MiPNet25.03 IOC144 Innsbruck AT

                

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MiPNet25.03 IOC144 Innsbruck AT

Innsbruck AT, 2020 Jan 20-22. MitoFit Coaching Days - Basic IOC143.

Oroboros (2020-01-20) Mitochondr Physiol Network

Abstract:

MitoFit Coaching Days - Basic. Innsbruck, Austria; 2020 Jan 20-22.

• O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit

General information

MitoFit Coaching Days - Basic is a training course which provides a fundamental introduction to high-resolution respirometry (HRR) with the Oroboros O2k. We will give you an overview of the O2k-FluoRespirometer, including real-time analysis with DatLab 7.4 and applications of the Titration-Injection microPump TIP2k. Hands-on sessions range from OroboPOS technical service (instrumental quality control system which is an essential component of HRR) to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols. Also the new Oroboros SUITbrowser will be presented. The SUITbrowser helps you to find the best SUIT protocol for your specific research questions. Many optimized SUIT protocols are available as DL-Protocols adn will be shown at the Coaching Days.

Venue

» Oroboros MitoFit Laboratory

Schoepfstrasse 18, 6020 Innsbruck

» Information on travel and venue: IOC Innsbruck.

» Hotel recommendation: Basic Hotel Innsbruck***(https://www.basic-hotel.at/en/)

Using code OROBOROS for booking online you will get the Single Room for € 85.00 and the Double Room for € 120.00 including breakfast. Rooms are available at a first come first served basis.

Free cancellation until 7 days prior. Booking requires a credit card for guarantee. Payment on site. A 2€ City tax to be charged additionally.

Registration

Download registration form, fill in the pdf and the print to sign: download pdf

Tutors and lecturers

• 

  Luiza Cardoso, PhD., Oroboros Instruments - PostDoc

• 

  Marco Di Marcello, BSc., Oroboros Instruments - Scientific Assistant

• 

  Carolina Doerrier, PhD. - Chief scientific officer

• 

  Luiz Felipe Garcia-Souza, PhD. student, Oroboros Instruments

• 

  Erich Gnaiger, PhD., Oroboros Instruments, CEO

Preliminary programme

Monday 20th

	Topic	Room
08:15	Welcome - Get-together: Introduction of participants and their research interests	ORO-MitoFit Lab
08:30	OroboPOS service and O2k instrumental setup - overview with videoclips	ORO-MitoFit Lab
09:30	Hands-on: OroboPOS service and O2k instrumental setup	ORO-MitoFit Lab
10:10	Hands-on: DatLab 7	ORO-MitoFit Lab
10:30	Coffee
11:00	Instrumental quality control 1: oxygen calibration	ORO-MitoFit Lab
11:30	Hands-on: Instrumental quality control 1: oxygen calibration DL-Protocol: O2k-cleaning before use DL-Protocol: O2 calibration air	ORO-MitoFit Lab
12:00	Lunch break
13:00	Hands-on:Instrumental quality control 1: oxygen calibration (continuation) DL-Protocol: O2 calibration air	ORO-MitoFit Lab
13:30	Instrumental quality control 2: Instrumental O2 background – overview with videoclips	ORO-MitoFit Lab
14:00	Hands-on:Instrumental quality control 2: Instrumental O2 background DL-Protocol: Instrumental O2 background TiP2k	ORO-MitoFit Lab
15:00	Coffee
15:30-18:00	Hands-on: Instrumental quality control 1 and 2. DatLab analysis.	ORO-MitoFit Lab

Tuesday 21st

	Topic	Room
09:00	Hands-on: Instrumental quality control 1: oxygen calibration DL-Protocol: O2k-cleaning before use DL-Protocol: O2 calibration air	ORO-MitoFit Lab
09:15	Application of the O2k Series H	ORO-MitoFit Lab
09:30	Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. SUIT reference protocol: RP1&RP2	ORO-MitoFit Lab
10:00	Coffee
10:30	Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2. DL-Protocol: SUIT-1_O2_pce_D03.DLP DL-Protocol: SUIT-2 O2 pce D07.DLP	ORO-MitoFit Lab
11:45	Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning after use	ORO-MitoFit Lab
12:00	Lunch break
13:00	Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning after use	ORO-MitoFit Lab
13:30	Introduction to DatLab analysis	ORO-MitoFit Lab
15:00	Coffee
15:30-18:00	Individual DatLab analysis + summary	ORO-MitoFit Lab

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Wednesday 22nd

	Topic	Room
09:00	MitoFit proficiency test: Hands-on: Instrumental quality control 1: oxygen calibration DL-Protocol: O2k-cleaning before use DL-Protocol: O2 calibration air	ORO-MitoFit Lab
10:00	Coffee
10:30	MitoFit proficiency test: Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2. DL-Protocol: SUIT-1_O2_pce_D03.DLP DL-Protocol: SUIT-2 O2 pce D07.DLP	ORO-MitoFit Lab
11:45	Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning after use	ORO-MitoFit Lab
12:00	Lunch break
13:00	Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning after use	ORO-MitoFit Lab
13:15	DatLab analysis	ORO-MitoFit Lab
14:00	DatLab analysis summary	ORO-MitoFit Lab
15:00	Coffee
15:30	The Bioblast wiki, O2k-Network and MITOEAGLE	MiPArt
16:15	Feedback & conclusions	MiPArt
17:00	Farewell activity	MiPArt

Participants

	Participant	Institution
	Griffiths Kate	UK Glasgow Selman C - Glasgow University, Glasgow (UK)

Contact

Oroboros Instruments GmbH

High-Resolution Respirometry

Schoepfstrasse 18

A-6020 Innsbruck, Austria

Tel: +43 512 566796

Fax: +43 512 566796 20

E-Mail: [email protected]

Mitochondria and Cell Research

IOC recommended reading

» IOC recommended reading

» MitoPedia: Respiratory states

O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.

Labels:

ORO, IOC, MitoGlobal, Next, 2020