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Difference between revisions of "MiPNet25.03 IOC144 Innsbruck AT"

From Bioblast
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{{Publication
{{Publication
|title=[[Image:OROBOROS-solo.png|right|60px|link=OROBOROS INSTRUMENTS]]
|title=[[Image:OROBOROS-solo.png|right|60px|link=OROBOROS INSTRUMENTS]]
'''Innsbruck AT''', 2020 Jan 20-22. MitoFit Coaching Days - Basic '''IOC144'''.
'''Innsbruck AT''', 2020 Jan 20-22. O2k-Coaching Days - Basic '''IOC144'''.
|authors=Oroboros
|authors=Oroboros
|year=2020-01-20
|year=2020-01-20
|journal=Mitochondr Physiol Network
|journal=Mitochondr Physiol Network
|abstract=[[Image:O2k-Workshops.png|left|130px|link=O2k-Workshops]]
|abstract=[[Image:O2k-Workshops.png|left|130px|link=O2k-Workshops]]
'''MitoFit Coaching Days - Basic'''. Innsbruck, Austria; 2020 Jan 20-22.
'''O2k-Coaching Days - Basic'''. Innsbruck, Austria; 2020 Jan 20-22.
|mipnetlab=AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit
|mipnetlab=AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit
}}
}}
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:::: ''' The course is fully booked. In case of cancellations further registrations can be accepted. In order to be added to the waiting list, send an email to [mailto:[email protected] [email protected]]'''
:::: ''' The course is fully booked. In case of cancellations further registrations can be accepted. In order to be added to the waiting list, send an email to [mailto:[email protected] [email protected]]'''


:::: '''MitoFit Coaching Days - Basic''' is a training course which provides a fundamental introduction to high-resolution respirometry (HRR) with the Oroboros O2k. We will give you an overview of the O2k-FluoRespirometer, including real-time analysis with DatLab 7.4 and applications of the Titration-Injection microPump TIP2k. Hands-on sessions range from OroboPOS technical service (instrumental quality control system which is an essential component of HRR) to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols. Also the new Oroboros SUITbrowser will be presented. The SUITbrowser helps you to find the best SUIT protocol for your specific research questions. Many optimized SUIT protocols are available as DL-Protocols and will be shown at the Coaching Days.
:::: '''O2k-Coaching Days - Basic''' is a training course which provides a fundamental introduction to high-resolution respirometry (HRR) with the Oroboros O2k. We will give you an overview of the O2k-FluoRespirometer, including real-time analysis with DatLab 7.4 and applications of the Titration-Injection microPump TIP2k. Hands-on sessions range from OroboPOS technical service (instrumental quality control system which is an essential component of HRR) to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols. Also the new Oroboros SUITbrowser will be presented. The SUITbrowser helps you to find the best SUIT protocol for your specific research questions. Many optimized SUIT protocols are available as DL-Protocols and will be shown at the O2k-Coaching Days.




== Venue ==
== Venue ==
::::» Oroboros MitoFit Laboratory
::::» Oroboros O2k-Laboratory
::::: Schoepfstrasse 18, 6020 Innsbruck
::::: Schoepfstrasse 18, 6020 Innsbruck
::::» Information on travel and venue: '''[[IOC Innsbruck]]'''.
::::» Information on travel and venue: '''[[IOC Innsbruck]]'''.
::::» Hotel recommendation: '''Basic Hotel Innsbruck***'''(https://www.basic-hotel.at/en/)
::::» Hotel recommendation: '''Basic Hotel Innsbruck***'''(https://www.basic-hotel.at/en/)
::::: Using code "''OROBOROS''" for booking online you will get the Single Room for € 85.00 and the Double Room for € 120.00 including breakfast. Rooms are available at a first come first served basis.
::::: Using code "''OROBOROS''" for booking online you will get the Single Room for € 85.00 and the Double Room for € 120.00 including breakfast. Rooms are available at a first come first served basis.
::::: Free cancellation until 7 days prior. Booking requires a credit card for guarantee. Payment on site. A 2€ City tax to be charged additionally.
::::: Free cancellation until 7 days prior. Booking requires a credit card for guarantee. Payment on site. A 2 € City tax to be charged additionally.
 
