SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-

high-resolution terminology - matching measurements at high-resolution

SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-

Description

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Abbreviation: CCP(S)02_R,1SD,2Omy,3U-4Rot,5Ama

Reference: A Stadlmann 2006 Cell Biochem Biophys

MitoPedia concepts: SUIT protocol, SUIT A 

SUIT-catg: CCP(S)

SUIT protocol pattern: complex coupling control protocol (CCP)

Mark names in DatLab: CCP(S)02

DatLab-Analysis template: in prep.: CCP(S)02.xlsx

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Coupling control protocol

Step	Respiratory state	Pathway control	Comment
R	ROUTINE	ce	pce without substrate are in a state of ROX.
1SD	R+SP	ROUTINE (R) + S (SP)	pce are in state SP.
2Omy	L+SL	LEAK (L) + S (SL)	ce and pce are in the LEAK state.
3U	E+SE	ETS (E) + S (SE)	ce and pce are in the ETS state.
4Rot	ROX+SE	ROX+S (SE)	ce are in the ROX state, pce are in the ETS state.
5Ama	ROXAma	ROX

• R 1S .. versus R 1Omy 2U 3S .. and respiration medium

Intac cells & succinate: ADP is not permeable through the plasma membrane of intact cells, and this is the case for succinate in many cell types (Steinlechner-Maran 1997 Transplantation). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability (Stadlmann_2006_Cell Biochem Biophys).