Difference between revisions of "MiPNet25.06 IOC145 Innsbruck AT"

Revision as of 12:18, 6 December 2019

MiPNet25.06 IOC145 Innsbruck AT

Innsbruck AT, 2020 Jan 27-29. O2k-Coaching Days - Basic IOC145.

Oroboros (2020-01-20) Mitochondr Physiol Network

Abstract:

O2k-Coaching Days - Basic. Innsbruck, Austria; 2020 Jan 27-29.

• O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit

General information

O2k-Coaching Days - Basic is a training course which provides a fundamental introduction to high-resolution respirometry (HRR) with the Oroboros O2k. We will give you an overview of the O2k-FluoRespirometer, including real-time analysis with DatLab 7.4 and applications of the Titration-Injection microPump TIP2k. Hands-on sessions range from OroboPOS technical service (instrumental quality control system which is an essential component of HRR) to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols. Also the new Oroboros SUITbrowser will be presented. The SUITbrowser helps you to find the best SUIT protocol for your specific research questions. Many optimized SUIT protocols are available as DL-Protocols and will be shown at the O2k-Coaching Days.

Venue

» Oroboros O2k-Laboratory

Schoepfstrasse 18, 6020 Innsbruck

» Information on travel and venue: IOC Innsbruck

Registration

Registration closed.

Tutors and lecturers

Luiza Cardoso, PhD., Oroboros Instruments - PostDoc
Marco Di Marcello, BSc., Oroboros Instruments - Research Assistant
Carolina Doerrier, PhD. - Chief scientific officer
Luiz Felipe Garcia-Souza, PhD. student, Oroboros Instruments
Erich Gnaiger, PhD., Oroboros Instruments, CEO

Preliminary programme

Monday 27th

	Topic	Room
08:15	Welcome - Get-together: Introduction of participants and their research interests	Oroboros O2k-Laboratory
08:30	OroboPOS service and O2k instrumental setup - overview with videoclips	Oroboros O2k-Laboratory
09:15	Hands-on: OroboPOS service and O2k instrumental setup	Oroboros O2k-Laboratory
09:55	DatLab 7.4 overview	Oroboros O2k-Laboratory
10:30	Coffee
11:00	Instrumental quality control 1: oxygen calibration	Oroboros O2k-Laboratory
11:30	Hands-on: Instrumental quality control 1: oxygen calibration DL-Protocol: O2k-cleaning BeforeUse DL-Protocol: O2 calibration air	Oroboros O2k-Laboratory
12:00	Lunch break
13:00	Hands-on: Instrumental quality control 1: oxygen calibration (continuation) DL-Protocol: O2 calibration air	Oroboros O2k-Laboratory
13:30	Instrumental quality control 2: Instrumental O₂ background – overview with videoclips	Oroboros O2k-Laboratory
14:00	Hands-on:Instrumental quality control 2: Instrumental O₂ background DL-Protocol: Instrumental O2 background TiP2k	Oroboros O2k-Laboratory
15:00	Coffee
15:30-17:30	Hands-on: Instrumental quality control 1 and 2. DatLab analysis.	Oroboros O2k-Laboratory

Tuesday 28th

	Topic	Room
08:30	Hands-on: Instrumental quality control 1: oxygen calibration DL-Protocol: O2k-cleaning BeforeUse DL-Protocol: O2 calibration air	Oroboros O2k-Laboratory
09:15	Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. SUIT reference protocol: RP1&RP2	Oroboros O2k Laboratory
09:45	SUITbrowser: how to find the best SUIT protocol for your research questions.	Oroboros O2k Laboratory
10:00	Coffee
10:30	Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with the reference protocol RP1 (SUIT-001) and RP2 (SUIT-002). DL-Protocol: SUIT-001 O2 ce-pce D003.DLP DL-Protocol: SUIT-002 O2 ce-pce D007.DLP	Oroboros O2k Laboratory
11:45	Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning AfterUse	Oroboros O2k-Laboratory
12:00	Lunch break
13:00	Hands-on: O2k-cleaning after use (continuation) DL-Protocol: O2k-cleaning AfterUse	Oroboros O2k-Laboratory
13:30	DatLab 7.4 analysis: Introduction and new features	Oroboros O2k-Laboratory
15:00	Coffee
15:30-17:30	Individual DatLab analysis + summary	Oroboros O2k-Laboratory

Wednesday 29th

	Topic	Room
08:30	MitoFit proficiency test Hands-on: Instrumental quality control 1: oxygen calibration DL-Protocol: O2k-cleaning BeforeUse DL-Protocol: O2 calibration air	Oroboros O2k-Laboratory
10:00	Coffee
10:30	MitoFit proficiency test Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with the reference protocol RP1 (SUIT-001) and RP2 (SUIT-002). DL-Protocol: SUIT-001 O2 ce-pce D003.DLP DL-Protocol: SUIT-002 O2 ce-pce D007.DLP	Oroboros O2k-Laboratory
11:45	Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning AfterUse	Oroboros O2k-Laboratory
12:00	Lunch break
13:00	Hands-on: O2k-cleaning after use (continuation) DL-Protocol: O2k-cleaning AfterUse	Oroboros O2k-Laboratory
13:15	DatLab analysis	Oroboros O2k-Laboratory
14:00	O2k-Applications - overview	Oroboros O2k-Laboratory
14:30	Proficiency test: DatLab analysis summary	Oroboros O2k-Laboratory
15:00	Coffee
15:30	The Bioblast wiki, MitoFit Preprint Archives, O2k-Network and MitoEAGLE	MiPArt
16:30	Feedback & conclusions	MiPArt
17:00	Farewell activity	MiPArt

Participants

	Participant	Institution

Contact

Oroboros Instruments GmbH

High-Resolution Respirometry

Schoepfstrasse 18

A-6020 Innsbruck, Austria

Tel: +43 512 566796

Fax: +43 512 566796 20

E-Mail: [email protected]

Mitochondria and Cell Research

IOC recommended reading

» IOC recommended reading

» MitoPedia: Respiratory states

O2k-Workshops and O2k-Coaching Days are listed as MitoGlobal Events.

Labels:

ORO, IOC, MitoGlobal, Next, 2020

@@ Line 8: / Line 8: @@
 |journal=Mitochondr Physiol Network
 |abstract=[[Image:O2k-Workshops.png|left|130px|link=O2k-Workshops]]
-'''O2k-Coaching Days - Basic'''. Innsbruck, Austria; 2020 Jan 20-22.
+'''O2k-Coaching Days - Basic'''. Innsbruck, Austria; 2020 Jan 27-29.
 |mipnetlab=AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit
 }}
@@ Line 17: / Line 17: @@
 == General information ==
-:::: ''' The course is fully booked. In case of cancellations further registrations can be accepted. In order to be added to the waiting list, send an email to [mailto:[email protected] [email protected]]'''
 :::: '''O2k-Coaching Days - Basic''' is a training course which provides a fundamental introduction to high-resolution respirometry (HRR) with the Oroboros O2k. We will give you an overview of the O2k-FluoRespirometer, including real-time analysis with DatLab 7.4 and applications of the Titration-Injection microPump TIP2k. Hands-on sessions range from OroboPOS technical service (instrumental quality control system which is an essential component of HRR) to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols. Also the new Oroboros SUITbrowser will be presented. The SUITbrowser helps you to find the best SUIT protocol for your specific research questions. Many optimized SUIT protocols are available as DL-Protocols and will be shown at the O2k-Coaching Days.
@@ Line 25: / Line 24: @@
 ::::» Oroboros O2k-Laboratory
 ::::: Schoepfstrasse 18, 6020 Innsbruck
-::::» Information on travel and venue: '''[[IOC Innsbruck]]'''.
+::::» Information on travel and venue: '''[[IOC Innsbruck]]'''
-::::» Hotel recommendation: '''Basic Hotel Innsbruck***'''(https://www.basic-hotel.at/en/)
-::::: Using code "''OROBOROS''" for booking online you will get the Single Room for € 85.00 and the Double Room for € 120.00 including breakfast. Rooms are available at a first come first served basis.
-::::: Free cancellation until 7 days prior. Booking requires a credit card for guarantee. Payment on site. A 2 € City tax to be charged additionally.
 == Registration ==
@@ Line 54: / Line 50: @@
 <br>
 <div class="toccolours mw-collapsible mw-collapsed">
-<span style="font-size:105%; color:#424242">'''Monday 20th'''</span>
+<span style="font-size:105%; color:#424242">'''Monday 27th'''</span>
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 <br>
 <div class="toccolours mw-collapsible mw-collapsed">
-<span style="font-size:105%; color:#424242">'''Tuesday 21st'''</span>
+<span style="font-size:105%; color:#424242">'''Tuesday 28th'''</span>
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 <br>
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-<span style="font-size:105%; color:#424242">'''Wednesday 22nd'''</span>
+<span style="font-size:105%; color:#424242">'''Wednesday 29th'''</span>
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 ::::{| class='wikitable'
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 ! Participant
 ! Institution
-|-
-| [[File:Profile-icon-9.png|70px|Participant]]
-|  [[Buoso Chiara]]
-| '''AT Innsbruck Weiss G''' - Medical University Innsbruck, Innsbruck (AT)
-|-
-| [[File:BelezaJ.jpeg|100px|Participant]]
-|  [[Beleza Jorge]]
-| '''PT Porto Beleza J''' - University of Porto, Porto (PT)
 |-
-| [[File:Profile-icon-9.png|70px|Participant]]
-|  [[Carter Kristen]]
-| '''US MS Jackson Cornelius DC''' - University of Mississippi, Jackson (US)
-|-
-| [[File:Chamkha Imen.jpg|70px|Chamkha Imen]]
-|  [[Chamkha Imen]]
-| '''[[SE Lund Elmer E]]''' - Lund University, Lund (SE)
-|-
-| [[File:Profile-icon-9.png|70px|Participant]]
-|  [[Coldewey Sina]]
-| '''DE Jena Coldewey S''' - Jena University Hospital, Jena (DE)
-|-
-| [[File:Profile-icon-9.png|70px|Participant]]
-|  [[Esper Treml Ricardo]]
-| '''DE Jena Coldewey S''' - Jena University Hospital, Jena (DE)
-|-
-| [[File:Profile-icon-9.png|70px|Participant]]
-|  [[Gayathri Narayanappa]]
-| '''IN Bangalore Gayathri N''' - NIMHANS, Bangalore (IN)
-|-
-|[[File:Griffiths_Kate.JPG|60px|Kate Griffiths]]
-|  [[Griffiths Kate]]
-| '''UK Glasgow Selman C''' - Glasgow University, Glasgow (UK)
-|-
-| [[File:Profile-icon-9.png|70px|Participant]]
-|  [[Hussein Rawya]]
-| '''[[SE Lund Elmer E]]''' - Lund University, Lund (SE)
-|-
-| [[File:Profile-icon-9.png|70px|Participant]]
-|  [[Linzi Jin]]
-| '''[[CN Beijing Huawei]]''' - Huawei, Beijing (CN)
-|-
-| [[File:Profile-icon-9.png|70px|Participant]]
-|  [[Neu Charles]]
-| '''DE Jena Coldewey S''' - Jena University Hospital, Jena (DE)
-|-
-| [[File:PereiraR.jpeg|90px|Rita Pereira]]
-|  [[Pereira Rita]]
-| '''PT Porto Beleza J''' - University of Porto, Porto (PT)
-|-
-| [[File:Profile-icon-9.png|70px|Participant]]
-|  [[Place Nicolas]]
-| '''[[CH Lausanne Place N]]''' - Université de Lausanne, Lausanne (CH)
-|-
-| [[File:Profile-icon-9.png|70px|Participant]]
-|  Participant
-| '''[[SE Lund Elmer E]]''' - Lund University, Lund (SE)
 |-