Bioblast alert 2011: Difference between revisions
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[[Image:BB-Bioblast.jpg|left|30px|link=Bioblast:About|Bioblast wiki]] | |||
[[File:Bioblast alert.jpg|240px|right|link=Bioblast_alert|Bioblast alert]] | |||
''' | '''[[Bioblast alert|back to "Bioblast alert" main page]]''' | ||
__TOC__ | __TOC__ | ||
=== Bioblast alert 2011(01): 2011-11-11 === | === Bioblast alert 2011(01): 2011-11-11 === | ||
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* A key question on the separation of '''qualitative versus quantitative change of mitochondrial function''' is addressed in a new O2k-Publication. Does training induce mild uncoupling? Are sedentary humans healthy controls? | * A key question on the separation of '''qualitative versus quantitative change of mitochondrial function''' is addressed in a new O2k-Publication. Does training induce mild uncoupling? Are sedentary humans healthy controls? | ||
::[[Pesta_2011_Am J Physiol Regul Integr Comp Physiol|Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol 301: R1078–R1087.]] | ::[[Pesta_2011_Am J Physiol Regul Integr Comp Physiol|Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol 301: R1078–R1087.]] | ||
* [[ | * [[ET capacity]] plays a key role in such analyses, which cannot be achieved quantitatively without careful '''[[Optimum uncoupler concentration|uncoupler titrations]]'''. | ||
* '''[[Basal respiration]]''' - term of the month: a hallmark of high-resolution respirometry is a matching high-resolution terminology. | * '''[[Basal respiration]]''' - term of the month: a hallmark of high-resolution respirometry is a matching high-resolution terminology. | ||
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=== Bioblast alert 2011(02): 2011-11-17 === | === Bioblast alert 2011(02): 2011-11-17 === | ||
* '''[ | * '''[[MiPNet16.02 IOC64|IOC64 - Centre for Cellular and Molecular Biology, Hyderabad, India]]: 2011-12-08'''. | ||
* '''Heart failure and mitochondrial dysfunction'''. Increased [[OXPHOS capacity]] after cold preservation of the human heart – a paradox resolved by a dyscoupling mechanism. | * '''Heart failure and mitochondrial dysfunction'''. Increased [[OXPHOS capacity]] after cold preservation of the human heart – a paradox resolved by a dyscoupling mechanism. | ||
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* '''[[Tissue storage]]''' on ice is possible for limited periods of time (several hours) with preservation of mitochondrial respiratory function, as evaluated with [[SUIT protocol]]s. | * '''[[Tissue storage]]''' on ice is possible for limited periods of time (several hours) with preservation of mitochondrial respiratory function, as evaluated with [[SUIT protocol]]s. | ||
* '''[[Uncoupled]], [[ | * '''[[Uncoupled]], [[noncoupled]], [[dyscoupled]]''' - term of the week: a hallmark of high-resolution respirometry is a matching high-resolution terminology. | ||
::[[Talk:Uncoupled respiration|Evaluate an example of low-resolution terminology]]: '''''[[Dyscoupled respiration|Uncoupling]]''' was studied in stressed cells by measurement of coupled respiration, evaluation of '''[[LEAK|uncoupled]]''' respiration after inhibition with oligomycin, and FCCP titration to quantify '''[[ | ::[[Talk:Uncoupled respiration|Evaluate an example of low-resolution terminology]]: '''''[[Dyscoupled respiration|Uncoupling]]''' was studied in stressed cells by measurement of coupled respiration, evaluation of '''[[LEAK respiration|uncoupled]]''' respiration after inhibition with oligomycin, and FCCP titration to quantify '''[[noncoupled|uncoupled]]''' respiration''. | ||
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=== Bioblast alert 2011(03): 2011-11-24 === | === Bioblast alert 2011(03): 2011-11-24 === | ||
