SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-

From Bioblast
Jump to: navigation, search
Bioblasts - Richard Altmann and MiPArt by Odra Noel
MitoPedia         Terms and abbreviations         Concepts and methods         Oroboros O2k         MiP and biochemistry         Preprints and history



MitoPedia

SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-

Description

Ce1;ce2SD;ce3Omy;ce4U;ce5Rot;ce6Ama.png

Abbreviation: FNS(Oct,PGM)

Reference: B SUIT-003 Stadlmann 2006 Cell Biochem Biophys


O2k-Application: O2

SUIT protocol pattern: complex cell coupling control protocol ce1;ce2SD;ce3Omy;ce4U;ce5Rot;ce6Ama
1R,2R(SD),3L(S),4E(S)-5ROX(S),6ROX.jpg

Stadlmann 2006 Cell Biochem Biophys Fig1B-updated.jpg

Coupling control protocol

  • 1ce;2ceSD;3ceOmy;4ceU;5ceRot;6ceAma
Step Respiratory state Pathway control Comment
ce1 ROUTINE Iiving cells (ce) Dead cells (dce) without substrate are in a state of ROX.
ce2SD R+SP ROUTINE (R) + S (SP) dce with functional mitochondria are in state SP.
ce3Omy L+SL LEAK (L) + S (SL) ce and dce are in the LEAK state.
ce4U E+SE ET-pathway (E) + S (SE) ce and dce are in the ET state.
ce5Rot ROX+SE ROX+S (SE) ce are in the ROX state, dce are in the ET state.
ce6Ama ROXAma ROX


  • ce1;ce1S .. versus ce1;ce2Omy;ce3U;ce4S .. and respiration medium
Living cells & succinate: ADP is not permeable through the plasma membrane of living cells, and this is the case for succinate in many cell types (Steinlechner-Maran 1997 Transplantation). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability (Stadlmann_2006_Cell Biochem Biophys).


MitoPedia concepts: SUIT protocol, SUIT B