Difference between revisions of "MiPNet20.13 Safranin mt-membranepotential"

Latest revision as of 11:26, 27 October 2023

MiPNet20.13 Safranin mt-membranepotential

O2k-FluoRespirometry: HRR and simultaneous determination of mt-membrane potential with safranin or TMRM.

» »Versions

Oroboros (2019-06-24) Mitochondr Physiol Network

Abstract: Krumschnabel G, Fasching M, Gnaiger E (2019) O2k-FluoRespirometry: HRR and simultaneous determination of mt-membrane potential with safranin or TMRM. Mitochondr Physiol Network 20.13(03):1-5.

Open the pdf document above.

» Current O2k-series: NextGen-O2k Series XB and O2k Series J

» Current software versions DatLab 8.0: MitoPedia: DatLab

Further details: » MitoPedia: O2k-Open Support

• O2k-Network Lab: AT_Innsbruck_Oroboros

Labels: MiParea: Respiration, Instruments;methods

Organism: Mouse Tissue;cell: Nervous system Preparation: Homogenate

Coupling state: LEAK, ET Pathway: S, ROX HRR: Oxygraph-2k, O2k-Fluorometer, O2k-Protocol

O2k-Demo, O2k-MultiSensor, MitoFitPublication, SUIT-020, SUIT-020 Fluo mt D033, SUIT-006 Fluo mt D034, SUIT-021 Fluo mt D036, SUIT-021, mt-Membrane

@@ Line 1: / Line 1: @@
+{{OROBOROS header page name}}
 {{Publication
-|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] O2k-Fluorometry: HRR and simultaneous determination of mt-membrane potential with safranin. [[Media:MiPNet20.13_Safranin_mt-membranepotential.pdf |»Bioblast pdf«]]
+|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] O2k-FluoRespirometry: HRR and simultaneous determination of mt-membrane potential with safranin or TMRM.
-|info=[http://www.bioblast.at/index.php/File:MiPNet20.13_Safranin_mt-membranepotential.pdf Versions]
+|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/5/52/MiPNet20.13_Safranin_mt-membranepotential.pdf |Bioblast pdf]] »[http://www.bioblast.at/index.php/File:MiPNet20.13_Safranin_mt-membranepotential.pdf Versions]
-|authors=OROBOROS
+|authors=Oroboros
-|year=2015-06-09
+|year=2019-06-24
 |journal=Mitochondr Physiol Network
-|abstract=Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2015) O2k-Fluorometry: HRR and simultaneous determination of mt-membrane potential with safranin. Mitochondr Physiol Network 20.13(01):1-5.
+|abstract=Krumschnabel G, Fasching M, Gnaiger E (2019) O2k-FluoRespirometry: HRR and simultaneous determination of mt-membrane potential with [[safranin]] or [[TMRM]]. Mitochondr Physiol Network 20.13(03):1-5.
-|mipnetlab=AT_Innsbruck_OROBOROS
+{{MiPNet pdf page linking to MitoPedia}}
+|mipnetlab=AT_Innsbruck_Oroboros
 }}
 {{Labeling
@@ Line 13: / Line 15: @@
 |tissues=Nervous system
 |preparations=Homogenate
-|couplingstates=LEAK, ETS
+|couplingstates=LEAK, ET
-|substratestates=CII, ROX
+|pathways=S, ROX
-|instruments=Oxygraph-2k, O2k-Fluorometer, Protocol
+|instruments=Oxygraph-2k, O2k-Fluorometer, O2k-Protocol
-|additional=O2k-Demo, O2k-MultiSensor
+|additional=O2k-Demo, O2k-MultiSensor, MitoFitPublication, SUIT-020, SUIT-020 Fluo mt D033, SUIT-006 Fluo mt D034, SUIT-021 Fluo mt D036, SUIT-021,mt-Membrane
 }}
-:» The OROBOROS MitoLab uses [[Safranin]] O from Sigma (#S2255, 25 g).
-:» More details: [[Talk:MiPNet19.19 Safranin_mt-membranepotential |»Discussion«]].
-== Safranin chemical background ==
-Several substances typically used in SUIT protocols may influence the fluorescence signal from safranin when injected into the O2k-Chamber (for instance colored substances such as cytochrome ''c''). The chemicals used should be tested for this effect in a background run without biological sample, and the necessary corrections be applied.
-=== Substances with an effect on the fluorescence signal of safranin ===
-* ADP (D)
-* Cytochrome ''c'' (c)
-* Succinate (S)
-* Rotenone (Rot)
-* Ascorbate (As)
-* TMPD (Tm)
-=== Substances without an effect on the fluorescence signal of safranin ===
-* Pyruvate (P)
-* Malate (M)
-* Glutamate (G)
-* Digitonin (Dig)
-* Oligomycin (Omy)
-* FCCP (U)
-* Malonic acid (Mna)
-* Antimycin A (Ama)
-* DMSO
-* Ethanol
-* H<sub>2</sub>O<sub>2</sub>
-* H<sub>2</sub>O
-== Reference ==
-* Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542: 163-81. [[Krumschnabel_2014_Methods_Enzymol |»Bioblast pdf«]]