- high-resolution terminology - matching measurements at high-resolution
SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-
Description
Abbreviation: RLE(SD)
Reference: A Stadlmann 2006 Cell Biochem Biophys
MitoPedia concepts:
SUIT protocol,
SUIT A
- SUIT-category: RLE(SD)
- SUIT protocol pattern: complex coupling control protocol (CCP)
,3L(S),4E(S)-5ROX(S),6ROX.jpg)
Coupling control protocol
- 1R;2SD;3Omy;4U;5Rot;6Ama
| Step | Respiratory state | Pathway control | Comment |
|---|---|---|---|
| 1R | ROUTINE | ce | pce without substrate are in a state of ROX. |
| 2SD | R+SP | ROUTINE (R) + S (SP) | pce are in state SP. |
| 3Omy | L+SL | LEAK (L) + S (SL) | ce and pce are in the LEAK state. |
| 4U | E+SE | ETS (E) + S (SE) | ce and pce are in the ETS state. |
| 5Rot | ROX+SE | ROX+S (SE) | ce are in the ROX state, pce are in the ETS state. |
| 6Ama | ROXAma | ROX |
- 1R 1S .. versus 1R 2Omy 3U 4S .. and respiration medium
- Intact cells & succinate: ADP is not permeable through the plasma membrane of intact cells, and this is the case for succinate in many cell types (Steinlechner-Maran 1997 Transplantation). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability (Stadlmann_2006_Cell Biochem Biophys).
