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Innsbruck AT, 2022 Sep 19. BEC tutorial-Living Communications: pmF

MiPsociety (2022-09-19) Mitochondr Physiol Network

Abstract: BEC tutorial-Living Communications. Mitochondrial membrane potential and Peter Mitchell’s protonmotive force: elements of the science of bioenergetics.

The mitochondrial membrane potential is an element of the science of bioenergetics, linked to the control of respiratory flux and related mitochondrial functions. A PubMed search on ‘mitochondrial membrane potential’ yields nearly 40 000 results and 3442 for 2021 (search 2022-07-04), with a linear increase during the past 20 years. Chapter 8 on ‘Protonmotive pressure and respiratory control’ of Mitochondrial Pathways (Gnaiger 2020) introduces a novel perspective on Peter Mitchell’s protonmotive force, which incorporates the mitochondrial membrane potential. If you find the reading is tough, you are not alone. Join this BEC tutorial-Living Communications for an introduction into the relevant concepts of physical chemistry, which differ from misleading chapters in bioenergetics textbooks on potential gradients, Gibbs energy, protonmotive flow and force, and finally protonmotive pressure. This will introduce students (and teachers) to a new understanding of mitochondrial membrane potential and the protonmotive force, connecting the ideal gas equation, osmotic pressure, the Boltzmann constant and gas constant with Fick’s and Einstein’s diffusion equation. If theory gets tough, join for a follow-up retreat.

• O2k-Network Lab: AT_Innsbruck_Oroboros

Figure 1.1. Coupling in oxidative phosphorylation is mediated by the protonmotive force pmF.

Last update: 2022-09-15

Venue

MiPArt at Oroboros Instruments

Schöpfstr 18

6020 Innsbruck

Austria

Program

Thursday, Sep 22

Time	Section	Contents
13:00-14:30	Introduction	Protonmotive force pmF = ΔmFH+ Mitchell's theorems on four integrated coupling modules Is the protonmotive force — units [mV] or [kJ/mol] — a force? Is the "Gibbs energy" [kJ/mol] a force? Why did Peter Mitchell use the symbol Δp?
14:30-15:00	Coffee/tea	Chat
15:00-16:00	Extension	Four protonmotive theorems on protonmotive pressure ΔmΠH+ Diffusion gradients Compartments: diffusion and osmotic pressure Hydrogen ions and counterions Matrix volume fraction and flux-pressure linearity

» Programme: BEC tutorial-Living Communications: pmF to pmP

A discussion-oriented presentation

Erich Gnaiger (AT) presents the concept of protonmotive pressure in discussion with Jean-Pierre Mazat (FR) and participants.

Registration

• Informal, no registration fee - send Email to: [email protected]

Recommended reading

1. Mitchell P (1966) Chemiosmotic coupling in oxidative and photosynthetic phosphorylation. https://doi.org/10.1016/j.bbabio.2011.09.018
2. Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. https://doi.org/10.26124/bec:2020-0002 - Chapter 8
3. Gnaiger E (2021) The elementary unit — canonical reviewer's comments on: Bureau International des Poids et Mesures (2019) The International System of Units (SI) 9th ed. https://doi.org/10.26124/mitofit:200004.v2

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