* '''Fluorometry combined with high-resolution respirometry''': Light guide inserted into the chamber through a black PEEK stopper<ref>[[Anderson 2011 Am J Physiol Heart Circ Physiol|Anderson EJ, Rodriguez E, Anderson CA, Thayne K, Chitwood WR, Kypson AP (2011) Increased propensity for cell death in diabetic human heart is mediated by mitochondrial-dependent pathways. Am J Physiol Heart Circ Physiol 300: H118- | * '''Fluorometry combined with high-resolution respirometry''': Light guide inserted into the chamber through a black PEEK stopper<ref>[[Anderson 2011 Am J Physiol Heart Circ Physiol|Anderson EJ, Rodriguez E, Anderson CA, Thayne K, Chitwood WR, Kypson AP (2011) Increased propensity for cell death in diabetic human heart is mediated by mitochondrial-dependent pathways. Am J Physiol Heart Circ Physiol 300:H118-24.]]</ref>, or optical probe operated through the O2k-glass window<ref>[[Hickey 2012 J Comp Physiol B|Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2012) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (''Hemiscyllum ocellatum''). J Comp Physiol B 182:91-100. ]]</ref>. | ||
<references/> | <references/> | ||
*'''[[Talk:Fluorometry|Fluorometry versus fluorimetry]]''' - term of the week. >>>New '''[[ | *'''[[Talk:Fluorometry|Fluorometry versus fluorimetry]]''' - term of the week. >>>New '''[[:MitoPedia: Fluorometry]]''' - high-resolution terminology. | ||
=== Bioblast alert 2011(04): 2011-12-24 === | === Bioblast alert 2011(04): 2011-12-24 === | ||
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* [[Bioblast editorial board]] - [[Talk:Bioblast editorial board|Why a wiki editorial board?]] | * [[Bioblast editorial board]] - [[Talk:Bioblast editorial board|Why a wiki editorial board?]] | ||
* '''[[SUIT protocol]]s for HRR: from intact cells to permeabilized muscle fibres''': | * '''[[SUIT protocol]]s for HRR: from intact cells to permeabilized muscle fibres''': | ||
::[[Pesta 2012 Methods Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small | ::[[Pesta 2012 Methods Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58.]] | ||
[[Category:Gentle Science]] | [[Category:Gentle Science]] |
Latest revision as of 12:24, 25 November 2020
back to "Bioblast alert" main page
Bioblast alert 2011(01): 2011-11-11
- A key question on the separation of qualitative versus quantitative change of mitochondrial function is addressed in a new O2k-Publication. Does training induce mild uncoupling? Are sedentary humans healthy controls?
- ET capacity plays a key role in such analyses, which cannot be achieved quantitatively without careful uncoupler titrations.
- Basal respiration - term of the month: a hallmark of high-resolution respirometry is a matching high-resolution terminology.
Bioblast alert 2011(02): 2011-11-17
- Heart failure and mitochondrial dysfunction. Increased OXPHOS capacity after cold preservation of the human heart – a paradox resolved by a dyscoupling mechanism.
- Tissue storage on ice is possible for limited periods of time (several hours) with preservation of mitochondrial respiratory function, as evaluated with SUIT protocols.
- Uncoupled, noncoupled, dyscoupled - term of the week: a hallmark of high-resolution respirometry is a matching high-resolution terminology.
- Evaluate an example of low-resolution terminology: Uncoupling was studied in stressed cells by measurement of coupled respiration, evaluation of uncoupled respiration after inhibition with oligomycin, and FCCP titration to quantify uncoupled respiration.
Bioblast alert 2011(03): 2011-11-24
- Fluorometry combined with high-resolution respirometry: Light guide inserted into the chamber through a black PEEK stopper[1], or optical probe operated through the O2k-glass window[2].
- ↑ Anderson EJ, Rodriguez E, Anderson CA, Thayne K, Chitwood WR, Kypson AP (2011) Increased propensity for cell death in diabetic human heart is mediated by mitochondrial-dependent pathways. Am J Physiol Heart Circ Physiol 300:H118-24.
- ↑ Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2012) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (Hemiscyllum ocellatum). J Comp Physiol B 182:91-100.
- Fluorometry versus fluorimetry - term of the week. >>>New MitoPedia: Fluorometry - high-resolution terminology.
Bioblast alert 2011(04): 2011-12-24
- Congratulations to Dr. Dominik Pesta, for receiving his degree of a PhD at the Medical University of Innsbruck! (Jan 2012)
- Bioblast editorial board - Why a wiki editorial board?
- SUIT protocols for HRR: from intact cells to permeabilized muscle fibres: