Difference between revisions of "Octanoylcarnitine"

Revision as of 09:26, 5 September 2015

high-resolution terminology - matching measurements at high-resolution

Octanoylcarnitine

Description

Octanoylcarnitine is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to carnitine, frequently applied as a substrate for fatty acid oxidation (FAO) in mitochondrial preparations.

Abbreviation: Oct

Reference: pdf

MitoPedia topics: Substrate and metabolite

» Fatty acid oxidation

» Carnitine O-octanoyltransferase

Application in HRR

Oct: Octanoylcarnitine (DL-Octanoyl-carnitine-HCL, C₁₅H₃₀NO₄Cl); TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at R.T, desiccate); FW = 323.85.

Alternative: (±)-Octanoylcarnitine chloride, BioTrend, Cat. No. BS0136, 50 mg, store desiccate at RT; FW = 323.86.

Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.

Preparation of 100 mM stock solution (dissolved in H₂O):

Weigh 32.4 mg of octanoylcarnitine and dissolve in 1 ml H₂O.
Divide into 0.2 ml portions.
Store at -20 °C.

Oxygraph-2k manual titrations into 2 ml O2k-chamber

Titration volume: 4 µl using a 10 µl syringe.
Final concentration: 0.2 mM in MiR05 or MiR06. Optimum Oct concentrations have to be evaluated, particularly in media with BSA concentrations different from MiR06.

@@ Line 1: / Line 1: @@
 {{MitoPedia
 |abbr=Oct
-|description='''Octanoylcarnitine''' is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to [[carnitine]], frequently applied as a substrate for [[fatty acid oxidation]] (FAO) of [[mitochondrial preparations]].
+|description='''Octanoylcarnitine''' is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to [[carnitine]], frequently applied as a substrate for [[fatty acid oxidation]] (FAO) in [[mitochondrial preparations]].
 |info=[http://www.jbc.org/content/256/16/8371.full.pdf  ''pdf'']
 |type=Substrate ETS
@@ Line 11: / Line 11: @@
 |type=Substrate ETS
 }}
-'''Carnitine O-octanoyltransferase''' is an enzyme that catalyzes the chemical reaction:
+:» [[Fatty acid oxidation]]
-::''octanoyl-CoA + L-carnitine ↔ CoA + L-octanoylcarnitine''
+:» [[Carnitine O-octanoyltransferase]]
-Thus, the two substrates of this enzyme are octanoyl-CoA and L-carnitine, whereas its two products are CoA and L-octanoylcarnitine.
 == Application in [[HRR]] ==
@@ Line 37: / Line 33: @@
 ::* Titration volume: 4 µl using a 10 µl syringe.
-::* Final concentration: 0.2 mM in MiR05 or [[MiR06]]. Optimum Oct concentration has to be evaluated, particularly in media with BSA concentrations different from MiR06.
+::* Final concentration: 0.2 mM in MiR05 or [[MiR06]]. Optimum Oct concentrations have to be evaluated, particularly in media with BSA concentrations different from MiR06.