Difference between revisions of "Karavyraki 2022 BEC"
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ORC'''ID''': Karavyraki Marilena, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3647-5895]] Gnaiger Erich, [[File:ORCID.png|20px|link=https://orcid.org/0000-0001-9854-5161]] Porter Richard K | ORC'''ID''': Karavyraki Marilena, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3647-5895]] Gnaiger Erich, [[File:ORCID.png|20px|link=https://orcid.org/0000-0001-9854-5161]] Porter Richard K | ||
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== Data availibility == | == Data availibility == | ||
:::: Raw data used for this manuscript is available upon reasonable request to the corresponding author | :::: Raw data used for this manuscript is available upon reasonable request to the corresponding author | ||
Revision as of 11:14, 11 November 2022
| Karavyraki M, Gnaiger E, Porter RK (2022) Bioenergetics in human tongue pre-cancerous dysplastic oral keratinocytes and squamous cancer cells https://doi.org/10.26124/bec:2022-0011 |
» Bioenerg Commun 2022.11. 
published online 2022-11-04
Karavyraki Marilena, Gnaiger Erich, Porter Richard K (BEC 2022.11) Bioenerg Commun
Abstract: 
https://doi.org/10.26124/bec:2022-0011
In an endeavor to understand the metabolic phenotype behind oral squamous cell carcinomas, we characterized the bioenergetic profile of a human tongue derived cancer cell line (SCC-4 cells) and compared this profile to a pre-cancerous dysplastic oral keratinocyte (DOK) cell line also derived from human tongue. The human SCC-4 cancer cells had greater mitochondrial abundance but lower mitochondrial oxygen consumption rates than DOK cells. The lower oxygen consumption rate in SCC-4 cells can be partially explained by lower NADH-related enzymatic activity and lower mitochondrial complex 1 activity when compared to pre-cancerous DOK cells. In addition, SCC-4 cells have greater extracellular acidification rate (an index of glycolytic flux) when compared to DOK cells. In addition, treatment with recombinant human IL-6 (rhIL-6), known to drive anoikis resistance in SCC-4 cells but not DOK cells, impairs oxygen consumption in SCC-4 but not DOK cells, without affecting mitochondrial abundance. We conclude that SCC-4 cells have a less oxidative phenotype compared to DOK cells and that IL-6 attenuates mitochondrial function in SCC-4 cells while increasing glycolytic flux. • Keywords: oral squamous cancer cells; mitochondria; interleukin 6; dysplastic oral keratinocytes; oxygen consumption • Bioblast editor: Tindle-Solomon L • O2k-Network Lab: AT Innsbruck Oroboros, IE Dublin Porter RK
ORCID: Karavyraki Marilena, 
Gnaiger Erich, Porter Richard K
Data availibility
- Raw data used for this manuscript is available upon reasonable request to the corresponding author
Preprint
Support
- Marie Curie Grant TRACT 721906 H2020-MCSA-ITN 2016; COST Action CA15203 MitoEAGLE (2016-2021). We thank Rafael Moreno-Sanchez for a constructive review of our manuscript.
Labels: MiParea: Respiration
Pathology: Cancer
Organism: Human
Preparation: Permeabilized cells Enzyme: Complex I, Marker enzyme, TCA cycle and matrix dehydrogenases Regulation: Aerobic glycolysis Coupling state: LEAK, ROUTINE, ET Pathway: S, ROX HRR: Oxygraph-2k
Crabtree effect, BEC
