MiPNet23.11 IOC137 Innsbruck AT

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MiPNet23.11 IOC137 Innsbruck AT

Publications in the MiPMap
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Innsbruck AT, 2018 Dec 10-12. MitoFit Coaching Days - Basic IOC137.

»

Oroboros (2018-12-10) Mitochondr Physiol Network

Abstract:
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MitoFit Coaching Days - Basic. Innsbruck, Austria; 2018 Dec 10-12.


O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit

General information

TRANSMIT
REMIX
MitoFit Coaching Days - Basic provide a fundamental introduction to high-resolution respirometry with the Oroboros O2k. Hands-on sessions range from OroboPOS technical service to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols.
Early stage researchers (ESR´s) during their Marie Skłodowska-Curie project - TRANSMIT or REMIX will be trained at the O2k-Workshop on high-resolution respirometry:
» http://remix-itn.eu/
» https://www.transmit-project.eu


» Information on travel and venue: IOC Innsbruck.
Please bring your labcoat with you. Thank you!

Feedback

11 feedback forms returned
  • Instructors - so patient! So kind! Really good at explaining things over and over again.
  • Thank you to all involved including other participants who were also kind and keen to learn and share.
  • Great company and team!
Question Rating (average)
In how far were your interests covered in the workshop? Please rate from 1 (not covered) to 5 (all interests covered). 4.27
Balance between practical “hands-on” work and “theoretical” explanations: Please rate from 1 (too much “hands-on”) to 5 (too much theory). 3
Presentation of lectures: Please rate from 1 (not understandable) to 5 (very understandable). a. in terms of content: 4.18
Presentation of lectures: Please rate from 1 (not understandable) to 5 (very understandable). b. in terms of language: 4.36
Level of tutoring during the “hands-on” work: Please rate from 1 (not enough tutoring, I felt left alone) to 5 (too much tutoring, I would have liked to work more independently). 3.14
Quality of tutoring: Please rate from 1 (poor) to 5 (excellent). 4.82
Intensity of the O2k-Course: Please rate from 1 (I would have liked to do and hear more) to 5 (I felt overwhelmed). 2.77
Your overall rating of the course? Please rate from 1 (poor) to 5 (excellent). 4.32


Registration

closed

Tutors, lecturers, and managers



Programme

Monday 10th Topic Room
08:15 Welcome - Get-together: Introduction of participants and their research interests ORO-MitoFit Lab
08:30 OroboPOS service and O2k instrumental setup - overview with videoclips ORO-MitoFit Lab
09:30 Hands-on: OroboPOS service and O2k instrumental setup ORO-MitoFit Lab
10:10 Hands-on: DatLab 7 ORO-MitoFit Lab
10:30 Coffee
11:00 Instrumental quality control 1: oxygen calibration ORO-MitoFit Lab
11:30 Hands-on: Instrumental quality control 1: oxygen calibration

DL-Protocol: O2k-cleaning before use

DL-Protocol: O2 calibration air

ORO-MitoFit Lab
12:00 Lunch break
13:00 Hands-on:Instrumental quality control 1: oxygen calibration (continuation)

DL-Protocol: O2 calibration air

ORO-MitoFit Lab
13:30 Instrumental quality control 2: Instrumental O2 background – overview with videoclips ORO-MitoFit Lab
14:00 Hands-on:Instrumental quality control 2: Instrumental O2 background

DL-Protocol: Instrumental O2 background TiP2k

ORO-MitoFit Lab
15:00 Coffee
15:30-18:00 Hands-on: Instrumental quality control 1 and 2. DatLab analysis. ORO-MitoFit Lab
Tuesday 11th Topic Room
09:00 Hands-on: Instrumental quality control 1: oxygen calibration

DL-Protocol: O2k-cleaning before use

DL-Protocol: O2 calibration air

ORO-MitoFit Lab
09:15 Application of the O2k Series H ORO-MitoFit Lab
09:30 Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. SUIT reference protocol: RP1&RP2 ORO-MitoFit Lab
10:00 Coffee
10:30 Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.

