Difference between revisions of "Coupling-control state"
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|info=[[Gnaiger 2009 Int J Biochem Cell Biol]] | |info=[[Gnaiger 2009 Int J Biochem Cell Biol]] | ||
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[[File:EPL-free and excess.jpg|right|300px|thumb|[[Gnaiger 2014 MitoPathways |The Blue Book 2014: Fig. 2.4.]]]] | [[File:EPL-free and excess.jpg|right|300px|thumb|[[Gnaiger 2014 MitoPathways |The Blue Book 2014: Fig. 2.4.]]]] | ||
'''Coupling control states:''' [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link=ET-capacity|ET-capacity]] [[Image:L.jpg|link=LEAK respiration|LEAK]] - [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]] | '''Coupling control states:''' [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link=ET-capacity|ET-capacity]] [[Image:L.jpg|link=LEAK respiration|LEAK]] - [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]] | ||
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ย Communicated by [[Gnaiger E]] 2011-02-25, edited 2019-03-08. | ย Communicated by [[Gnaiger E]] 2011-02-25, edited 2019-03-08. | ||
== [[SUITbrowser]] question: Coupling control == | == [[SUITbrowser]] question: Coupling control == | ||
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:::: Use the [https://suitbrowser.oroboros.at/ SUITbrowser] to find the best protocol to answer this and other research questions. | :::: Use the [https://suitbrowser.oroboros.at/ SUITbrowser] to find the best protocol to answer this and other research questions. | ||
{{Keywords: Uncoupling}} | |||
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{{MitoPedia concepts | {{MitoPedia concepts |
Revision as of 07:44, 19 February 2020
Description
Coupling control states are defined in mitochondrial preparations (isolated mitochondria, permeabilized cells, permeabilized tissues, homogenates) as LEAK, OXPHOS, and ET-pathway states, with corresponding respiration rates (L, P, E) in any electron transfer-pathway state which is competent for electron transfer. These coupling states are induced by application of specific inhibitors of the phosphorylation system, titration of ADP and uncouplers. In living cells, the coupling control states are LEAK, ROUTINE, and ET-pathway states of respiration (L, R, E), using membrane-permeable inhibitors of the phosphorylation system (e.g. oligomycin) and uncouplers (e.g. CCCP). Coupling control protocols induce these coupling control states sequentially at a constant electron transfer-pathway state.
Abbreviation: CCS
Reference: Gnaiger 2009 Int J Biochem Cell Biol
Communicated by Gnaiger E 2011-02-25, edited 2019-03-08.
SUITbrowser question: Coupling control
- Coupling control protocols, besides other SUIT protocols, allow to analyze coupling control in living cells or mitochondrial preparations.
- Use the SUITbrowser to find the best protocol to answer this and other research questions.
- Bioblast links: Uncoupling - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Specific
- ยป Artefacts by single dose uncoupling
- ยป ATP synthase
- ยป CCCP
- ยป Coupling-control protocol
- ยป DNP
- ยป Dyscoupled respiration
- ยป FCCP
- ยป Is respiration uncoupled - noncoupled - dyscoupled?
- ยป Noncoupled respiration: Discussion
- ยป Uncoupler
- ยป Uncoupled respiration - see ยป Noncoupled respiration
- ยป Uncoupling proteins
- ยป Uncoupling protein 1
- ยป Uncoupler titrations - Optimum uncoupler concentration
- Specific
- Respiratory states and control ratios
- ยป Biochemical coupling efficiency
- ยป Coupling-control state
- ยป Electron-transfer-pathway state
- ยป Electron-transfer pathway
- ET capacity
- ยป E-L coupling efficiency
- ยป Flux control efficiency
- ยป Flux control ratio
- ยป LEAK-control ratio
- ยป LEAK respiration
- ยป Noncoupled respiration
- ยป OXPHOS
- ยป OXPHOS capacity; ยป State 3
- ยป OXPHOS-control ratio, P/E ratio
- ยป Respiratory acceptor control ratio
- ยป ROUTINE-control ratio
- ยป ROUTINE respiration
- ยป ROUTINE state
- ยป State 3u
- ยป State 4
- ยป Uncoupling-control ratio UCR
- Respiratory states and control ratios
- Gnaiger E et al โ MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1
- Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002
- General (alphabetical order)
- Other keyword lists
MitoPedia concepts:
MiP concept,
Respiratory state,
SUIT concept,
SUIT state,
Recommended
MitoPedia methods:
Respirometry
MitoPedia topics:
EAGLE