Difference between revisions of "MiPNet20.13 Safranin mt-membranepotential"
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:» The OROBOROS MitoLab uses [[Safranin]] O from Sigma (#S2255, 25 g). | :» The [[OROBOROS Mitochondrial Research Laboratory |OROBOROS MitoLab]] uses [[Safranin]] O from Sigma (#S2255, 25 g). | ||
:» More details: [[Talk:MiPNet20.13 Safranin mt-membranepotential |»Discussion«]]. | :» More details: [[Talk:MiPNet20.13 Safranin mt-membranepotential |»Discussion«]]. | ||
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=== Substances with an effect on the fluorescence signal of safranin === | === Substances with an effect on the fluorescence signal of safranin === | ||
* ADP (D) | :* ADP (D) | ||
* Cytochrome ''c'' (c) | :* Cytochrome ''c'' (c) | ||
* Succinate (S) | :* Succinate (S) | ||
* Rotenone (Rot) | :* Rotenone (Rot) | ||
* Ascorbate (As) | :* Ascorbate (As) | ||
* TMPD (Tm) | :* TMPD (Tm) | ||
=== Substances without an effect on the fluorescence signal of safranin === | === Substances without an effect on the fluorescence signal of safranin === | ||
: * Pyruvate (P) | |||
* Pyruvate (P) | :* Malate (M) | ||
* Malate (M) | :* Glutamate (G) | ||
* Glutamate (G) | :* Digitonin (Dig) | ||
* Digitonin (Dig) | :* Oligomycin (Omy) | ||
* Oligomycin (Omy) | :* FCCP (U) | ||
* FCCP (U) | :* Malonic acid (Mna) | ||
* Malonic acid (Mna) | :* Antimycin A (Ama) | ||
* Antimycin A (Ama) | :* DMSO | ||
* DMSO | :* Ethanol | ||
* Ethanol | :* H<sub>2</sub>O<sub>2</sub> | ||
* H<sub>2</sub>O<sub>2</sub> | :* H<sub>2</sub>O | ||
* H<sub>2</sub>O | |||
Revision as of 14:21, 12 June 2015
 |
» Versions
OROBOROS (2015-06-09) Mitochondr Physiol Network
Abstract: Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2015) O2k-Fluorometry: HRR and simultaneous determination of mt-membrane potential with safranin. Mitochondr Physiol Network 20.13(01):1-5.
Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. »Bioblast link«
• O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels: MiParea: Respiration, Instruments;methods
Organism: Mouse
Tissue;cell: Nervous system
Preparation: Homogenate
Coupling state: LEAK, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, O2k-Fluorometer, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-Demo, O2k-MultiSensor
- » The OROBOROS MitoLab uses Safranin O from Sigma (#S2255, 25 g).
- » More details: »Discussion«.
Safranin chemical background
- Several substances typically used in SUIT protocols may influence the fluorescence signal of safranin when injected into the O2k-Chamber (for instance coloured substances such as cytochrome c). These chemicals should be tested for their effect in a background run without biological sample, and the necessary corrections be applied.
Substances with an effect on the fluorescence signal of safranin
- ADP (D)
- Cytochrome c (c)
- Succinate (S)
- Rotenone (Rot)
- Ascorbate (As)
- TMPD (Tm)
Substances without an effect on the fluorescence signal of safranin
- * Pyruvate (P)
- Malate (M)
- Glutamate (G)
- Digitonin (Dig)
- Oligomycin (Omy)
- FCCP (U)
- Malonic acid (Mna)
- Antimycin A (Ama)
- DMSO
- Ethanol
- H2O2
- H2O