== Registration ==
== Registration ==
<br>
<br>
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|08:15
|08:15
|Welcome - Get-together: Introduction of participants and their research interests
|Welcome - Get-together: Introduction of participants and their research interests
|Oroboros MitoFit Laboratory
|Oroboros O2k-Laboratory
|-
|-
|08:30
|08:30
|OroboPOS service and O2k instrumental setup - overview with videoclips  
|OroboPOS service and O2k instrumental setup - overview with videoclips  
|Oroboros MitoFit Laboratory
|Oroboros O2k-Laboratory
|-
|-
|09:15
|09:15
|'''Hands-on''': OroboPOS service and O2k instrumental setup  
|'''Hands-on''': OroboPOS service and O2k instrumental setup  
|Oroboros MitoFit Laboratory
|Oroboros O2k-Laboratory
|-
|-
|09:55
|09:55
|DatLab 7.4 overview  
|DatLab 7.4 overview  
|Oroboros MitoFit Laboratory
|Oroboros O2k-Laboratory
|-
|-
|10:30
|10:30
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|11:00
|11:00
|Instrumental quality control 1: oxygen calibration  
|Instrumental quality control 1: oxygen calibration  
|Oroboros MitoFit Laboratory
|Oroboros O2k-Laboratory
|-
|-
|11:30
|11:30
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DL-Protocol: O2 calibration air
DL-Protocol: O2 calibration air
|Oroboros MitoFit Laboratory
|Oroboros O2k-Laboratory
|-
|-
|12:00
|12:00
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|'''Hands-on''': Instrumental quality control 1: oxygen calibration (continuation)
|'''Hands-on''': Instrumental quality control 1: oxygen calibration (continuation)
DL-Protocol: O2 calibration air  
DL-Protocol: O2 calibration air  
|Oroboros MitoFit Laboratory
|Oroboros O2k-Laboratory
|-
|-
|13:30
|13:30
|Instrumental quality control 2: Instrumental O<sub>2</sub> background – overview with videoclips
|Instrumental quality control 2: Instrumental O<sub>2</sub> background – overview with videoclips
|Oroboros MitoFit Laboratory
|Oroboros O2k-Laboratory
|-
|-
|14:00
|14:00
|'''Hands-on''':Instrumental quality control 2: Instrumental O<sub>2</sub> background  
|'''Hands-on''':Instrumental quality control 2: Instrumental O<sub>2</sub> background  
DL-Protocol: Instrumental O2 background TiP2k
DL-Protocol: Instrumental O2 background TiP2k
|Oroboros MitoFit Laboratory
|Oroboros O2k-Laboratory
|-
|-
|15:00
|15:00
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|15:30-17:30
|15:30-17:30
|'''Hands-on''': Instrumental quality control 1 and 2. DatLab analysis.
|'''Hands-on''': Instrumental quality control 1 and 2. DatLab analysis.
|Oroboros MitoFit Laboratory
|Oroboros O2k-Laboratory
|-
|-
|}
|}
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DL-Protocol: O2 calibration air
DL-Protocol: O2 calibration air
|Oroboros MitoFit Laboratory
|Oroboros O2k-Laboratory
|-
|-
|09:15
|09:15
|Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. SUIT reference protocol: RP1&RP2  
|Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. SUIT reference protocol: RP1&RP2  
|Oroboros MitoFit Laboratory
|Oroboros O2k Laboratory
|-
|-
|09:45
|09:45
|SUITbrowser: how to find the best SUIT protocol for your research questions.  
|SUITbrowser: how to find the best SUIT protocol for your research questions.  
|Oroboros MitoFit Laboratory
|Oroboros O2k Laboratory
|-
|-
|10:00
|10:00
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DL-Protocol: SUIT-002 O2 ce-pce D007.DLP   
DL-Protocol: SUIT-002 O2 ce-pce D007.DLP   


|Oroboros MitoFit Laboratory
|Oroboros O2k Laboratory
|-
|-
|11:45
|11:45
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DL-Protocol: O2k-cleaning AfterUse
DL-Protocol: O2k-cleaning AfterUse
|Oroboros MitoFit Laboratory
|Oroboros O2k-Laboratory
|-
|-
|12:00
|12:00
Line 179: Line 180:


DL-Protocol: O2k-cleaning AfterUse
DL-Protocol: O2k-cleaning AfterUse
|Oroboros MitoFit Laboratory
|Oroboros O2k-Laboratory
|-
|-
|13:30
|13:30
|DatLab 7.4 analysis: Introduction and new features
|DatLab 7.4 analysis: Introduction and new features
|Oroboros MitoFit Laboratory
|Oroboros O2k-Laboratory
|-
|-
|15:00
|15:00
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|15:30-17:30
|15:30-17:30
|Individual DatLab analysis + summary
|Individual DatLab analysis + summary
|Oroboros MitoFit Laboratory
|Oroboros O2k-Laboratory
|-
|-
|}
|}
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DL-Protocol: O2 calibration air
DL-Protocol: O2 calibration air


|Oroboros MitoFit Laboratory
|Oroboros O2k-Laboratory
|-
|-
|10:00
|10:00
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DL-Protocol: SUIT-002 O2 ce-pce D007.DLP  
DL-Protocol: SUIT-002 O2 ce-pce D007.DLP  


|Oroboros MitoFit Laboratory
|Oroboros O2k-Laboratory
|-
|-
|11:45
|11:45
Line 238: Line 239:


