Difference between revisions of "Octanoylcarnitine"

Revision as of 23:01, 4 January 2018

high-resolution terminology - matching measurements at high-resolution

Octanoylcarnitine

Description

Octanoylcarnitine is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to carnitine, frequently applied as a substrate for fatty acid oxidation (FAO) in mitochondrial preparations.

Abbreviation: Oct

Reference: pdf

MitoPedia topics: Substrate and metabolite

» Fatty acid oxidation

» Carnitine O-octanoyltransferase

Application in HRFR

Oct: Octanoylcarnitine (DL-Octanoyl-carnitine-HCL, C₁₅H₃₀NO₄Cl); TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at RT, desiccate); FW = 323.85.

Alternative: (±)-Octanoylcarnitine chloride, BioTrend, Cat. No. BS0136, 50 mg, store desiccate at RT; FW = 323.86.

Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.

Preparation of 100 mM stock solution (dissolved in H₂O):

Weigh 50 mg of octanoylcarnitine and dissolve in 1543 µL H₂O.
Divide into 0.2 mL portions.
Store at -20 °C.

O2k manual titrations into 2 mL O2k-chamber

Titration volume: 4 µL using a 10 µL syringe.
Final concentration: 0.2 mM in MiR05 or MiR06. Optimum Oct concentrations have to be evaluated, particularly in media with BSA concentrations different from MiR05.

@@ Line 3: / Line 3: @@
 |description='''Octanoylcarnitine''' is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to [[carnitine]], frequently applied as a substrate for [[fatty acid oxidation]] (FAO) in [[mitochondrial preparations]].
 |info=[http://www.jbc.org/content/256/16/8371.full.pdf  ''pdf'']
-|type=Substrate ETS
-}}
-{{MitoPedia methods|type=Substrate ETS
 }}
 {{MitoPedia topics
 |mitopedia topic=Substrate and metabolite
-|type=Substrate ETS
 }}
 :» [[Fatty acid oxidation]]
@@ Line 16: / Line 12: @@
 == Application in [[HRFR]] ==
-'''Oct: Octanoylcarnitine''' (DL-Octanoyl-carnitine-HCL, C<sub>15</sub>H<sub>30</sub>NO<sub>4</sub>Cl); TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at R.T, desiccate); FW = 323.85.
+:::: '''Oct: Octanoylcarnitine''' (DL-Octanoyl-carnitine-HCL, C<sub>15</sub>H<sub>30</sub>NO<sub>4</sub>Cl); TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at RT, desiccate); FW = 323.85.
-'''Alternative:''' (±)-Octanoylcarnitine chloride, BioTrend, Cat. No. BS0136, 50 mg, store desiccate at RT; FW = 323.86.
+:::: '''Alternative:''' (±)-Octanoylcarnitine chloride, BioTrend, Cat. No. BS0136, 50 mg, store desiccate at RT; FW = 323.86.
@@ Line 24: / Line 20: @@
-'''Preparation of 100 mM stock solution''' (dissolved in H<sub>2</sub>O):
+::: '''Preparation of 100 mM stock solution''' (dissolved in H<sub>2</sub>O):
-::# Weigh 50 mg of octanoylcarnitine and dissolve in 1543 µl H<sub>2</sub>O.
+::::# Weigh 50 mg of octanoylcarnitine and dissolve in 1543 µL H<sub>2</sub>O.
-::# Divide into 0.2 ml portions.
+::::# Divide into 0.2 mL portions.
-::# Store at -20 °C.
+::::# Store at -20 °C.
-'''O2k manual titrations into 2 ml O2k-chamber'''
+::: '''O2k manual titrations into 2 mL O2k-chamber'''
-::* Titration volume: 4 µl using a 10 µl syringe.
+::::* Titration volume: 4 µL using a 10 µL syringe.
-::* Final concentration: 0.2 mM in MiR05 or [[MiR06]]. Optimum Oct concentrations have to be evaluated, particularly in media with BSA concentrations different from MiR06.
+::::* Final concentration: 0.2 mM in [[MiR05-Kit|MiR05]] or [[MiR06]]. Optimum Oct concentrations have to be evaluated, particularly in media with BSA concentrations different from MiR05.