DL-Protocol: SUIT-1_O2_pce_D03.DLP

DL-Protocol: SUIT-2 O2 pce D07.DLP

ORO-MitoFit Lab
11:45 Hands-on: O2k-cleaning after use

DL-Protocol: O2k-cleaning after use

ORO-MitoFit Lab
12:00 Lunch break
13:00 Hands-on: O2k-cleaning after use

DL-Protocol: O2k-cleaning after use

ORO-MitoFit Lab
13:30 Introduction to DatLab analysis ORO-MitoFit Lab
15:00 Coffee
15:30-18:00 Individual DatLab analysis + summary ORO-MitoFit Lab
Wednesday 12th Topic Room
09:00 MitoFit proficiency test:

Hands-on: Instrumental quality control 1: oxygen calibration

DL-Protocol: O2k-cleaning before use

DL-Protocol: O2 calibration air

ORO-MitoFit Lab
10:00 Coffee
10:30 MitoFit proficiency test:

Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.

DL-Protocol: SUIT-1_O2_pce_D03.DLP

DL-Protocol: SUIT-2 O2 pce D07.DLP

ORO-MitoFit Lab
11:45 Hands-on: O2k-cleaning after use

DL-Protocol: O2k-cleaning after use

ORO-MitoFit Lab
12:00 Lunch break
13:00 Hands-on: O2k-cleaning after use

DL-Protocol: O2k-cleaning after use

ORO-MitoFit Lab
13:15 DatLab analysis ORO-MitoFit Lab
14:00 DatLab analysis summary ORO-MitoFit Lab
15:00 Coffee
15:30 The Bioblast wiki, O2k-Network and MITOEAGLE MiPArt
16:15 Feedback & conclusions MiPArt
17:00 Farewell activity MiPArt

Participants

Participant Institution
Participant Barlow Jonathan UK Birmingham Lai YC, University of Birmingham, UK
Participant Chakrabarti Lisa UK Nottingham Chakrabarti L, University of Nottingham, UK
Participant Gnaiger Carolina AT Innsbruck Oroboros, Innsbruck, AT
Participant Ho Dieu Hien DE Cologne Trifunovic A, Institute of Mitocondiral Disease and Ageing, Cologne, DE
Participant Jedlicka Jan CZ Pilsen Kuncova J, Charles University, Plzne, CZ
Participant Kapferer Werner AT Innsbruck Jansen-Duerr P, University of Innsbruck, AT
Sabine Kranewitter Sabine AT Innsbruck Oroboros, Innsbruck, AT
Participant Maciej Sarah DE Cologne Trifunovic A, Institute of Mitocondiral Disease and Ageing, Cologne, DE
Participant Maseko Tumisang Edward CZ Hradec Kralove Cervinkova Z, Charles University, Hradec Kralove, CZ
Participant Oliveira Ben UK Leicester Watson E, Kings College Hospital, London, UK
Participant Saharnaz Sarlak FR Bordeaux Rossignol R, University of Bordeaux, Bordeaux, FR
Participant Thapa Maheshwor AT Salzburg Sperl W, Biocrates Life Sciences AG, Innsbruck, AT
Participant Ton Riccardo AU Sydney Griffith SC, Macquarie University, Sydney, AU
Participant Zdrazilova Lucie CZ Prague Zeman J, Charles University, Prague, CZ

Impressions of the IOC137


Contact

instruments@oroboros.at


Oroboros Instruments
High-resolution respirometry
Schöpfstrasse 18
A-6020 Innsbruck, Austria
Tel: +43 512 566796
Fax: +43 512 566796 20
Mitochondria and cell research

Project support

Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
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IOC recommended reading

» IOC recommended reading
» MitoPedia: Respiratory states
OXPHOS ROUTINE ETS LEAK ROX


MitoGlobal
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.


Labels:






ORO, IOC, 2018, MitoFit, TRANSMIT, MitoGlobal