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Difference between revisions of "MiPNet25.03 IOC144 Innsbruck AT"

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{{Publication
{{Publication
|title=[[Image:OROBOROS-solo.png|right|60px|link=OROBOROS INSTRUMENTS]]
|title=[[Image:OROBOROS-solo.png|right|60px|link=OROBOROS INSTRUMENTS]]
'''Innsbruck AT''', 2020 Jan 20-22. MitoFit Coaching Days - Basic '''IOC'''.
 
'''Innsbruck AT''', 2020 Jan 20-22. O2k-Coaching Days - Basic '''IOC144'''.
 
|info=[[File:PDF.jpg|100px|link=https://wiki.oroboros.at/images/1/1f/MiPNet25.03_IOC144_O2k-Coaching_Days_v2.pdf |Bioblast pdf]]
 
|authors=Oroboros
|authors=Oroboros
|year=2020-01-20
|year=2020-01-20
|journal=Mitochondr Physiol Network
|journal=Mitochondr Physiol Network
|abstract=[[Image:O2k-Workshops.png|left|130px|link=O2k-Workshops]]
|abstract=[[Image:O2k-Workshops.png|left|100px|link=O2k-Workshops]]
'''MitoFit Coaching Days - Basic'''. Innsbruck, Austria; 2020 Jan 20-22.
'''O2k-Coaching Days - Basic'''. Innsbruck, Austria; 2020 Jan 20-22.
|mipnetlab=AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit
|mipnetlab=AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit
}}
}}
'''this event is in the planning phase'''


[[Image:O2k-Workshops.png|right|150px|link=http://wiki.oroboros.at/index.php/Oroboros_Events#Next_O2k-Workshops|O2k-Workshops]]
__TOC__
__TOC__


== General information ==
== General information ==
:::: '''MitoFit Coaching Days - Basic''' is an introductory training course which provides a fundamental introduction to high-resolution respirometry (HRR) with the Oroboros O2k. We will provide an overview of the O2k-FluoRespirometer, including real-time analysis with DatLab 7.4 and applications of the Titration-Injection microPump TIP2k. <br> Hands-on sessions range from OroboPOS technical service (instrumental quality control system which is a fundamental component of HRR) to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols. Also the new Oroboros SUITbrowser will be presented. The SUITbrowser helps you to find the best SUIT protocol for your specific research questions. Many optimized SUIT protocols are available as DL-Protocols adn will be shown at the Coaching Days.
:::: ''' The course is over.
 
:::: '''O2k-Coaching Days - Basic''' is a training course which provides a fundamental introduction to high-resolution respirometry (HRR) with the Oroboros O2k. We will give you an overview of the O2k-FluoRespirometer, including real-time analysis with DatLab 7.4 and applications of the Titration-Injection microPump TIP2k. Hands-on sessions range from OroboPOS technical service (instrumental quality control system which is an essential component of HRR) to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols. Also the Oroboros SUITbrowser will be presented. The Oroboros SUITbrowser helps you to find the best SUIT protocol for your specific research questions. Many optimized SUIT protocols are available as DL-Protocols and will be shown at the O2k-Coaching Days.


== Feedback ==
:::: [[Image:O2k-Feedback.jpg|left|60px|link=http://wiki.oroboros.at/index.php/O2k-Feedback |O2k-Feedback]] ''I had a very pleasant experience at the work shop. I thank you and all the team members for devoting their time for us and teaching each step in a systematic manner.'' - '''[[Gayathri Narayanappa]]'''




== Venue ==
== Venue ==
::::» Oroboros MitoFit Laboratory
::::» Oroboros O2k-Laboratory
::::: Schoepfstrasse 18, 6020 Innsbruck
::::: Schoepfstrasse 18, 6020 Innsbruck
::::» Information on travel and venue: '''[[IOC Innsbruck]]'''.
::::» Information on travel and venue: '''[[IOC Innsbruck]]'''.
::::» Hotel suggestion: [http://www.weisseskreuz.at/de/index.php Hotel Weisses Kreuz]
::::» Hotel recommendation: '''Basic Hotel Innsbruck***'''(https://www.basic-hotel.at/en/)
::::: Using code "''OROBOROS''" for booking online you will get the Single Room for € 85.00 and the Double Room for € 120.00 including breakfast. Rooms are available at a first come first served basis.
::::: Free cancellation until 7 days prior. Booking requires a credit card for guarantee. Payment on site. A 2 € City tax to be charged additionally.


== Registration ==
== Registration ==
  in progress
<br>
:::: Registration closed.  
<br>
== Tutors and lecturers ==


== Tutors, lecturers, and managers ==
<br/>
<br/>
<gallery mode=default perrow=5 widths="140px" heights="150px">
<gallery mode=default perrow=9 widths="140px" heights="150px">
 
File:Antunesd.jpg|'''[[Antunes D|Diana Antunes]]''', PhD., Oroboros Instruments - Scientific Quality Manager
File:CardosoLHD.JPG|'''[[Cardoso LHD|Luiza Cardoso]]''', PhD., Oroboros Instruments - PostDoc
File:CardosoLHD.JPG|'''[[Cardoso LHD|Luiza Cardoso]]''', PhD., Oroboros Instruments - PostDoc
File:Di MarcelloM.jpg|'''[[Di Marcello M|Marco Di Marcello]]''', BSc., Oroboros Instruments - Research Assistant
File:DoerrierC.JPG|'''[[Doerrier C|Carolina Doerrier]]''', PhD., Oroboros Instruments - Chief scientific officer
File:GarciaL.JPG|'''[[Garcia-Souza LF|Luiz Felipe Garcia-Souza]]''', PhD. student, Oroboros Instruments
File:Erich Gnaiger.jpg|'''[[Gnaiger E |Erich Gnaiger]]''', PhD., Oroboros Instruments - CEO


File:Di MarcelloM.jpg|'''[[Di Marcello M|Marco Di Marcello]]''', BSc., Oroboros Instruments - Scientific Assistant
File:Huete-OrtegaM.JPG| '''[[Huete-Ortega Maria| Maria Huete-Ortega]]''', PhD., Oroboros Instruments - Principal Investigator
 
File:DoerrierC.JPG|'''[[Doerrier C|Carolina Doerrier]]''', PhD. - Chief scientific officer


File:GarciaL.JPG|'''[[Garcia-Souza LF|Luiz Felipe Garcia-Souza]]''', PhD. student, Oroboros Instruments
File:KomlodiT.JPG|'''[[Komlodi T|Timea Komlodi]]''', PhD., Oroboros Instruments - Postdoc
File:Erich Gnaiger.jpg|'''[[Gnaiger E |Erich Gnaiger]]''', PhD., Oroboros Instruments, CEO
File:PassruggerM.JPG|'''[[Passrugger M|Manuela Passrugger]]''', BSc., Oroboros Instruments - Lab Manager


|-
|-
</gallery>
</gallery>


== Programme (preliminary) ==
== Programme ==
<br>
 
<div class="toccolours mw-collapsible mw-collapsed">
[[File:PDF.jpg|100px|link=https://wiki.oroboros.at/images/1/1f/MiPNet25.03_IOC144_O2k-Coaching_Days_v2.pdf |Bioblast pdf]]
<span style="font-size:105%; color:#424242">'''Monday 20th'''</span>
<div class="mw-collapsible-content">


::::{| class='wikitable'
|-
|style="width: 10em"|'''Monday 20th'''
|style="width: 60em"|'''Topic'''
|style="width: 15em"|'''Room'''
|-
|08:15
|Welcome - Get-together: Introduction of participants and their research interests
|ORO-MitoFit Lab
|-
|08:30
|OroboPOS service and O2k instrumental setup - overview with videoclips
|ORO-MitoFit Lab
|-
|09:30
|'''Hands-on''': OroboPOS service and O2k instrumental setup
|ORO-MitoFit Lab
|-
|10:10
|'''Hands-on''': DatLab 7
|ORO-MitoFit Lab
|-
|10:30
|''Coffee''
|
|-
|11:00
|Instrumental quality control 1: oxygen calibration
|ORO-MitoFit Lab
|-
|11:30
|'''Hands-on''': Instrumental quality control 1: oxygen calibration


