Difference between revisions of "Malonate"
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{{MitoPedia | {{MitoPedia | ||
|abbr=Mna | |abbr=Mna | ||
|description='''Malonate''' (malonic acid) is a competitive inhibitor of [[ | |description='''Malonate''' (malonic acid) is a competitive inhibitor of [[succinate dehydrogenase]] ([[Complex II]]). Malonate is a substrate of [[malonyl-CoA synthase]]. | ||
|info=[[MiPNet09.12]] | |info=[[MiPNet09.12]] | ||
}} | }} | ||
{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Inhibitor | |mitopedia topic=Inhibitor | ||
}} | }} | ||
__TOC__ | |||
== Application in [[HRR]] == | == Application in [[HRR]] == | ||
'''Mna: | === Preparation and titration === | ||
::: '''Mna: Malonic acid''' ( CH<sub>2</sub>(COOH)<sub>2</sub>); Sigma M 1296, 5 g, store at R.T.; FW = 104.06. | |||
<span style="color:#8B008B"> '''Recommendation:''' Prepare fresh everyday!</span> | |||
'''Preparation of 2 M stock solution''' (dissolved in H20): | ::: '''Preparation of 2 M stock solution''' (dissolved in H20): | ||
:: | ::::# Weigh 20.8 mg malonic acid into a Eppendorf tube of appropriate volume (for final volume of 100 µL). | ||
:: | ::::# Dissolve in 85 µL 5 M KOH and check pH. | ||
:: | ::::# Titrate small volumes (2 µL) of 5 M KOH until you reach a pH of 6.0 (pH of 7.0 would be ideal, however the pH jumps from pH 6.0 to pH 10.0 with only very small excess KOH). | ||
:: | ::::# Add H<sub>2</sub>O to a final volume of 100 µL. | ||
:: | |||
::: '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | |||
::::* Titration volume: 5 µL using a 25 µL syringe (2 mL O2k-chamber). | |||
::::* Final concentration: 5 mM. | |||
=== Malonic acid and malonyl-CoA synthase === | |||
Communicated by [[Garcia-Souza LF]] 2016-11-11 | |||
:::: Activity of mt-[[malonyl-CoA synthase]] (Witkowski et al 2011) in a mt-preparation may cause stimulation of respiration after titration of malonate (unpublished observation). | |||
:: | :::: Reference | ||
::* | ::::* Witkowski A, Thweatt J, Smith S (2011) Mammalian ACSF3 protein is a malonyl-CoA synthetase that supplies the chain extender units for mitochondrial fatty acid synthesis. J Biol Chem 286:33729-36. - [[Witkowski 2011 J Biol Chem |»Bioblast link«]] |
Revision as of 13:07, 17 March 2019
Description
Malonate (malonic acid) is a competitive inhibitor of succinate dehydrogenase (Complex II). Malonate is a substrate of malonyl-CoA synthase.
Abbreviation: Mna
Reference: MiPNet09.12
MitoPedia topics:
Inhibitor
Application in HRR
Preparation and titration
- Mna: Malonic acid ( CH2(COOH)2); Sigma M 1296, 5 g, store at R.T.; FW = 104.06.
Recommendation: Prepare fresh everyday!
- Preparation of 2 M stock solution (dissolved in H20):
- Weigh 20.8 mg malonic acid into a Eppendorf tube of appropriate volume (for final volume of 100 µL).
- Dissolve in 85 µL 5 M KOH and check pH.
- Titrate small volumes (2 µL) of 5 M KOH until you reach a pH of 6.0 (pH of 7.0 would be ideal, however the pH jumps from pH 6.0 to pH 10.0 with only very small excess KOH).
- Add H2O to a final volume of 100 µL.
- O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 5 µL using a 25 µL syringe (2 mL O2k-chamber).
- Final concentration: 5 mM.
Malonic acid and malonyl-CoA synthase
Communicated by Garcia-Souza LF 2016-11-11
- Activity of mt-malonyl-CoA synthase (Witkowski et al 2011) in a mt-preparation may cause stimulation of respiration after titration of malonate (unpublished observation).
- Reference
- Witkowski A, Thweatt J, Smith S (2011) Mammalian ACSF3 protein is a malonyl-CoA synthetase that supplies the chain extender units for mitochondrial fatty acid synthesis. J Biol Chem 286:33729-36. - »Bioblast link«
- Reference