MiPNet18.05 Amplex-Mouse-heart: Difference between revisions
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|topics=Cyt c | |topics=Cyt c | ||
|couplingstates=LEAK, OXPHOS, | |couplingstates=LEAK, OXPHOS, ET | ||
|pathways=N, S, NS | |pathways=N, S, NS | ||
|instruments=Oxygraph-2k, O2k-Fluorometer, O2k-Protocol | |instruments=Oxygraph-2k, O2k-Fluorometer, O2k-Protocol | ||
|additional=O2k-Demo, O2k-MultiSensor, Mt-Preparation, PBI-Shredder | |additional=O2k-Demo, O2k-MultiSensor, Mt-Preparation, PBI-Shredder | ||
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Revision as of 15:19, 13 November 2017
O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate. |
Oroboros (2015-06-16) Mitochondr Physiol Network
Abstract: Eigentler A, Fontana-Ayoub M, Gnaiger E (2015) O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(02):1-6. ยปVersions
The O2k-Fluorescence LED2-Module is an extension of the Oroboros O2k for combining high-resolution respirometry and fluorometric measurements. We applied the Fluo-Sensor Green for measurement of H2O2 production with Amplexยฎ Ultrared in mouse heart mitochondria. Experiments are selected to demonstrate the importance of applying ET-pathway competent substrate states, which are critically evaluated by respiratory performance and considering experimental oxygen levels when measuring mitochondrial H2O2 production.
- >> Product: Oroboros O2k, O2k-Catalogue
- >> Permeabilized muscle fibres
โข O2k-Network Lab: AT Innsbruck Oroboros, AT Innsbruck MitoCom
Labels: MiParea: Respiration
Organism: Mouse
Tissue;cell: Heart
Preparation: Homogenate
Regulation: Cyt c Coupling state: LEAK, OXPHOS, ET Pathway: N, S, NS HRR: Oxygraph-2k, O2k-Fluorometer, O2k-Protocol
O2k-Demo, O2k-MultiSensor, Mt-Preparation, PBI-Shredder