DL-Protocol: O2k-cleaning before use
== Participants ==


DL-Protocol: O2 calibration air
{| class="wikitable"
|ORO-MitoFit Lab
|-
|12:00
|''Lunch break''
|
|-
|13:00
|'''Hands-on''':Instrumental quality control 1: oxygen calibration (continuation)
DL-Protocol: O2 calibration air
|ORO-MitoFit Lab
|-
|13:30
|Instrumental quality control 2: Instrumental O2 background – overview with videoclips
|ORO-MitoFit Lab
|-
|-
|14:00
!
|'''Hands-on''':Instrumental quality control 2: Instrumental O2 background
! Participant
DL-Protocol: Instrumental O2 background TiP2k
! Institution
|ORO-MitoFit Lab
|-
|-
|15:00
| [[File:Profile-icon-9.png|70px|Participant]]
|''Coffee''
|  [[Buoso Chiara]]
|
| '''AT Innsbruck Weiss G''' - Medical University Innsbruck, Innsbruck (AT)
|-
|-
|15:30-18:00
| [[File:BelezaJ.jpeg|100px|Participant]]
|'''Hands-on''': Instrumental quality control 1 and 2. DatLab analysis.
|  [[Beleza Jorge]]
|ORO-MitoFit Lab
| '''PT Porto Beleza J''' - University of Porto, Porto (PT)
|-
|-
|}
</div>
<br>
<br>
<div class="toccolours mw-collapsible mw-collapsed">
<span style="font-size:105%; color:#424242">'''Tuesday 21st'''</span>
<div class="mw-collapsible-content">


::::{| class='wikitable'
| [[File:Profile-icon-9.png|70px|Participant]]
|-
| [[Carter Kristen]]
|style="width: 10em"|'''Tuesday 21st'''
| '''US MS Jackson Cornelius DC''' - University of Mississippi, Jackson (US)
|style="width: 60em"|'''Topic'''
|style="width: 15em"|'''Room'''
|-
|-
|09:00
|Hands-on: Instrumental quality control 1: oxygen calibration
DL-Protocol: O2k-cleaning before use


DL-Protocol: O2 calibration air
| [[File:Chamkha Imen.jpg|70px|Chamkha Imen]]
|ORO-MitoFit Lab
|  [[Chamkha Imen]]
| '''[[SE Lund Elmer E]]''' - Lund University, Lund (SE)
|-
|-
|09:15
| [[File:Profile-icon-9.png|70px|Participant]]
|Application of the O2k Series H  
|  [[Coldewey Sina]]
|ORO-MitoFit Lab
| '''DE Jena Coldewey S''' - Jena University Hospital, Jena (DE)
|-
|-
|09:30
| [[File:Profile-icon-9.png|70px|Participant]]
|Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. SUIT reference protocol: RP1&RP2
|  [[Esper Treml Ricardo]]
|ORO-MitoFit Lab
| '''DE Jena Coldewey S''' - Jena University Hospital, Jena (DE)
|-
|-
|10:00
| [[File:Profile-icon-9.png|70px|Participant]]
|''Coffee''
|  [[Gayathri Narayanappa]]
|
| '''IN Bangalore Gayathri N''' - NIMHANS, Bangalore (IN)
|-
|-
|10:30
|'''Hands-on''': O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.


DL-Protocol: SUIT-1_O2_pce_D03.DLP
|[[File:Griffiths_Kate.JPG|60px|Kate Griffiths]]
 
|  [[Griffiths Kate]]
DL-Protocol: SUIT-2 O2 pce D07.DLP
| '''UK Glasgow Selman C''' - Glasgow University, Glasgow (UK)
 
|-
|ORO-MitoFit Lab
| [[File:Profile-icon-9.png|70px|Participant]]
|  [[Hussein Rawya]]
| '''[[SE Lund Elmer E]]''' - Lund University, Lund (SE)
|-
|-
|11:45
| [[File:Profile-icon-9.png|70px|Participant]]
|'''Hands-on''': O2k-cleaning after use
|  [[Linzi Jin]]
| '''[[CN Beijing Huawei]]''' - Huawei, Beijing (CN)


DL-Protocol: O2k-cleaning after use
|ORO-MitoFit Lab
|-
|12:00
|''Lunch break''
|
|-
|-
|13:00
| [[File:Profile-icon-9.png|70px|Participant]]
|'''Hands-on''': O2k-cleaning after use
|  [[Neu Charles]]
| '''DE Jena Coldewey S''' - Jena University Hospital, Jena (DE)


