Angeles US team.JPG|500px|right|The IOC157 team]]
+[[File:IOC157 09.JPG|500px|right|IOC157 Lunch]]
+:::: '''Oroboros Instruments (Innsbruck, Austria) - 157<sup>th</sup> O2k-Workshop, UCLA'''
+:::: Cutting-edge innovations of '''high-resolution respirometry (HRR)''' are presented by ''Erich Gnaiger'', introducing the '''Oroboros NextGen-O2k''' [1] in parallel to hands-on training by ''Sabine Schmitt''.
+:::: HRR targets reproducibility and flexibility of '''substrate-uncoupler-inhibitor titration protocols''' [2] for investigating '''mitochondrial pathway and coupling control''' [3]. Assays for mt-membrane potential, ROS production, ATP production, and calcium uptake are integrated in each chamber simultaneously with HRR. Modules for monitoring '''Q- and NADH-redox states and PhotoBiology''' with the NextGen-O2k open new windows for bioenergetic investigations.
+::::# [https://www.oroboros.at/index.php/product/nextgen-o2k_all-in-one/ NextGen-O2k all-in-one]
+::::# [[MitoPedia:_SUIT |SUIT protocols]] - The [[SUITbrowser]] helps you find the best SUIT protocol for your specific research questions.
+::::# Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002
-== General information ==
+== Venue and organizers ==
-:::: This training course on the Oroboros [[NextGen-O2k]] provides an introduction to [[DatLab]] 8 and applications of the [[TIP2k]]. Hands-on experiments illustrate the principle and show the unique advantages and limitations of simultaneous monitoring of oxygen concentration, mitochondrial respiration and for example the Q-redox state with the newly developed [[Q-Module]]. Many optimized [[SUIT protocols]] are available as DL-Protocols. The [[SUITbrowser]] helps you find the best SUIT protocol for your specific research questions. Furthermore, this workshop covers interpretation as well as calibration and instrumental service.
-== Venue ==
 :::: UCLA Campus
 :::: 650 Charles E Young Drive South Suite
@@ Line 24: / Line 30: @@
 :::: Los Angeles, CA 90095, USA
 :::: [https://wiki.oroboros.at/images/5/55/Map_CHS_Hevener_Laboratory.pdf Map]
+<gallery mode=default perrow=4 widths="140px" heights="150px">
+File:Profile-icon-9.png|'''[[Hevener Andrea|Prof. Andrea Hevener]]''', University of California, Los Angeles, US
+File:Shirihai_O.jpg|'''[[Shirihai Orian|Prof. Orian Shirihai]]''', University of California, Los Angeles, US
+File:Erich Gnaiger.jpg|'''[[Gnaiger E |Erich Gnaiger]]''', PhD, Oroboros Instruments - CEO, ''Innovation Alchemist''
+File:SchmittS.jpg|'''[[Schmitt S|Sabine Schmitt]]''', PhD, Oroboros Instruments - ''Mitochondrial Detective''
+</gallery>
-== Local organizer ==
-:::: [[Shirihai Orian|Dr. Orian Shirihai]], University of California, Los Angeles, US
 == Program ==
-::::* Program will be uploaded shortly
+:::* Tuesday, February 14<sup>th</sup> CHS 27-200c - [https://wiki.oroboros.at/images/a/a5/2023-02-14_Oroboros_Instruments_O2k-Workshop_UCLA.pdf '''''downlodad pdf''''']
+:::: 10:00-10:50am Principles of high-resolution respirometry HRR
+:::: 11:00-11:50am The NextGen-O2k for Q- and NADH- redox biology
+:::: 1:00-1:50pm Application of fluorescence probes with HRR for mt-phenotyping
+:::: 4:00-5:00pm SuperMito Seminar
+:::::: '''Erich Gnaiger: mitObesity - body mass excess and decline of mitochondrial fitness: from muscle to brain'''
+:::::: Obesity is defined as accumulation of excess fat tissue mass. Compromised mitochondrial fitness across metabolically active organs provides a mechanistic connection between obesity and comorbidities: diabetes, cardiovascular and neurodegenerative diseases, and various types of cancer bound to redox imbalance, inflammation, oxidative stress, and insulin resistance.
-== Lecturers and tutors ==
+== Participants ==
-<gallery mode=default perrow=3 widths="140px" heights="150px">
+[[File:IOC157 05.JPG|500px|right|IOC157 Sabine introduces Oxia]]
-File:SchmittS.jpg|'''[[Schmitt S|Sabine Schmitt]]''', PhD, Oroboros Instruments - ''Mitochondrial Detective''
+[[File:IOC157 08.JPG|500px|right|IOC157 Gas injection from Oxia]]
-File:Erich Gnaiger.jpg|'''[[Gnaiger E |Erich Gnaiger]]''', PhD, Oroboros Instruments - CEO, ''Innovation Alchemist''
+{| class="wikitable"
-</gallery>
+|-
+!
+! Participant
+! Institution
+|-
+| [[File:Profile-icon-9.png|70px|Participant]]
+| [[Souvenir Rhonda ]]
+| [[US CA Los Angeles_Shirihai O]], UCLA, US
+|-
+| [[File:Profile-icon-9.png|70px|Participant]]
+| [[Stiles Linsey]]
+| [[US CA Los Angeles_Shirihai O]], UCLA, US
+|-
+| [[File:Profile-icon-9.png|70px|Participant]]
+| [[Tran Peter]]
+| [[US CA Los Angeles_Shirihai O]], UCLA, US
+|-
+| [[File:Profile-icon-9.png|70px|Participant]]
+| [[Zhang Yuan]]
+| [[US CA Los Angeles_Shirihai O]], UCLA, US
+|-
+| [[File:Profile-icon-9.png|70px|Participant]]
+| [[Zhou Zhenqi]]
+| [[US CA Los Angeles_Shirihai O]], UCLA, US
+|-
+|}
-== Participants ==
 == IOC recommended reading ==
 ::::» '''[[IOC recommended reading]]'''
-== Registration ==
-:::: will be announced shortly
 [[Image:MitoGlobal.jpg|right|80px|link=MitoGlobal|MitoGlobal]]
   O2k-Workshops are listed as [[MitoGlobal Events]].'
 {{Labeling
 |instruments=Oxygraph-2k
-|additional=ORO, IOC, O2k-Network, 2023, MitoGlobal, Next
+|additional=ORO, IOC, O2k-Network, 2023, MitoGlobal
 }}