DL-Protocol: O2k-cleaning AfterUse
DL-Protocol: O2k-cleaning AfterUse
|Oroboros MitoFit Laboratory
|Oroboros O2k-Laboratory
|-
|-
|12:00
|12:00
Line 248: Line 249:


DL-Protocol: O2k-cleaning AfterUse
DL-Protocol: O2k-cleaning AfterUse
|Oroboros MitoFit Laboratory
|Oroboros O2k-Laboratory
|-
|-
|13:15
|13:15
|DatLab analysis
|DatLab analysis
|Oroboros MitoFit Laboratory
|Oroboros O2k-Laboratory
|-
|-
|14:00
|14:00
|O2k-Applications - overview
|O2k-Applications - overview
|Oroboros MitoFit Laboratory
|Oroboros O2k-Laboratory
|-
|-
|14:30
|14:30
|Proficiency test: DatLab analysis summary
|Proficiency test: DatLab analysis summary
|Oroboros MitoFit Laboratory
|Oroboros O2k-Laboratory
|-
|-
|15:00
|15:00
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[[Image:MitoGlobal.jpg|right|80px|link=MitoGlobal|MitoGlobal]]  
[[Image:MitoGlobal.jpg|right|80px|link=MitoGlobal|MitoGlobal]]  
  O2k-Workshops and MitoFit Coaching Days are listed as [[MitoGlobal Events]].
  O2k-Workshops and O2k-Coaching Days are listed as [[MitoGlobal Events]].


{{Labeling
{{Labeling

Revision as of 03:27, 19 November 2019

                

MiPNet25.03 IOC144 Innsbruck AT


Publications in the MiPMap
OROBOROS-solo.png

Innsbruck AT, 2020 Jan 20-22. O2k-Coaching Days - Basic IOC144.


Oroboros (2020-01-20) Mitochondr Physiol Network

Abstract:

O2k-Workshops.png

O2k-Coaching Days - Basic. Innsbruck, Austria; 2020 Jan 20-22.


• O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit

Template NextGen-O2k.jpg


General information

The course is fully booked. In case of cancellations further registrations can be accepted. In order to be added to the waiting list, send an email to [email protected]
O2k-Coaching Days - Basic is a training course which provides a fundamental introduction to high-resolution respirometry (HRR) with the Oroboros O2k. We will give you an overview of the O2k-FluoRespirometer, including real-time analysis with DatLab 7.4 and applications of the Titration-Injection microPump TIP2k. Hands-on sessions range from OroboPOS technical service (instrumental quality control system which is an essential component of HRR) to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols. Also the new Oroboros SUITbrowser will be presented. The SUITbrowser helps you to find the best SUIT protocol for your specific research questions. Many optimized SUIT protocols are available as DL-Protocols and will be shown at the O2k-Coaching Days.


Venue

» Oroboros O2k-Laboratory
Schoepfstrasse 18, 6020 Innsbruck
» Information on travel and venue: IOC Innsbruck.
» Hotel recommendation: Basic Hotel Innsbruck***(https://www.basic-hotel.at/en/)
Using code "OROBOROS" for booking online you will get the Single Room for € 85.00 and the Double Room for € 120.00 including breakfast. Rooms are available at a first come first served basis.
Free cancellation until 7 days prior. Booking requires a credit card for guarantee. Payment on site. A 2 € City tax to be charged additionally.

Registration


Registration closed.


Tutors and lecturers


Preliminary programme


Monday 20th

Topic Room
08:15 Welcome - Get-together: Introduction of participants and their research interests Oroboros O2k-Laboratory
08:30 OroboPOS service and O2k instrumental setup - overview with videoclips Oroboros O2k-Laboratory
09:15 Hands-on: OroboPOS service and O2k instrumental setup Oroboros O2k-Laboratory
09:55 DatLab 7.4 overview Oroboros O2k-Laboratory
10:30 Coffee
11:00 Instrumental quality control 1: oxygen calibration Oroboros O2k-Laboratory
11:30 Hands-on: Instrumental quality control 1: oxygen calibration

DL-Protocol: O2k-cleaning BeforeUse

DL-Protocol: O2 calibration air

Oroboros O2k-Laboratory
12:00 Lunch break
13:00 Hands-on: Instrumental quality control 1: oxygen calibration (continuation)

DL-Protocol: O2 calibration air

Oroboros O2k-Laboratory
13:30 Instrumental quality control 2: Instrumental O2 background – overview with videoclips Oroboros O2k-Laboratory
14:00 Hands-on:Instrumental quality control 2: Instrumental O2 background