DL-Protocol: O2k-cleaning after use
|ORO-MitoFit Lab
|-
|-
|13:30
| [[File:PereiraR.jpeg|90px|Rita Pereira]]
|Introduction to DatLab analysis
| [[Pereira Rita]]
|ORO-MitoFit Lab
| '''PT Porto Beleza J''' - University of Porto, Porto (PT)
|-
|-
|15:00
| [[File:Profile-icon-9.png|70px|Participant]]
|''Coffee''
|  [[Place Nicolas]]
|
| '''[[CH Lausanne Place N]]''' - Université de Lausanne, Lausanne (CH)
|-
|-
|15:30-18:00
| [[File:Profile-icon-9.png|70px|Participant]]
|Individual DatLab analysis + summary
| Participant
|ORO-MitoFit Lab
| '''[[SE Lund Elmer E]]''' - Lund University, Lund (SE)
|-
|-
|}
|}
|}
</div>
<br>


::::{| class='wikitable'
|-
|style="width: 10em"|'''Wednesday 12th'''
|style="width: 60em"|'''Topic'''
|style="width: 15em"|'''Room'''
|-
|09:00
|'''MitoFit proficiency test:'''


'''Hands-on''': Instrumental quality control 1: oxygen calibration


DL-Protocol: O2k-cleaning before use
== Impressions from the IOC144 ==


DL-Protocol: O2 calibration air
<gallery mode=default perrow=4 widths="350px" heights="350px">


|ORO-MitoFit Lab
File:IOC144 5354.JPG
|-
File:IOC144 5349.JPG
|10:00
File:IOC144 5331.JPG
|''Coffee''
File:IOC144 5327.JPG
|
|-
|10:30
|'''MitoFit proficiency test:'''


'''Hands-on''': O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.


DL-Protocol: SUIT-1_O2_pce_D03.DLP
</gallery>


DL-Protocol: SUIT-2 O2 pce D07.DLP
|ORO-MitoFit Lab
|-
|11:45
|'''Hands-on''': O2k-cleaning after use
DL-Protocol: O2k-cleaning after use
|ORO-MitoFit Lab
|-
|12:00
|Lunch break
|
|-
|13:00
|'''Hands-on''': O2k-cleaning after use
DL-Protocol: O2k-cleaning after use
|ORO-MitoFit Lab
|-
|13:15
|DatLab analysis
|ORO-MitoFit Lab
|-
|14:00
|DatLab analysis summary
|ORO-MitoFit Lab
|-
|15:00
|''Coffee''
|
|-
|15:30
|The Bioblast wiki, O2k-Network and MITOEAGLE
|MiP''Art''
|-
|16:15
|Feedback & conclusions
|MiP''Art''
|-
|17:00
|Farewell activity
|MiP''Art''
|-
|}
== Participants ==


== Support ==
{{NextGen-O2k H2020-support}}
:::: Supported by project NextGen-O2k which has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 859770.
<br/>
<br/>
<br/>


== Contact ==
== Contact ==
Line 280: Line 168:
:::: E-Mail: [mailto:[email protected]| [email protected]]
:::: E-Mail: [mailto:[email protected]| [email protected]]
:::: '''Mitochondria and Cell Research'''
:::: '''Mitochondria and Cell Research'''


== IOC recommended reading ==
== IOC recommended reading ==
::::» '''[[IOC recommended reading]]'''
::::» '''[[IOC recommended reading]]'''
::::» '''[[MitoPedia: Respiratory states]]'''
::::» '''[[MitoPedia: Respiratory states]]'''
:::: [[Image:P.jpg|link=OXPHOS-capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link=ET-capacity|ETS]] [[Image:L.jpg|link=LEAK respiration|LEAK]] [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]
:::: [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link=ET capacity|ETS]] [[Image:L.jpg|link=LEAK respiration|LEAK]] [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]
 


== Project support==
:::: Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
:::: [[File:Sponsors.png| 750 px]]


[[Image:MitoGlobal.jpg|right|80px|link=MitoGlobal|MitoGlobal]]  
[[Image:MitoGlobal.jpg|right|80px|link=MitoGlobal|MitoGlobal]]  
  O2k-Workshops and MitoFit Coaching Days are listed as [[MitoGlobal Events]].
  O2k-Workshops and O2k-Coaching Days are listed as [[MitoGlobal Events]].


