High signal at zero oxygen: Difference between revisions

Revision as of 14:40, 30 October 2020

High signal at zero oxygen

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

Description

'A high signal at zero oxygen' may be observed during zero calibration. The following instructions show how to further localize and possibly solve this technical problem.

Abbreviation: R0

Reference: Zero calibration, OroboPOS-Connector

High zero current

Note: Before sending us the O2k or any spare components, please contact us via our O2k-Open Support system: O2k-Open Support

References:

[1] Cleaning the electrical connections

[2] MiPNet19.18B POS-service

[3] MiPNet06.03 POS-calibration-SOP

[4] MiPNet14.06 Instrumental O2 background

Example: First, the OroboPOS-Connector and the POS were disassembled and disconnected from the main unit. Second, the OroboPOS-Connector was connected to the O2k-Main unit without the OroboPOS. Third, the OroboPOS was connected to the OroboPOS-Connector without membranes. After each step, the O₂ raw signal [V] was recorded for 10 min. The marks were set to the O₂ raw signal [V] to evaluate the zero current.

Figure 1. High zero current test. Upper panel: Zero current is in the range of ±0.00099 V. Lower panel: Zero current is higher than 0.00099 V when the OroboPOS-Connector is connected to the O2k-Main unit and when the OroboPOS is connected to the OroboPOS-Connector, which means that both the OroboPOS-Connector and OroboPOS might have electrical problems.

Keywords

Bioblast links: Oxygen signal - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

Specific

O2k-Procedures

» High signal at zero oxygen

» POS calibration - static

» POS calibration - dynamic

MiPNet O2k-Procedures

» POS-calibration

» O2k-Manual: POS-service

» O2k-cleaning

» O2k-Series G and DatLab 6: Calibration

General

» Oxygen, dioxygen, O₂

» Oxygen calibration - DatLab

» Oxygen solubility

» Oxygen solubility factor

» Oxygen pressure

» Concentration

» Activity

» Pressure - Pascal

» Barometric pressure

» High-resolution respirometry

» OroboPOS

» Polarographic oxygen sensor

» MitoFit Quality Control System

» Sensitivity

Other keyword lists

» Keywords: Concentration and pressure

MitoPedia O2k and high-resolution respirometry: O2k hardware, O2k-Open Support, O2k-Respirometry

Revision as of 14:22, 30 October 2020 (view source) Komlodi Timea (talk \| contribs) No edit summary ← Older edit		Revision as of 14:40, 30 October 2020 (view source) Komlodi Timea (talk \| contribs) No edit summary Newer edit →
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	::::[[File:High zero current test bad exmaple.png\|600px]]		::::[[File:High zero current test bad exmaple.png\|600px]]
	::::'''Figure 1.''' High zero current test. '''Upper panel:''' Zero current is in the range of ±0.00099 V. '''Lower panel:''' Zero current is higher than 0.00099 V when the OroboPOS-Connector is connected to the O2k-Main unit and when the OroboPOS is connected to the OroboPOS-Connector, which means that both the OroboPOS-Connector and OroboPOS might have electrical problems. ~~In order to localize the problem, follow the [[Oxygen_sensor_test#Switch_components_approach]] from step 3.~~		::::'''Figure 1.''' High zero current test. '''Upper panel:''' Zero current is in the range of ±0.00099 V. '''Lower panel:''' Zero current is higher than 0.00099 V when the OroboPOS-Connector is connected to the O2k-Main unit and when the OroboPOS is connected to the OroboPOS-Connector, which means that both the OroboPOS-Connector and OroboPOS might have electrical problems.