DL-Protocol: Instrumental O2 background TiP2k

Oroboros O2k-Laboratory
15:00 Coffee
15:30-17:30 Hands-on: Instrumental quality control 1 and 2. DatLab analysis. Oroboros O2k-Laboratory


Tuesday 21st

Topic Room
08:30 Hands-on: Instrumental quality control 1: oxygen calibration

DL-Protocol: O2k-cleaning BeforeUse

DL-Protocol: O2 calibration air

Oroboros O2k-Laboratory
09:15 Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. SUIT reference protocol: RP1&RP2 Oroboros O2k Laboratory
09:45 SUITbrowser: how to find the best SUIT protocol for your research questions. Oroboros O2k Laboratory
10:00 Coffee
10:30 Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with the reference protocol RP1 (SUIT-001) and RP2 (SUIT-002).

DL-Protocol: SUIT-001 O2 ce-pce D003.DLP

DL-Protocol: SUIT-002 O2 ce-pce D007.DLP

Oroboros O2k Laboratory
11:45 Hands-on: O2k-cleaning after use

DL-Protocol: O2k-cleaning AfterUse

Oroboros O2k-Laboratory
12:00 Lunch break
13:00 Hands-on: O2k-cleaning after use (continuation)

DL-Protocol: O2k-cleaning AfterUse

Oroboros O2k-Laboratory
13:30 DatLab 7.4 analysis: Introduction and new features Oroboros O2k-Laboratory
15:00 Coffee
15:30-17:30 Individual DatLab analysis + summary Oroboros O2k-Laboratory


Wednesday 22nd

Topic Room
08:30 MitoFit proficiency test

Hands-on: Instrumental quality control 1: oxygen calibration

DL-Protocol: O2k-cleaning BeforeUse

DL-Protocol: O2 calibration air

Oroboros O2k-Laboratory
10:00 Coffee
10:30 MitoFit proficiency test

Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with the reference protocol RP1 (SUIT-001) and RP2 (SUIT-002).

DL-Protocol: SUIT-001 O2 ce-pce D003.DLP

DL-Protocol: SUIT-002 O2 ce-pce D007.DLP

Oroboros O2k-Laboratory
11:45 Hands-on: O2k-cleaning after use

DL-Protocol: O2k-cleaning AfterUse

Oroboros O2k-Laboratory
12:00 Lunch break
13:00 Hands-on: O2k-cleaning after use (continuation)

DL-Protocol: O2k-cleaning AfterUse

Oroboros O2k-Laboratory
13:15 DatLab analysis Oroboros O2k-Laboratory
14:00 O2k-Applications - overview Oroboros O2k-Laboratory
14:30 Proficiency test: DatLab analysis summary Oroboros O2k-Laboratory
15:00 Coffee
15:30 The Bioblast wiki, MitoFit Preprint Archives, O2k-Network and MitoEAGLE MiPArt
16:30 Feedback & conclusions MiPArt
17:00 Farewell activity MiPArt

Participants

Participant Institution
Participant Buoso Chiara AT Innsbruck Weiss G - Medical University Innsbruck, Innsbruck (AT)
Participant Carter Kristen US MS Jackson Cornelius DC - University of Mississippi, Jackson (US)
Chamkha Imen Chamkha Imen SE Lund Elmer E - Lund University, Lund (SE)
Participant Coldewey Sina DE Jena Coldewey S - Jena University Hospital, Jena (DE)
Participant Esper Treml Ricardo DE Jena Coldewey S - Jena University Hospital, Jena (DE)
Participant Gayathri Narayanappa IN Bangalore Gayathri N - NIMHANS, Bangalore (IN)
Kate Griffiths Griffiths Kate UK Glasgow Selman C - Glasgow University, Glasgow (UK)
Participant Hussein Rawya SE Lund Elmer E - Lund University, Lund (SE)
Participant Neu Charles DE Jena Coldewey S - Jena University Hospital, Jena (DE)
Participant Place Nicolas CH Lausanne Place N - Université de Lausanne, Lausanne (CH)
Participant Participant SE Lund Elmer E - Lund University, Lund (SE)

Contact

Oroboros Instruments GmbH
High-Resolution Respirometry
Schoepfstrasse 18
A-6020 Innsbruck, Austria
Tel: +43 512 566796
Fax: +43 512 566796 20
E-Mail: [email protected]
Mitochondria and Cell Research


IOC recommended reading

» IOC recommended reading
» MitoPedia: Respiratory states
OXPHOS ROUTINE ETS LEAK ROX


MitoGlobal
O2k-Workshops and O2k-Coaching Days are listed as MitoGlobal Events.


Labels:






ORO, IOC, MitoGlobal, Next, 2020 

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