Wang 2016 Toxicol Sci: Difference between revisions
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{{Labeling | {{Labeling | ||
|area=Respiration, Comparative MiP;environmental MiP, Pharmacology;toxicology | |area=Respiration, Comparative MiP;environmental MiP, Pharmacology;toxicology | ||
| | |organism=Fishes | ||
|tissues=Endothelial;epithelial;mesothelial cell | |tissues=Endothelial;epithelial;mesothelial cell | ||
|preparations=Permeabilized tissue | |preparations=Permeabilized tissue | ||
|couplingstates=LEAK, OXPHOS, | |couplingstates=LEAK, OXPHOS, ET | ||
| | |pathways=N, S, CIV, NS, ROX | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
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Latest revision as of 16:30, 13 November 2017
Wang L, Espinoza HM, MacDonald JW, Bammler TK, Williams CR, Yeh A, Louie KW, Marcinek DJ, Gallagher EP (2016) Olfactory Transcriptional Analysis of Salmon Exposed to Mixtures of Chlorpyrifos and Malathion Reveal Novel Molecular Pathways of Neurobehavioral Injury. Toxicol Sci 149:145-57. |
Wang L, Espinoza HM, MacDonald JW, Bammler TK, Williams CR, Yeh A, Louie KW, Marcinek DJ, Gallagher EP (2016) Toxicol Sci
Abstract: Pacific salmon exposed to sublethal concentrations of organophosphate pesticides (OP) have impaired olfactory function that can lead to loss of behaviors that are essential for survival. These exposures often involve mixtures and can occur at levels below those which inhibit acetylcholinesterase (AChE). In this study, juvenile Coho salmon were exposed for 24โh to either 0.1, 0.5, or 2.5โppb chlorpyrifos (CPF), 2, 10, or 50โppb malathion (MAL), or binary mixtures of 0.1 CPF:2โppb MAL, 0.5 CPF:10โppb MAL, or 2.5 CPF:10โppb MAL to mimic single and binary environmental exposures. Microarray analysis of olfactory rosettes from pesticide-exposed salmon revealed differentially expressed genes involved in nervous system function and signaling, aryl hydrocarbon receptor signaling, xenobiotic metabolism, and mitochondrial dysfunction. Coho exposed to OP mixtures exhibited a more pronounced loss in detection of a predatory olfactory cue relative to those exposed to single compounds, whereas respirometry experiments demonstrated that exposure to OPs, individually and in mixtures, reduced maximum respiratory capacity of olfactory rosette mitochondria. The observed molecular, biochemical, and behavioral effects occurred largely in the absence of effects on brain AChE. In summary, our results provide new insights associated with the sublethal neurotoxic effects of OP mixtures relevant to environmental exposures involving molecular and cellular pathways of injury to the salmon olfactory system that underlie neurobehavioral injury. โข Keywords: Chlorpyrifos, Malathion, Salmon, Olfaction, Microarray analysis, Mitochondria
โข O2k-Network Lab: US WA Seattle Marcinek DJ
Labels: MiParea: Respiration, Comparative MiP;environmental MiP, Pharmacology;toxicology
Organism: Fishes
Tissue;cell: Endothelial;epithelial;mesothelial cell
Preparation: Permeabilized tissue
Coupling state: LEAK, OXPHOS, ET
Pathway: N, S, CIV, NS, ROX
HRR: Oxygraph-2k