Octanoylcarnitine: Difference between revisions
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|description='''Octanoylcarnitine''' is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to [[carnitine]], frequently applied as a substrate for [[fatty acid oxidation]] (FAO) in [[mitochondrial preparations]]. | |description='''Octanoylcarnitine''' is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to [[carnitine]], frequently applied as a substrate for [[fatty acid oxidation]] (FAO) in [[mitochondrial preparations]]. | ||
|info=[http://www.jbc.org/content/256/16/8371.full.pdfย ''pdf''] | |info=[http://www.jbc.org/content/256/16/8371.full.pdfย ''pdf''] | ||
}} | }} | ||
:ยป [[Fatty acid oxidation]] | :ยป [[Fatty acid oxidation]] | ||
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::::* Titration volume: 10 ยตL using a 25 ยตL syringe. | ::::* Titration volume: 10 ยตL using a 25 ยตL syringe. | ||
::::* Final concentration: 0.5 mM in [[MiR05-Kit|MiR05]] or [[MiR06]]. Optimum Oct concentrations have to be evaluated, particularly in media with BSA concentrations different from MiR05. | ::::* Final concentration: 0.5 mM in [[MiR05-Kit|MiR05]] or [[MiR06]]. Optimum Oct concentrations have to be evaluated, particularly in media with BSA concentrations different from MiR05. | ||
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|mitopedia topic=Substrate and metabolite | |||
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Revision as of 12:01, 3 September 2020
Description
Octanoylcarnitine is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to carnitine, frequently applied as a substrate for fatty acid oxidation (FAO) in mitochondrial preparations.
Abbreviation: Oct
Reference: pdf
Application in HRR
- Oct: Octanoylcarnitine ((ยฑ)-Octanoylcarnitine chloride, C15H30NO4Cl); APExBIO Technology, Cat. No. B6371, 50 mg, store at -20ยฐC; FW = 323.86.
- Alternative: (ยฑ)-Octanoylcarnitine chloride, BioTrend, Cat. No. BS0136, 50 mg, store desiccate at RT; FW = 323.86.
- TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at RT, desiccate); FW = 323.85.
- General tips: For obtaining a higher solubility, warm the tube at 37 โ and shake it in the ultrasonic bath for a while.
- Alternative: (ยฑ)-Octanoylcarnitine chloride, BioTrend, Cat. No. BS0136, 50 mg, store desiccate at RT; FW = 323.86.
- Preparation of 100 mM stock solution (dissolved in H2O or DMSO, can differ from batch to batch - check Certificate of Analysis):
- Weigh 50 mg of octanoylcarnitine and dissolve in 1543 ยตL H2O or DMSO.
- Divide into 0.2 mL portions.
- Store at -20 ยฐC.
- O2k manual titrations into 2 mL O2k-chamber
MitoPedia topics:
Substrate and metabolite