{{Labeling
{{Labeling
|additional=ORO, IOC, MitoFit, MitoGlobal, Next, 2020,
|additional=ORO, IOC, MitoGlobal, 2020, NextGen-O2k
}}
}}


[[Category:O2k-Workshops]]
[[Category:O2k-Workshops]]

Latest revision as of 10:50, 22 November 2021

                

MiPNet25.03 IOC144 Innsbruck AT


Publications in the MiPMap
OROBOROS-solo.png

Innsbruck AT, 2020 Jan 20-22. O2k-Coaching Days - Basic IOC144.

» Bioblast pdf

Oroboros (2020-01-20) Mitochondr Physiol Network

Abstract:

O2k-Workshops.png

O2k-Coaching Days - Basic. Innsbruck, Austria; 2020 Jan 20-22.


• O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit


O2k-Workshops

General information

The course is over.
O2k-Coaching Days - Basic is a training course which provides a fundamental introduction to high-resolution respirometry (HRR) with the Oroboros O2k. We will give you an overview of the O2k-FluoRespirometer, including real-time analysis with DatLab 7.4 and applications of the Titration-Injection microPump TIP2k. Hands-on sessions range from OroboPOS technical service (instrumental quality control system which is an essential component of HRR) to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols. Also the Oroboros SUITbrowser will be presented. The Oroboros SUITbrowser helps you to find the best SUIT protocol for your specific research questions. Many optimized SUIT protocols are available as DL-Protocols and will be shown at the O2k-Coaching Days.

Feedback

O2k-Feedback
I had a very pleasant experience at the work shop. I thank you and all the team members for devoting their time for us and teaching each step in a systematic manner. - Gayathri Narayanappa


Venue

» Oroboros O2k-Laboratory
Schoepfstrasse 18, 6020 Innsbruck
» Information on travel and venue: IOC Innsbruck.
» Hotel recommendation: Basic Hotel Innsbruck***(https://www.basic-hotel.at/en/)
Using code "OROBOROS" for booking online you will get the Single Room for € 85.00 and the Double Room for € 120.00 including breakfast. Rooms are available at a first come first served basis.
Free cancellation until 7 days prior. Booking requires a credit card for guarantee. Payment on site. A 2 € City tax to be charged additionally.

Registration


Registration closed.


Tutors and lecturers


Programme

Bioblast pdf


Participants

Participant Institution
Participant Buoso Chiara AT Innsbruck Weiss G - Medical University Innsbruck, Innsbruck (AT)
Participant Beleza Jorge PT Porto Beleza J - University of Porto, Porto (PT)
Participant Carter Kristen US MS Jackson Cornelius DC - University of Mississippi, Jackson (US)
Chamkha Imen Chamkha Imen SE Lund Elmer E - Lund University, Lund (SE)
Participant Coldewey Sina DE Jena Coldewey S - Jena University Hospital, Jena (DE)
Participant Esper Treml Ricardo DE Jena Coldewey S - Jena University Hospital, Jena (DE)
Participant Gayathri Narayanappa IN Bangalore Gayathri N - NIMHANS, Bangalore (IN)
Kate Griffiths Griffiths Kate UK Glasgow Selman C - Glasgow University, Glasgow (UK)
Participant Hussein Rawya SE Lund Elmer E - Lund University, Lund (SE)
Participant Linzi Jin CN Beijing Huawei - Huawei, Beijing (CN)
Participant Neu Charles DE Jena Coldewey S - Jena University Hospital, Jena (DE)
Rita Pereira Pereira Rita PT Porto Beleza J - University of Porto, Porto (PT)
Participant Place Nicolas CH Lausanne Place N - Université de Lausanne, Lausanne (CH)
Participant Participant SE Lund Elmer E - Lund University, Lund (SE)


Impressions from the IOC144

  • IOC144 5354.JPG
  • IOC144 5349.JPG
  • IOC144 5331.JPG
  • IOC144 5327.JPG


Support

Template NextGen-O2k.jpg
Supported by project NextGen-O2k which has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 859770.




Contact

Oroboros Instruments GmbH
High-Resolution Respirometry
Schoepfstrasse 18
A-6020 Innsbruck, Austria
Tel: +43 512 566796
Fax: +43 512 566796 20
E-Mail: [email protected]
Mitochondria and Cell Research

IOC recommended reading

» IOC recommended reading
» MitoPedia: Respiratory states
OXPHOS ROUTINE ETS LEAK ROX


MitoGlobal
O2k-Workshops and O2k-Coaching Days are listed as MitoGlobal Events.


Labels:






ORO, IOC, MitoGlobal, 2020, NextGen-O2k 

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