Minuzzi LM

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MiPsociety
COST Action CA15203 MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping


 

Minuzzi LM


MitoPedia topics: EAGLE 

COST: Member COST WG1: WG1


Name Guerra Minuzzi Luciele, Ms
Institution Center for Neuroscience and Cell Biology,

Faculty of Medicine, University of Coimbra, PT

Address Rua Larga, 3004-504
City Coimbra
State/Province
Country Portugal
Email lucielegm@gmail.com
Weblink
O2k-Network Lab


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Publications

 PublishedReference
Gnaiger 2019 MitoFit Preprint Arch2019Gnaiger E, Aasander Frostner E, Abdul Karim N, Abdel-Rahman EA, Abumrad NA, Acuna-Castroviejo D, Adiele RC, et al (2019) Mitochondrial respiratory states and rates. MitoFit Preprint Arch doi:10.26124/mitofit:190001.v6.

Abstracts

 PublishedReference
Minuzzi 2019 MiPschool Coimbra2019
MiPsociety
A pilot study on mitochondrial function of mouse skin using high-resolution respirometry.
Work Plan summary
I had completed my Ph.D. in Sport Sciences – Field of Physical Activity and Health in June 2017, at the University of Coimbra, under the supervision of Ana Maria Miranda Botelho Teixeira. Since I started my Ph.D. I performed a series of investigations into the effect of lifelong physical training on the immunosenescence markers of master athletes.
In October 2017, as a postdoc research at the University of Campinas – Brazil and under supervision of Jose Rodrigo Pauli, I intended to assess the effects of physical exercise on immunosenescence and to ascertain whether the administration of metformin may contribute in some way to a delay in immune deficiency.
In 2019, I moved to Coimbra to work at the Center for Neurosciences and Cell Biology (CNC.IBILI) at the University of Coimbra, Portugal. Actually, as a postdoc member of Obesity, Diabetes and Complications Group at the CNC.IBILI, under supervision of Eugenia Carvalho, I started a new research subject to understand the role and the interaction between the immune system and metabolism. We are interested in understanding the regulation of processes of wound healing impairment and the disruption of tissue repair under diabetes conditions and the cross-talk with immune cells. So, I began my journey studying the high-resolution respirometry protocols.
High-resolution respirometry allows accurate measurements of many aspects of tissue mitochondrial function including phosphorylation, electron transport capacity, coupling and respiratory control with varying substrate supply. Multiple substrate-uncoupler-inhibitor tritation (SUIT) protocols for analysis of oxidative phosphorylation (OXPHOS) improve our understanding of mitochondrial respiratory control. To best of our knowledge, different respiratory states have not yet been addressed in the context of diabetic wounds in permeabilized skin. Therefore, we are working on two SUIT protocols to quantify mitochondrial oxidative capacity in skin in murine model using high-resolution respirometry with a measurement regimen optimized for tissue preparation and substrate supply. Evaluation of mitochondrial function in skin biopsies by respirometry may be a bio-sensitive marker of the progression of diabetic foot wound. For this reason, the research of new therapies that seek to understand the mechanisms associated with the development of the wound is very important to improve the quality of life of the patients, decreasing economic costs of chronic wounds and to alleviate psychological sequelae for the patients.
Altogether, this training course appears to be an ideal opportunity to improve my skills on a high-resolution respirometer O2k and gain hands-on experience. The updated information will be fundamental to the instrumental quality control on my data and to learn more about coupling states and rates in mitochondrial preparations that will be helpful to understanding our results.

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