Difference between revisions of "Malonate"
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|info=[[MiPNet09.12]] | |info=[[MiPNet09.12]] | ||
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__TOC__ | __TOC__ | ||
== Application in [[HRR]] == | == Application in [[HRR]] == | ||
=== | {{Chemical_description | ||
::: '''Mna: Malonic acid''' ( CH<sub>2</sub>(COOH)<sub>2</sub>); Sigma M 1296, 5 g, store at R.T.; | |abbr=Mna | ||
|trivial name=Malonate | |||
|complete name=Malonic acid or Propanedioic acid | |||
|chem formula=CH<sub>2</sub>(COOH)<sub>2</sub> | |||
|molar mass=104.06 | |||
|vendor=Sigma-Aldrich | |||
|product number=M1296 | |||
|store at=RT | |||
|sensitivity= | |||
|cas=141-82-2 | |||
|h statements=H318 | |||
|h info=causes serious eye damage | |||
}}<!--::: '''Mna: Malonic acid''' (CH<sub>2</sub>(COOH)<sub>2</sub>); Sigma M 1296, 5 g, store at R.T.; M = 104.06 g·mol<sup>-1</sup>. | |||
::::<span style="color:#8B008B"> '''Recommendation:''' Prepare fresh everyday!</span>--> | |||
:::: '''Recommendation:''' Prepare fresh every day. For info on stability see [https://pubchem.ncbi.nlm.nih.gov/compound/867#section=Use-and-Manufacturing PubChem]. | |||
::: '''Preparation of 2 M stock solution''' (dissolved in | :::: '''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>0) for '''2-mL O2k-chamber''': | ||
::::# Weigh 20.8 mg malonic acid into a Eppendorf tube of appropriate volume (for final volume of 100 µL). | ::::# Weigh 20.8 mg malonic acid into a Eppendorf tube of appropriate volume (for final volume of 100 µL). | ||
| Line 22: | Line 33: | ||
::::# Add H<sub>2</sub>O to a final volume of 100 µL. | ::::# Add H<sub>2</sub>O to a final volume of 100 µL. | ||
::: '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | :::» '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | ||
::::* Titration volume ('''2-mL O2k-chamber'''): 5 µL using a 25 µL syringe. | |||
::::* Final concentration: 5 mM. | |||
:::: '''Preparation of 0.5 M stock solution''' (dissolved in H<sub>2</sub>0) for '''0.5-mL O2k-chamber''': | |||
::::# Take 50 µL of the 2 M stock solution. | |||
::::# Add 150 µL H<sub>2</sub>O. | |||
:::» '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | |||
::::* Titration volume: 5 µL using a 25 µL syringe | ::::* Titration volume ('''0.5-mL O2k-chamber'''): 5 µL using a 25 µL syringe. | ||
::::* Final concentration: 5 mM. | ::::* Final concentration: 5 mM. | ||
=== Malonic acid and malonyl-CoA synthase === | === Malonic acid and malonyl-CoA synthase === | ||
| Line 33: | Line 56: | ||
:::: Reference | :::: Reference | ||
::::* Witkowski A, Thweatt J, Smith S (2011) Mammalian ACSF3 protein is a malonyl-CoA synthetase that supplies the chain extender units for mitochondrial fatty acid synthesis. J Biol Chem 286:33729-36. - [[Witkowski 2011 J Biol Chem |»Bioblast link«]] | ::::* Witkowski A, Thweatt J, Smith S (2011) Mammalian ACSF3 protein is a malonyl-CoA synthetase that supplies the chain extender units for mitochondrial fatty acid synthesis. J Biol Chem 286:33729-36. - [[Witkowski 2011 J Biol Chem |»Bioblast link«]] | ||
{{MitoPedia topics | |||
|mitopedia topic=Inhibitor | |||
}} | |||
Latest revision as of 11:12, 10 June 2021
- high-resolution terminology - matching measurements at high-resolution
Malonate
Description
Malonate (malonic acid) is a competitive inhibitor of succinate dehydrogenase (Complex II). Malonate is a substrate of malonyl-CoA synthase.
Abbreviation: Mna
Reference: MiPNet09.12
Application in HRR
- Mna: Malonate (Malonic acid or Propanedioic acid; CH2(COOH)2), Sigma-Aldrich: M1296, store at RT, CAS: 141-82-2, M = 104.06 g·mol-1
- Hazard statements: H318; causes serious eye damage
- Mna: Malonate (Malonic acid or Propanedioic acid; CH2(COOH)2), Sigma-Aldrich: M1296, store at RT, CAS: 141-82-2, M = 104.06 g·mol-1
- Recommendation: Prepare fresh every day. For info on stability see PubChem.
- Preparation of 2 M stock solution (dissolved in H20) for 2-mL O2k-chamber:
- Weigh 20.8 mg malonic acid into a Eppendorf tube of appropriate volume (for final volume of 100 µL).
- Dissolve in 85 µL 5 M KOH and check pH.
- Titrate small volumes (2 µL) of 5 M KOH until you reach a pH of 6.0 (pH of 7.0 would be ideal, however the pH jumps from pH 6.0 to pH 10.0 with only very small excess KOH).
- Add H2O to a final volume of 100 µL.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 5 µL using a 25 µL syringe.
- Final concentration: 5 mM.
- Preparation of 0.5 M stock solution (dissolved in H20) for 0.5-mL O2k-chamber:
- Take 50 µL of the 2 M stock solution.
- Add 150 µL H2O.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (0.5-mL O2k-chamber): 5 µL using a 25 µL syringe.
- Final concentration: 5 mM.
Malonic acid and malonyl-CoA synthase
Communicated by Garcia-Souza Luiz F 2016-11-11
- Activity of mt-malonyl-CoA synthase (Witkowski et al 2011) in a mt-preparation may cause stimulation of respiration after titration of malonate (unpublished observation).
- Reference
- Witkowski A, Thweatt J, Smith S (2011) Mammalian ACSF3 protein is a malonyl-CoA synthetase that supplies the chain extender units for mitochondrial fatty acid synthesis. J Biol Chem 286:33729-36. - »Bioblast link«
- Reference
MitoPedia topics: Inhibitor
