Description
2,4-dinitrophenol (C6H4N2O5; FW = 184.11) is a protonophore acting as an uncoupler of oxidative phosphorylation.
Abbreviation: DNP
Reference: Steinlechner-Maran 1996 Am J Physiol Cell Physiol
MitoPedia topics: Uncoupler
Preparation of 10 mM DNP stock solution (dissolved in H2O):
Caution: Toxic!
- 1. Weigh 3.7 mg of DNP (FW = 184.11).
- 2. Dissolve in 1.2 ml H2O.
- 3. Neutralize with 1 N KOH.
- 4. Adjust final volume to 2 ml and divide into 0.2 ml portions.
- 6. Store at -20 Β°C.