Difference between revisions of "Octanoylcarnitine"

Revision as of 11:55, 28 March 2024

high-resolution terminology - matching measurements at high-resolution

Octanoylcarnitine

Description

Octanoylcarnitine is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to carnitine, frequently applied as a substrate for fatty acid oxidation (FAO) in mitochondrial preparations.

Abbreviation: Oct

Reference: pdf

MitoPedia topics: Substrate and metabolite

» Fatty acid oxidation

» Carnitine O-octanoyltransferase

Application in HRR

Oct: Octanoylcarnitine ((±)-Octanoylcarnitine chloride; C₁₅H₃₀NO₄Cl), BioTrend (brand: APExBIO Technology): B6371-50 mg, store at -20 °C, CAS: 18822-86-1, M = 323.86 g·mol^-1

Alternative: TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at RT, desiccate); M = 323.85 g·mol^-1.

General tips: For obtaining a higher solubility, warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.

Preparation of 100 mM stock solution (dissolved in H₂O or DMSO, can differ from batch to batch - check Certificate of Analysis):

Weigh 50 mg of octanoylcarnitine and dissolve in 1543 µL H₂O or DMSO.
Divide into 0.2 mL portions.
Store at -20 °C.

Note 2024-03-26: According to the information provided by the supplier (BioTrend) is the solubility of Octanoylcarnitine in DMSO and H₂O comparable ( ≥36.9 mg/mL in DMSO; ≥18.6 mg/mL in EtOH; ≥37.4 mg/mL in H₂O), therefore we consider more rational approach to use H₂O as a solvent in OROBOROS Lab because of better handling at room temperature. Disadvantage of DMSO: freezing point of +18.55 °C (65.4 °F). When using DMSO as solvent, make sure to bring the stock solution to room temperature before filling the syringe, which is important to avoid blocking the needle.

communicated by Sobotka Ondrej (talk) 17:46, 26 March 2024 (CET)

» O2k manual titrations into 2 mL O2k-chamber

Titration volume (2-mL O2k-chamber): 10 µL using a 25 µL Hamilton syringe.
Titration volume (0.5-mL O2k-chamber): 2.5 µL using a 10 µL Hamilton syringe
Final concentration: 0.5 mM in MiR05 or MiR06. Optimum Oct concentrations have to be evaluated, particularly in media with BSA concentrations different from MiR05.

Labels:

MitoPedia:FAT4BRAIN

@@ Line 1: / Line 1: @@
 {{MitoPedia
 |abbr=Oct
-|description='''Octanoyl carnitine''' is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to [[carnitine]].
+|description='''Octanoylcarnitine''' is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to [[carnitine]], frequently applied as a substrate for [[fatty acid oxidation]] (FAO) in [[mitochondrial preparations]].
 |info=[http://www.jbc.org/content/256/16/8371.full.pdf  ''pdf'']
-|type=Substrate ETS
 }}
+{{MitoPedia concepts}}
+{{MitoPedia methods}}
+{{MitoPedia O2k and high-resolution respirometry}}
 {{MitoPedia topics
 |mitopedia topic=Substrate and metabolite
-|type=Substrate ETS
 }}
+:» [[Fatty acid oxidation]]
+:» [[Carnitine O-octanoyltransferase]]
+__TOC__
+== Application in [[HRR]] ==
+{{Chemical_description
+|abbr=Oct
+|trivial name=Octanoylcarnitine
+|complete name=(±)-Octanoylcarnitine chloride
+|chem formula=C<sub>15</sub>H<sub>30</sub>NO<sub>4</sub>Cl
+|molar mass=323.86
+|vendor=BioTrend (brand: APExBIO Technology)
+|product number=B6371-50 mg
+|store at=-20 °C
+|sensitivity=
+|cas=18822-86-1
+|h statements=
+|h info=
+}}<!--:::: '''Oct: Octanoylcarnitine''' ((±)-Octanoylcarnitine chloride, C<sub>15</sub>H<sub>30</sub>NO<sub>4</sub>Cl); BioTrend (brand: APExBIO Technology) Cat. No. B6371, 50 mg, store at -20°C; M = 323.86 g·mol<sup>-1</sup>.-->
+:::: '''Alternative:''' <!--(±)-Octanoylcarnitine chloride, BioTrend, Cat. No. BS0136, 50 mg, store desiccate at RT; M = 323.86 g·mol<sup>-1</sup>.--> TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at RT, desiccate); M = 323.85 g·mol<sup>-1</sup>.
+:::: '''General tips:''' For obtaining a higher solubility, warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
-'''Carnitine O-octanoyltransferase''' is an enzyme that catalyzes the chemical reaction:
-octanoyl-CoA + L-carnitine ↔  CoA + L-octanoylcarnitine
-Thus, the two substrates of this enzyme are octanoyl-CoA and L-carnitine, whereas its two products are CoA and L-octanoylcarnitine.
+:::: '''Preparation of 100 mM stock solution''' (dissolved in H<sub>2</sub>O or DMSO, can differ from batch to batch - check Certificate of Analysis):
+::::# Weigh 50 mg of octanoylcarnitine and dissolve in 1543 µL H<sub>2</sub>O or DMSO.
+::::# Divide into 0.2 mL portions.
+::::# Store at -20 °C.
-== Application in [[HRR]] ==
+:::::* '''''Note 2024-03-26:''''' According to the information provided by the supplier (BioTrend) is the solubility of Octanoylcarnitine in DMSO and H<sub>2</sub>O comparable ( ≥36.9 mg/mL in DMSO; ≥18.6 mg/mL in EtOH; ≥37.4 mg/mL in H<sub>2</sub>O), therefore we consider more rational approach to use H<sub>2</sub>O as a solvent in OROBOROS Lab because of better handling at room temperature. Disadvantage of DMSO: freezing point of +18.55 °C (65.4 °F). When using DMSO as solvent, make sure to bring the stock solution to room temperature before filling the syringe, which is important to avoid blocking the needle.
-'''Oct: Octanoyl carnitine''' (DL-Octanoyl-carnitine-HCL, C<sub>15</sub>H<sub>30</sub>NO<sub>4</sub>Cl); TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at R.T, desiccate); FW = 323.85.
+:::::: communicated by [[User:Sobotka Ondrej|Sobotka Ondrej]] ([[User talk:Sobotka Ondrej|talk]]) 17:46, 26 March 2024 (CET)
+:::» '''O2k manual titrations into 2 mL O2k-chamber'''
-<span  style="color:#8B008B">  '''Caution:''' Chemicals stored in the fridge  or freezer should be  allowed to reach room temperature before  opening.</span>
+::::* Titration volume ('''2-mL O2k-chamber'''): 10 µL using a 25 µL Hamilton syringe.
+::::* Titration volume ('''0.5-mL O2k-chamber'''): 2.5 µL using a 10 µL Hamilton syringe
+::::* Final concentration: 0.5 mM in [[MiR05-Kit|MiR05]] or [[MiR06]]. Optimum Oct concentrations have to be evaluated, particularly in media with BSA concentrations different from MiR05.
-'''Preparation of 100 mM stock solution''' (dissolved in H<sub>2</sub>O):
-::1) weigh 32.4 mg of octanoyl carnitine and dissolve in 1 ml H<sub>2</sub>O
+{{Labeling
-::2) divide into 0.2 ml portions
+|additional=MitoPedia:FAT4BRAIN
-::3) store frozen at -20 °C.
+}}