Difference between revisions of "Malonate"

Revision as of 13:07, 17 March 2019

high-resolution terminology - matching measurements at high-resolution

Malonate

Description

Malonate (malonic acid) is a competitive inhibitor of succinate dehydrogenase (Complex II). Malonate is a substrate of malonyl-CoA synthase.

Abbreviation: Mna

Reference: MiPNet09.12

MitoPedia topics: Inhibitor

Application in HRR

Preparation and titration

Mna: Malonic acid ( CH₂(COOH)₂); Sigma M 1296, 5 g, store at R.T.; FW = 104.06.

Recommendation: Prepare fresh everyday!

Preparation of 2 M stock solution (dissolved in H20):

Weigh 20.8 mg malonic acid into a Eppendorf tube of appropriate volume (for final volume of 100 µL).
Dissolve in 85 µL 5 M KOH and check pH.
Titrate small volumes (2 µL) of 5 M KOH until you reach a pH of 6.0 (pH of 7.0 would be ideal, however the pH jumps from pH 6.0 to pH 10.0 with only very small excess KOH).
Add H₂O to a final volume of 100 µL.

O2k manual titrations MiPNet09.12 O2k-Titrations

Titration volume: 5 µL using a 25 µL syringe (2 mL O2k-chamber).
Final concentration: 5 mM.

Malonic acid and malonyl-CoA synthase

Communicated by Garcia-Souza LF 2016-11-11

Activity of mt-malonyl-CoA synthase (Witkowski et al 2011) in a mt-preparation may cause stimulation of respiration after titration of malonate (unpublished observation).

Reference

Witkowski A, Thweatt J, Smith S (2011) Mammalian ACSF3 protein is a malonyl-CoA synthetase that supplies the chain extender units for mitochondrial fatty acid synthesis. J Biol Chem 286:33729-36. - »Bioblast link«

@@ Line 8: / Line 8: @@
 }}
 __TOC__
-== Application in [[HRFR]] ==
+== Application in [[HRR]] ==
 === Preparation and titration ===
@@ Line 17: / Line 17: @@
 ::: '''Preparation of 2 M stock solution''' (dissolved in H20):
-::::# Weigh 20.8 mg malonic acid into a Eppendorf tube of appropriate volume (for final volume of 100 µl).
+::::# Weigh 20.8 mg malonic acid into a Eppendorf tube of appropriate volume (for final volume of 100 µL).
-::::# Dissolve in 85 µl 5 M KOH and check pH.
+::::# Dissolve in 85 µL 5 M KOH and check pH.
-::::# Titrate small volumes (2 µl) of 5 M KOH until you reach a pH of 6.0 (pH of 7.0 would be ideal, however the pH jumps from pH 6.0 to pH 10.0 with only very small excess KOH).
+::::# Titrate small volumes (2 µL) of 5 M KOH until you reach a pH of 6.0 (pH of 7.0 would be ideal, however the pH jumps from pH 6.0 to pH 10.0 with only very small excess KOH).
-::::# Add H<sub>2</sub>O to a final volume of 100 µl.
+::::# Add H<sub>2</sub>O to a final volume of 100 µL.
 ::: '''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
-::::* Titration volume: 5 µl using a 25 µl syringe (2 ml O2k-chamber).
+::::* Titration volume: 5 µL using a 25 µL syringe (2 mL O2k-chamber).
 ::::* Final concentration: 5 mM.
 ===  Malonic acid and malonyl-CoA synthase ===
-  Communicated by [[Garcia e Souza LF]] 2016-11-11
+  Communicated by [[Garcia-Souza LF]] 2016-11-11
 :::: Activity of mt-[[malonyl-CoA synthase]] (Witkowski et al 2011) in a mt-preparation may cause stimulation of respiration after titration of malonate (unpublished observation).
 :::: Reference
 ::::* Witkowski A, Thweatt J, Smith S (2011) Mammalian ACSF3 protein is a  malonyl-CoA synthetase that supplies the chain extender units for  mitochondrial fatty acid synthesis. J Biol Chem 286:33729-36. - [[Witkowski 2011 J Biol Chem |»Bioblast link«]]